Specific detection of Clostridium botulinum type B by using the polymerase chain reaction.

Abstract:

:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the light chain of the toxin. Although, nonspecific priming was observed when the PCR protocol was tested with other clostridial species, only the PCR product from C. botulinum type B isolates reacted with the radiolabeled internal probe. As little as 100 fg of DNA (approximately 35 clostridial cells) could be detected after only 25 amplification cycles.

journal_name

Appl Environ Microbiol

authors

Szabo EA,Pemberton JM,Desmarchelier PM

doi

10.1128/AEM.58.1.418-420.1992

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

418-20

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

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