Metabolic engineering of a novel propionate-independent pathway for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in recombinant Salmonella enterica serovar typhimurium.

abstract：:The scarcity of fresh water in the Mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Mediterranean Psammocinia sp. sponges, the grea...

journal_title：Applied and environmental microbiology

authors： Gal-Hemed I,Atanasova L,Komon-Zelazowska M,Druzhinina IS,Viterbo A,Yarden O

更新日期：2011-08-01 00:00:00

abstract：:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

journal_title：Applied and environmental microbiology

authors： Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

更新日期：1992-12-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

journal_title：Applied and environmental microbiology

authors： Cao Y,Green PG,Holden PA

更新日期：2008-12-01 00:00:00

abstract：:We examined the bacterial communities of epilithic biofilms in 17 streams which represented a gradient ranging from relatively pristine streams to streams highly impacted by acid mine drainage (AMD). A combination of automated ribosomal intergenic spacer analysis with multivariate analysis and ordination provided a se...

journal_title：Applied and environmental microbiology

authors： Lear G,Niyogi D,Harding J,Dong Y,Lewis G

更新日期：2009-06-01 00:00:00

abstract：:Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...

journal_title：Applied and environmental microbiology

authors： Karunakaran R,East AK,Poole PS

更新日期：2013-07-01 00:00:00

abstract：:Restriction fragment length polymorphism and virulence analyses were used to evaluate the population structure of Xanthomonas oryzae pv. oryzae, the rice bacterial blight pathogen, from several rice-growing countries in Asia. Two DNA sequences from X. oryzae pv. oryzae, IS1112, an insertion sequence, and avrXa10, a me...

journal_title：Applied and environmental microbiology

authors： Adhikari TB,Cruz C,Zhang Q,Nelson RJ,Skinner DZ,Mew TW,Leach JE

更新日期：1995-03-01 00:00:00

abstract：:The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

journal_title：Applied and environmental microbiology

authors： Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

更新日期：1983-10-01 00:00:00

abstract：:Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N(2) as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production, photooxygen evolution, and resp...

journal_title：Applied and environmental microbiology

authors： Kumazawa S,Mitsui A

更新日期：1985-08-01 00:00:00

abstract：:The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presen...

journal_title：Applied and environmental microbiology

authors： Valle J,Vadillo S,Piriz S,Gomez-Lucia E

更新日期：1991-03-01 00:00:00

abstract：:Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a "copper switch." At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availabil...

journal_title：Applied and environmental microbiology

authors： Farhan Ul Haque M,Kalidass B,Bandow N,Turpin EA,DiSpirito AA,Semrau JD

更新日期：2015-11-01 00:00:00

abstract：:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

journal_title：Applied and environmental microbiology

authors： Clough SJ,Flavier AB,Schell MA,Denny TP

更新日期：1997-03-01 00:00:00

abstract：:Medicinal plants are valuable resources of natural antimicrobial materials. A novel small protein with antimicrobial activities, designated LJAMP1, was purified from the seeds of a medicinal herb, motherwort (Leonurus japonicus Houtt). LJAMP1 is a heat-stable protein with a molecular mass of 7.8 kDa and a determined i...

journal_title：Applied and environmental microbiology

authors： Yang X,Xiao Y,Wang X,Pei Y

更新日期：2007-02-01 00:00:00

abstract：:Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics...

journal_title：Applied and environmental microbiology

authors： Briczinski EP,Loquasto JR,Barrangou R,Dudley EG,Roberts AM,Roberts RF

更新日期：2009-12-01 00:00:00

abstract：:Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

journal_title：Applied and environmental microbiology

authors： Macalady JL,Mack EE,Nelson DC,Scow KM

更新日期：2000-04-01 00:00:00

abstract：:Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

journal_title：Applied and environmental microbiology

authors： Hewson I,Fuhrman JA

更新日期：2004-06-01 00:00:00

abstract：:Undefined mesophilic mixed (DL) starter cultures are used in the production of continental cheeses and contain unknown strain mixtures of Lactococcus lactis and leuconostocs. The choice of starter culture affects the taste, aroma, and quality of the final product. To gain insight into the diversity of Lactococcus lact...

journal_title：Applied and environmental microbiology

authors： Frantzen CA,Kleppen HP,Holo H

更新日期：2018-01-17 00:00:00

abstract：:Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

journal_title：Applied and environmental microbiology

authors： Pizarro FJ,Jewett MC,Nielsen J,Agosin E

更新日期：2008-10-01 00:00:00

abstract：:Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host...

journal_title：Applied and environmental microbiology

authors： Ryu H,Griffith JF,Khan IU,Hill S,Edge TA,Toledo-Hernandez C,Gonzalez-Nieves J,Santo Domingo J

更新日期：2012-03-01 00:00:00

abstract：:Both Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis produce mosquitocidal toxins during sporulation and are extensively used in the field for control of mosquito populations. All the known toxins of the latter organism are known to be encoded on a large plasmid, pBtoxis. In an attempt to combine the...

journal_title：Applied and environmental microbiology

authors： Gammon K,Jones GW,Hope SJ,de Oliveira CM,Regis L,Silva Filha MH,Dancer BN,Berry C

更新日期：2006-03-01 00:00:00

abstract：:It is now possible to obtain accurate total counts of the bacteria of natural waters with the use of acridine orange staining and epifluorescence microscopy. This approach can be coupled to highly sensitive measurements of heterotrophic activity using radioisotopes. To accomplish this, three variations of a "specific ...

journal_title：Applied and environmental microbiology

authors： Wright RT

更新日期：1978-08-01 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:We report on the characterization of a group of seven novel Brucella strains isolated in 1964 from three native rodent species in North Queensland, Australia, during a survey of wild animals. The strains were initially reported to be Brucella suis biovar 3 on the basis of microbiological test results. Our results indi...

journal_title：Applied and environmental microbiology

authors： Tiller RV,Gee JE,Frace MA,Taylor TK,Setubal JC,Hoffmaster AR,De BK

更新日期：2010-09-01 00:00:00

abstract：:In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene fr...

journal_title：Applied and environmental microbiology

authors： Lampinen J,Virta M,Karp M

更新日期：1995-08-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:The importance of viruses in marine microbial ecology has been established over the past decade. Specifically, viruses influence bacterial abundance and community composition through lysis and alter bacterial genetic diversity through transduction and lysogenic conversion. By contrast, the abundance and distribution o...

journal_title：Applied and environmental microbiology

authors： Williamson KE,Radosevich M,Wommack KE

更新日期：2005-06-01 00:00:00

abstract：:The microbial transformation of [N-methyl-(sup14)C]aldicarb, a carbamate pesticide, occurred in aquifer, lake, and salt marsh sediments. Microbial degradation of aldicarb took place within 21 days in aquifer sediments from sites previously exposed to aldicarb (Jamesport, Long Island, N.Y.) but did not occur in sedimen...

journal_title：Applied and environmental microbiology

authors： Kazumi J,Capone DG

更新日期：1995-08-01 00:00:00