Abstract:
:A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of toluene carbon was converted to biomass and nonvolatile metabolic by-products, primarily the former. The observed stoichiometric ratio of moles of sulfate consumed per mole of toluene consumed was consistent with the theoretical ratio for mineralization of toluene coupled with the reduction of sulfate to hydrogen sulfide. Strain PRTOL1 also transforms o- and p-xylene to metabolic products when grown with toluene. However, xylene transformation by PRTOL1 is slow relative to toluene degradation and cannot be sustained over time. Stable isotope-labeled substrates were used in conjunction with gas chromatography-mass spectrometry to investigate the by-products of toluene and xylene metabolism. The predominant by-products from toluene, o-xylene, and p-xylene were benzylsuccinic acid, (2-methylbenzyl)succinic acid, and 4-methylbenzoic acid (or p-toluic acid), respectively. Metabolic by-products accounted for nearly all of the o-xylene consumed. Enzyme assays indicated that acetyl coenzyme A oxidation proceeded via the carbon monoxide dehydrogenase pathway. Compared with the only other reported toluene-degrading, sulfate-reducing bacterium, strain PRTOL1 is distinct in that it has a novel 16S rRNA gene sequence and was derived from a freshwater rather than marine environment.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Beller HR,Spormann AM,Sharma PK,Cole JR,Reinhard Mdoi
10.1128/AEM.62.4.1188-1196.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
1188-96issue
4eissn
0099-2240issn
1098-5336journal_volume
62pub_type
杂志文章abstract::Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic ac...
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pub_type: 杂志文章
doi:10.1128/AEM.44.3.597-603.1982
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更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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更新日期:2006-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
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更新日期:1982-03-01 00:00:00