Use of Polymerase Chain Reaction To Detect the Take-All Fungus, Gaeumannomyces graminis, in Infected Wheat Plants.

abstract：:Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...

journal_title：Applied and environmental microbiology

authors： Patel UD,Govindarajan P,Dave PJ

更新日期：1989-02-01 00:00:00

abstract：:Mixed infections have important consequences for the ecology and evolution of host-parasite interactions. In vector-borne diseases, interactions between pathogens occur in both the vertebrate host and the arthropod vector. Spirochete bacteria belonging to the Borrelia burgdorferi sensu lato genospecies complex are tra...

journal_title：Applied and environmental microbiology

authors： Herrmann C,Gern L,Voordouw MJ

更新日期：2013-12-01 00:00:00

abstract：:Demethylation inhibitor (DMI)-resistant strains of the plant pathogenic fungus Penicillium digitatum were shown to be simultaneously resistant to cycloheximide, 4-nitroquinoline-N-oxide (4NQO), and acriflavine. A PMR1 (Penicillium multidrug resistance) gene encoding an ATP-binding cassette (ABC) transporter (P-glycopr...

journal_title：Applied and environmental microbiology

authors： Nakaune R,Adachi K,Nawata O,Tomiyama M,Akutsu K,Hibi T

更新日期：1998-10-01 00:00:00

abstract：:A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

journal_title：Applied and environmental microbiology

authors： Choi Y,Shin H,Lee JH,Ryu S

更新日期：2013-08-01 00:00:00

abstract：:Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

journal_title：Applied and environmental microbiology

authors： Gelsomino R,Vancanneyt M,Cogan TM,Swings J

更新日期：2003-01-01 00:00:00

abstract：:Bacteria in the phylum Acidobacteria are widely distributed and abundant in soils, but their ecological roles are poorly understood, owing in part to a paucity of cultured representatives. In a molecular survey of acidobacterial diversity at the Michigan State University Kellogg Biological Station Long-Term Ecological...

journal_title：Applied and environmental microbiology

authors： Eichorst SA,Breznak JA,Schmidt TM

更新日期：2007-04-01 00:00:00

abstract：:There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...

journal_title：Applied and environmental microbiology

authors： Miller EA,Johnson TJ,Omondi G,Atwill ER,Isbell LA,McCowan B,VanderWaal K

更新日期：2018-12-13 00:00:00

abstract：:The genetic diversity of Desulfovibrio species in environmental samples was determined by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified [NiFe] hydrogenase gene fragments. Five different PCR primers were designed after comparative analysis of [NiFe] hydrogenase gene sequences from three Desulfovibrio ...

journal_title：Applied and environmental microbiology

authors： Wawer C,Muyzer G

更新日期：1995-06-01 00:00:00

abstract：:Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Gong F,Hedden P,Tudzynski B

更新日期：2008-09-01 00:00:00

abstract：:Noroviruses from mussels collected near sewage effluents were compared with local patient outbreak strains. Sequence analyses of RNA polymerase-capsid-poly(A)-3' (3.1-kilobase) regions confirmed the 99.9% similarity between genotype I.1 strains from mussels and patient strains from recreational-bathing outbreaks, indi...

journal_title：Applied and environmental microbiology

authors： Nenonen NP,Hannoun C,Horal P,Hernroth B,Bergström T

更新日期：2008-04-01 00:00:00

abstract：:Escherichia coli is a widely utilized indicator of the sanitary quality of bivalve molluscan shellfish sold for human consumption. However, it is now well documented that shellfish that meet the E. coli standards for human consumption may contain human enteric viruses that cause gastroenteritis and hepatitis. In this ...

journal_title：Applied and environmental microbiology

authors： Doré WJ,Henshilwood K,Lees DN

更新日期：2000-04-01 00:00:00

abstract：:The nor-1 gene in the filamentous fungus Aspergillus parasiticus encodes a ketoreductase involved in aflatoxin biosynthesis. To study environmental influences on nor-1 expression, we generated plasmid pAPGUSNNB containing a nor-1 promoter-beta-glucuronidase (GUS) (encoded by uidA) reporter fusion with niaD (encodes ni...

journal_title：Applied and environmental microbiology

authors： Chiou CH,Miller M,Wilson DL,Trail F,Linz JE

更新日期：2002-01-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:Strains of Xylella fastidiosa isolated from grape, almond, maple, and oleander were characterized by enterobacterial repetitive intergenic consensus sequence-, repetitive extragenic palindromic element (REP)-, and random amplified polymorphic DNA (RAPD)-PCR; contour-clamped homogeneous electric field (CHEF) gel electr...

journal_title：Applied and environmental microbiology

authors： Hendson M,Purcell AH,Chen D,Smart C,Guilhabert M,Kirkpatrick B

更新日期：2001-02-01 00:00:00

abstract：:A total of 442 Listeria isolates, including 234 Listeria seeligeri, 80 L. monocytogenes, 74 L. welshimeri, 50 L. innocua, and 4 L. marthii isolates, were obtained from 1,805 soil, water, and other environmental samples collected over 2 years from four urban areas and four areas representing natural environments. Liste...

journal_title：Applied and environmental microbiology

authors： Sauders BD,Overdevest J,Fortes E,Windham K,Schukken Y,Lembo A,Wiedmann M

更新日期：2012-06-01 00:00:00

abstract：:The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

journal_title：Applied and environmental microbiology

authors： Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

更新日期：1983-10-01 00:00:00

abstract：:In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S ...

journal_title：Applied and environmental microbiology

authors： Duranti S,Turroni F,Milani C,Foroni E,Bottacini F,Dal Bello F,Ferrarini A,Delledonne M,van Sinderen D,Ventura M

更新日期：2013-01-01 00:00:00

abstract：:Ergosterol, a fungus-specific sterol enriched in cell plasma membranes, is an effective antifungal drug target. However, current knowledge of the ergosterol biosynthesis process in the saprophytic human fungal pathogen Aspergillus fumigatus remains limited. In this study, we found that two endoplasmic reticulum-locali...

journal_title：Applied and environmental microbiology

authors： Long N,Xu X,Zeng Q,Sang H,Lu L

更新日期：2017-02-01 00:00:00

abstract：:The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...

journal_title：Applied and environmental microbiology

authors： Limsawat S,Ohgaki S

更新日期：1997-07-01 00:00:00

abstract：:l-Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Rhodotorula rubra has been used in the commercial manufacture of l-phenylalanine from trans-cinnamic acid. In this study, R. graminis PAL was investigated. Mutant strain GX6000 was isolated after ethyl methanesulfonate mutagenesis of wild-type R. graminis GX5007 by ...

journal_title：Applied and environmental microbiology

authors： Orndorff SA,Costantino N,Stewart D,Durham DR

更新日期：1988-04-01 00:00:00

abstract：:The accurate description of a microbial community is an important first step in understanding the roles of its components in ecosystem function. A method for surveying microbial communities termed serial analysis of rRNA genes (SARD) is described here. Through a series of molecular cloning steps, short DNA sequence ta...

journal_title：Applied and environmental microbiology

authors： Ashby MN,Rine J,Mongodin EF,Nelson KE,Dimster-Denk D

更新日期：2007-07-01 00:00:00

abstract：:Bacillus thuringiensis serovar israelensis (B. thuringiensis subsp. israelensis) produces four insecticidal crystal proteins (ICPs) (Cry4A, Cry4B, Cry11A, and Cyt1A). Toxicity of recombinant B. thuringiensis subsp. israelensis strains expressing only one of the toxins was determined with first instars of Tipula paludo...

journal_title：Applied and environmental microbiology

authors： Oestergaard J,Ehlers RU,Martínez-Ramírez AC,Real MD

更新日期：2007-06-01 00:00:00

abstract：:We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcesce...

journal_title：Applied and environmental microbiology

authors： Hover T,Maya T,Ron S,Sandovsky H,Shadkchan Y,Kijner N,Mitiagin Y,Fichtman B,Harel A,Shanks RM,Bruna RE,García-Véscovi E,Osherov N

更新日期：2016-04-18 00:00:00

abstract：:We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

journal_title：Applied and environmental microbiology

authors： Jürgens K,Pernthaler J,Schalla S,Amann R

更新日期：1999-03-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:Psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in Antarctica have been isolated. Phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma Proteobacteria and the Flavobacteria-Cytophaga group. This is the first report ...

journal_title：Applied and environmental microbiology

authors： Gosink JJ,Staley JT

更新日期：1995-09-01 00:00:00

abstract：:Three species of saltmarsh ascomycetes were grown in the presence of all of the constituents of their natural substrate (leaves of cordgrass) or were presented only with aqueous extracts of the leaves. These two growth-condition treatments had no significant effect on total ergosterol content of the fungal mycelia, co...

journal_title：Applied and environmental microbiology

authors： Newell SY

更新日期：1994-09-01 00:00:00

abstract：:White rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. The biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. T...

journal_title：Applied and environmental microbiology

authors： Milstein O,Gersonde R,Huttermann A,Chen MJ,Meister JJ

更新日期：1992-10-01 00:00:00