Abstract:
:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the light chain of the toxin. Although, nonspecific priming was observed when the PCR protocol was tested with other clostridial species, only the PCR product from C. botulinum type B isolates reacted with the radiolabeled internal probe. As little as 100 fg of DNA (approximately 35 clostridial cells) could be detected after only 25 amplification cycles.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Szabo EA,Pemberton JM,Desmarchelier PMdoi
10.1128/AEM.58.1.418-420.1992keywords:
subject
Has Abstractpub_date
1992-01-01 00:00:00pages
418-20issue
1eissn
0099-2240issn
1098-5336journal_volume
58pub_type
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