Abstract:
:Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (CYP51) and of the ribosomal DNA internal transcribed spacer 1 (ITS1), ITS2, and 5. 8S regions. We identified four point mutations (three in CYP51 and one in ITS1) that distinguished groups I and II from group III based on a sample of 132 single-spore isolates originating from Europe, Tunisia, Israel, India, and Australia. We developed a nested allele-specific PCR assay in which the CYP51 point mutations were used to detect and distinguish groups I and II from group III in crude mildewed samples from vineyards. In a preliminary study performed with samples from French vineyards in which isolates belonging to genetic groups I and III were present, we found that a shift from a population composed primarily of group I isolates to a population composed primarily of group III isolates occurred during the grapevine growing season.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Délye C,Ronchi V,Laigret F,Corio-Costet MFdoi
10.1128/AEM.65.9.3950-3954.1999keywords:
subject
Has Abstractpub_date
1999-09-01 00:00:00pages
3950-4issue
9eissn
0099-2240issn
1098-5336journal_volume
65pub_type
杂志文章abstract::The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates. This assumption is based solely on early chemical evidence (B. H. Davies, Biochem. J. 116:93-99, 1...
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doi:10.1128/AEM.00545-12
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abstract::Diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. In this study, a long-term placebo-controlled cro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00233-06
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abstract::Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1380-1387.2006
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.1.221-229.1996
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.2.420-428.1996
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pub_type: 杂志文章
doi:10.1128/AEM.00691-09
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.7.3108-3113.1999
更新日期:1999-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.2.613-618.1994
更新日期:1994-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00448-11
更新日期:2011-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02851-07
更新日期:2008-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3483-3485.1996
更新日期:1996-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.38.1.173-174.1979
更新日期:1979-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.06811-11
更新日期:2012-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1374-1378.1985
更新日期:1985-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.5.1205-1207.1982
更新日期:1982-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.10.3639-3644.1995
更新日期:1995-10-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.8.2031-2036.1988
更新日期:1988-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.6.2683-2689.2002
更新日期:2002-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2268-2270.1990
更新日期:1990-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.11.3346-3353.1990
更新日期:1990-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00314-09
更新日期:2009-06-01 00:00:00
abstract::With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.4.886-887.1984
更新日期:1984-04-01 00:00:00
abstract::Media used to determine the MICs of heavy metals for bacteria are unreliable because organic components in the media bind or chelate most of the metal being studied. To define specific metal activity in media and to maintain metal activity at a constant level, HEPES-MES [N-2-hydroxyethylpiperazine-N' -2-ethanesulfonic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.8.2101-2104.1989
更新日期:1989-08-01 00:00:00
abstract::Male-specific RNA coliphages (F-RNA coliphages) have been proposed as a potential viral indicator of fecal contamination in water and foods because they are easy to culture and are a normal component of the mammalian gut flora. F-RNA coliphage plaque numbers are typically obtained by directly plating a 10-fold dilutio...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00436-09
更新日期:2009-10-01 00:00:00
abstract::A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.2.212-219.1985
更新日期:1985-08-01 00:00:00
abstract::Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.5.1026-1030.1986
更新日期:1986-11-01 00:00:00
abstract::Mono Lake is an alkaline hypersaline lake that supports high methane oxidation rates. Retrieved pmoA sequences showed a broad diversity of aerobic methane oxidizers including the type I methanotrophs Methylobacter (the dominant genus), Methylomicrobium, and Methylothermus, and the type II methanotroph Methylocystis. S...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.10.6458-6462.2005
更新日期:2005-10-01 00:00:00
abstract::The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.6.1908-1912.1996
更新日期:1996-06-01 00:00:00
abstract::The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.8.4736-4743.2005
更新日期:2005-08-01 00:00:00