Abstract:
:Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics demands techniques with high discriminatory power to differentiate among strains. However, to date, molecular approaches, such as pulsed-field gel electrophoresis and randomly amplified polymorphic DNA-PCR, have been ineffective at achieving strain separation due to the monomorphic nature of this subspecies. Previously, sequencing and comparison of two B. animalis subsp. lactis genomes (DSMZ 10140 and Bl-04) confirmed this high level of sequence similarity, identifying only 47 single-nucleotide polymorphisms (SNPs) and four insertions and/or deletions (INDELs) between them. In this study, we hypothesized that a sequence-based typing method targeting these loci would permit greater discrimination between strains than previously attempted methods. Sequencing 50 of these loci in 24 strains of B. animalis subsp. lactis revealed that a combination of nine SNPs/INDELs could be used to differentiate strains into 14 distinct genotypic groups. In addition, the presence of a nonsynonymous SNP within the gene encoding a putative glucose uptake protein was found to correlate with the ability of certain strains to transport glucose and to grow rapidly in a medium containing glucose as the sole carbon source. The method reported here can be used in clinical, regulatory, and commercial applications requiring identification of B. animalis subsp. lactis at the strain level.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Briczinski EP,Loquasto JR,Barrangou R,Dudley EG,Roberts AM,Roberts RFdoi
10.1128/AEM.01430-09subject
Has Abstractpub_date
2009-12-01 00:00:00pages
7501-8issue
23eissn
0099-2240issn
1098-5336pii
AEM.01430-09journal_volume
75pub_type
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doi:10.1128/AEM.49.1.198-200.1985
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.1.260-264.1983
更新日期:1983-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.4.919-922.1980
更新日期:1980-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.2.349-356.1982
更新日期:1982-02-01 00:00:00