Enzymatic degradation of chlorodiamino-s-triazine.

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:The harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as Australia, Germany, and South Africa, among others. However, conflicting reports on the microbial quality of harvested rainwater have been published. To monitor the presence of potential pathogenic bacteria durin...

journal_title：Applied and environmental microbiology

authors： Dobrowsky PH,De Kwaadsteniet M,Cloete TE,Khan W

更新日期：2014-04-01 00:00:00

abstract：:Mucoromycota representatives are known to harbour two types of endohyphal bacteria (EHB) - Burkholderia related endobacteria (BRE) and Mycoplasma related endobacteria (MRE). While both BRE and MRE occur in fungi representing all subphyla of Mucoromycota, their distribution is not well studied. Therefore, it is difficu...

journal_title：Applied and environmental microbiology

authors： Okrasińska A,Bokus A,Duk K,Gęsiorska A,Sokołowska B,Miłobędzka A,Wrzosek M,Pawłowska J

更新日期：2021-01-22 00:00:00

abstract：:A microscopically pure enrichment culture of a gram-negative anaerobic bacterium, in the present article referred to as PER-K23, was isolated from an anaerobic packed-bed column in which tetrachloroethene (PCE) was reductively transformed to ethane via trichloroethene (TCE), cis-1,2-dichloroethene (cis-1,2-DCE), chlor...

journal_title：Applied and environmental microbiology

authors： Holliger C,Schraa G,Stams AJ,Zehnder AJ

更新日期：1993-09-01 00:00:00

abstract：:Aspergillus oryzae has been used in the food and beverage industry for centuries, and industrial strains have been produced by multiple rounds of selection. Targeted gene deletion technology is particularly useful for strain improvement in such strains, particularly when they do not have a well-characterized meiotic c...

journal_title：Applied and environmental microbiology

authors： Hunter AJ,Morris TA,Jin B,Saint CP,Kelly JM

更新日期：2013-09-01 00:00:00

abstract：:Bacterioplankton are fundamental components of marine ecosystems and influence the entire biosphere by contributing to the global biogeochemical cycles of key elements. Yet, there is a significant gap in knowledge about their diversity and specific activities, as well as environmental factors that shape their communit...

journal_title：Applied and environmental microbiology

authors： Richa K,Balestra C,Piredda R,Benes V,Borra M,Passarelli A,Margiotta F,Saggiomo M,Biffali E,Sanges R,Scanlan DJ,Casotti R

更新日期：2017-08-17 00:00:00

abstract：:We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and even...

journal_title：Applied and environmental microbiology

authors： Sakai S,Imachi H,Sekiguchi Y,Tseng IC,Ohashi A,Harada H,Kamagata Y

更新日期：2009-07-01 00:00:00

abstract：:The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for ...

journal_title：Applied and environmental microbiology

authors： Dupray E,Cormier M

更新日期：1983-11-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...

journal_title：Applied and environmental microbiology

authors： Li GQ,Li SS,Zhang ML,Wang J,Zhu L,Liang FL,Liu RL,Ma T

更新日期：2008-02-01 00:00:00

abstract：:A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

journal_title：Applied and environmental microbiology

authors： Lee C,Langlois BE,Dawson KA

更新日期：1993-05-01 00:00:00

abstract：:Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isot...

journal_title：Applied and environmental microbiology

authors： Sun W,Sun X,Cupples AM

更新日期：2012-04-01 00:00:00

abstract：:A rapid coliphage analysis technique for enumerating coliphages in natural waters has been evaluated by water quality laboratories located throughout the United States. Correlations were established between coliphages and coliforms in natural water systems. These correlations were highly significant. This relationship...

journal_title：Applied and environmental microbiology

authors： Wentsel RS,O'Neill PE,Kitchens JF

更新日期：1982-02-01 00:00:00

abstract：:We previously reported the isolation of a 21.5-kb genomic DNA fragment from Pseudomonas sp. strain ADP, which contains the atzA gene, encoding the first metabolic step for the degradation of the herbicide atrazine (M. de Souza, L. P. Wackett, K. L. Boundy-Mills, R. T. Mandelbaum, and M. J. Sadowsky, Appl. Environ. Mic...

journal_title：Applied and environmental microbiology

authors： Boundy-Mills KL,de Souza ML,Mandelbaum RT,Wackett LP,Sadowsky MJ

更新日期：1997-03-01 00:00:00

abstract：:Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

journal_title：Applied and environmental microbiology

authors： Wang CR,Chu FS

更新日期：1991-04-01 00:00:00

abstract：:The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

journal_title：Applied and environmental microbiology

authors： Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

更新日期：1993-05-01 00:00:00

abstract：:The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

journal_title：Applied and environmental microbiology

authors： Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

更新日期：2019-01-09 00:00:00

abstract：:This study was performed to determine the effects of feeding a fiber-rich fraction of Brassica vegetables on the immune response through changes in enteric bacteria and short-chain fatty acid (SCFA) production in normal mice. The boiled-water-insoluble fraction of Brassica rapa L. (nozawana), which consists mainly of ...

journal_title：Applied and environmental microbiology

authors： Tanaka S,Yamamoto K,Yamada K,Furuya K,Uyeno Y

更新日期：2016-04-18 00:00:00

abstract：:Rhizoremediation of organic chemicals requires high-level expression of biodegradation genes in bacterial strains that are excellent rhizosphere colonizers. Pseudomonas fluorescens F113 is a biocontrol strain that was shown to be an excellent colonizer of numerous plant rhizospheres, including alfalfa. Although a deri...

journal_title：Applied and environmental microbiology

authors： Villacieros M,Whelan C,Mackova M,Molgaard J,Sánchez-Contreras M,Lloret J,Aguirre de Cárcer D,Oruezábal RI,Bolaños L,Macek T,Karlson U,Dowling DN,Martín M,Rivilla R

更新日期：2005-05-01 00:00:00

abstract：:In this study, we compared different methods-including transmission electron microscopy-and various nucleic acid labeling methods in which we used the fluorochromes 4',6'-diamidino-2-phenylindole (DAPI), 4-[3-methyl-2,3-dihydro-(benzo-1, 3-oxazole)-2-methylmethyledene]-1-(3'-trimethyl ammoniumpropyl)-quinilinium diioi...

journal_title：Applied and environmental microbiology

authors： Bettarel Y,Sime-Ngando T,Amblard C,Laveran H

更新日期：2000-06-01 00:00:00

abstract：:The development of high-throughput methods, such as the construction of 18S rRNA gene clone or pyrosequencing libraries, has allowed evaluation of ciliate community composition in hundreds of samples from the rumen and other intestinal habitats. However, several genera of mammalian intestinal ciliates have been descri...

journal_title：Applied and environmental microbiology

authors： Kittelmann S,Devente SR,Kirk MR,Seedorf H,Dehority BA,Janssen PH

更新日期：2015-04-01 00:00:00

abstract：:Duplicate anaerobic fermentor systems were used to examine changes in a community of human fecal bacteria supplied with different carbohydrate energy sources. A panel of group-specific fluorescent in situ hybridization probes targeting 16S rRNA sequences revealed that the fermentors supported growth of a greater propo...

journal_title：Applied and environmental microbiology

authors： Duncan SH,Scott KP,Ramsay AG,Harmsen HJ,Welling GW,Stewart CS,Flint HJ

更新日期：2003-02-01 00:00:00

abstract：:The polyene macrolide antibiotic nystatin produced by Streptomyces noursei contains a deoxyaminosugar mycosamine moiety attached to the C-19 carbon of the macrolactone ring through the beta-glycosidic bond. The nystatin biosynthetic gene cluster contains three genes, nysDI, nysDII, and nysDIII, encoding enzymes with p...

journal_title：Applied and environmental microbiology

authors： Nedal A,Sletta H,Brautaset T,Borgos SE,Sekurova ON,Ellingsen TE,Zotchev SB

更新日期：2007-11-01 00:00:00

abstract：:An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating the mechanisms that underlie their toxicity is critical for developing strategies to overcome them. Here, using...

journal_title：Applied and environmental microbiology

authors： Pisithkul T,Jacobson TB,O'Brien TJ,Stevenson DM,Amador-Noguez D

更新日期：2015-09-01 00:00:00

abstract：:The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded i...

journal_title：Applied and environmental microbiology

authors： Kane SR,Beller HR,Legler TC,Koester CJ,Pinkart HC,Halden RU,Happel AM

更新日期：2001-12-01 00:00:00

abstract：:Gaeumannomyces graminis, the causative agent of take-all disease of wheat, barley, and oats, was detected in infected wheat seedlings by using the polymerase chain reaction to amplify Gaeumannomyces-specific DNA fragments. Nested primers and two rounds of amplification were used to amplify two fragments, approximately...

journal_title：Applied and environmental microbiology

authors： Schesser K,Luder A,Henson JM

更新日期：1991-02-01 00:00:00

abstract：:The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

journal_title：Applied and environmental microbiology

authors： Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

更新日期：1996-06-01 00:00:00

abstract：:Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...

journal_title：Applied and environmental microbiology

authors： Patel UD,Govindarajan P,Dave PJ

更新日期：1989-02-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00