Microbial communities from methane hydrate-bearing deep marine sediments in a forearc basin.

Abstract:

:Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell numbers generally decreased with sediment depth. Lipid biomarker analyses indicated the presence of viable biomass at concentrations greater than previously reported for terrestrial subsurface environments at similar depths. Archaeal lipids were more abundant than bacterial lipids. Methane was produced from both acetate and hydrogen in enrichments inoculated with sediment from all depths evaluated, at both 10 and 35 degrees C. Characterization of 16S rRNA genes amplified from the sediments indicated that archaeal clones could be discretely grouped within the Euryarchaeota and Crenarchaeota domains. The bacterial clones exhibited greater overall diversity than the archaeal clones, with sequences related to the Bacteroidetes, Planctomycetes, Actinobacteria, Proteobacteria, and green nonsulfur groups. The majority of the bacterial clones were either members of a novel lineage or most closely related to uncultured clones. The results of these analyses suggest that the microbial community in this environment is distinct from those in previously characterized methane hydrate-bearing sediments.

journal_name

Appl Environ Microbiol

authors

Reed DW,Fujita Y,Delwiche ME,Blackwelder DB,Sheridan PP,Uchida T,Colwell FS

doi

10.1128/aem.68.8.3759-3770.2002

keywords:

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

3759-70

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

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