Significant enhanced expression and solubility of human proteins in Escherichia coli by fusion with protein S from Myxococcus xanthus.

abstract：:Fourteen pesticides (fungicides, herbicides, and insecticides) were tested to determine whether they had deleterious effects on the bioinsecticide Bacillus popilliae, the causal agent of milky disease. All of these pesticides reduced levels of spore viability, spore germination, and/or vegetative cell growth when they...

journal_title：Applied and environmental microbiology

authors： Dingman DW

更新日期：1994-07-01 00:00:00

abstract：:A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of ...

journal_title：Applied and environmental microbiology

authors： Nomura M,Kobayashi M,Okamoto T

更新日期：2002-05-01 00:00:00

abstract：:Cultures of 10 different bacteria were used to serve as food sources for axenically grown Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis. The nonpigmented enterobacteriaceae Escherichia coli K-12 and Klebsiella aerogenes appeared to be excellent feed to all three amoebae. Hardly any gro...

journal_title：Applied and environmental microbiology

authors： Weekers PH,Bodelier PL,Wijen JP,Vogels GD

更新日期：1993-07-01 00:00:00

abstract：:In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

journal_title：Applied and environmental microbiology

authors： Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

更新日期：2012-07-01 00:00:00

abstract：:Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a defined deletion o...

journal_title：Applied and environmental microbiology

authors： Yurgel SN,Mortimer MW,Rice JT,Humann JL,Kahn ML

更新日期：2013-03-01 00:00:00

abstract：:Fusarium fungal contaminants and related mycotoxins were investigated in eight maize feed samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. Fusarium moniliforme, F. proliferatum, and F. subglutinans were isolated from seven, eight, and five samples, respectively. These strains belonged t...

journal_title：Applied and environmental microbiology

authors： Munkvold G,Stahr HM,Logrieco A,Moretti A,Ritieni A

更新日期：1998-10-01 00:00:00

abstract：:Thiobacillus denitrificans is a widespread, chemolithoautotrophic bacterium with an unusual and environmentally relevant metabolic repertoire, which includes its ability to couple denitrification to sulfur compound oxidation; to catalyze anaerobic, nitrate-dependent oxidation of Fe(II) and U(IV); and to oxidize minera...

journal_title：Applied and environmental microbiology

authors： Letain TE,Kane SR,Legler TC,Salazar EP,Agron PG,Beller HR

更新日期：2007-05-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...

journal_title：Applied and environmental microbiology

authors： Johnson CN,Bowers JC,Griffitt KJ,Molina V,Clostio RW,Pei S,Laws E,Paranjpye RN,Strom MS,Chen A,Hasan NA,Huq A,Noriea NF 3rd,Grimes DJ,Colwell RR

更新日期：2012-10-01 00:00:00

abstract：:We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. col...

journal_title：Applied and environmental microbiology

authors： Salinger N,Kokona B,Fairman R,Okeke IN

更新日期：2009-01-01 00:00:00

abstract：:Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

journal_title：Applied and environmental microbiology

authors： Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

更新日期：2020-06-17 00:00:00

abstract：:Contamination of fresh produce with pathogenic Escherichia coli, including Shiga-toxigenic E. coli (STEC), represents a serious risk to human health. Colonization is governed by multiple bacterial and plant factors that can impact the probability and suitability of bacterial growth. Thus, we aimed to determine whether...

journal_title：Applied and environmental microbiology

authors： Merget B,Forbes KJ,Brennan F,McAteer S,Shepherd T,Strachan NJC,Holden NJ

更新日期：2019-05-16 00:00:00

abstract：:During submerged culture in the presence of glucose and glutamate, the filamentous fungus Monascus ruber produces water-soluble red pigments together with citrinin, a mycotoxin with nephrotoxic and hepatoxic effects on animals. Analysis of the (13)C-pigment molecules from mycelia cultivated with [1-(13)C]-, [2-(13)C]-...

journal_title：Applied and environmental microbiology

authors： Hajjaj H,Klaébé A,Goma G,Blanc PJ,Barbier E,François J

更新日期：2000-03-01 00:00:00

abstract：:Alteromonas is a widely distributed genus of marine Gammaproteobacteria, with representatives shown to be key players in diverse processes, including biogeochemical cycling and biofouling of marine substrata. While Alteromonas spp. are early colonizers of copper-based antifouling paints on marine vessels, their mechan...

journal_title：Applied and environmental microbiology

authors： Cusick KD,Polson SW,Duran G,Hill RT

更新日期：2020-01-21 00:00:00

abstract：:While filamentous cyanobacteria play a crucial role in food web dynamics and biogeochemical cycling of many aquatic ecosystems around the globe, the knowledge regarding the phages infecting them is limited. Here, we describe the complete genome of the virulent cyanophage vB_AphaS-CL131 (here, CL 131), a Siphoviridae p...

journal_title：Applied and environmental microbiology

authors： Šulčius S,Šimoliūnas E,Alzbutas G,Gasiūnas G,Jauniškis V,Kuznecova J,Miettinen S,Nilsson E,Meškys R,Roine E,Paškauskas R,Holmfeldt K

更新日期：2018-12-13 00:00:00

abstract：:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

journal_title：Applied and environmental microbiology

authors： Russell EG

更新日期：1979-02-01 00:00:00

abstract：:The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

journal_title：Applied and environmental microbiology

authors： van Niel EW,Gottschal JC

更新日期：1998-03-01 00:00:00

abstract：:Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...

journal_title：Applied and environmental microbiology

authors： Heinänen A,Kuparinen J

更新日期：1991-11-01 00:00:00

abstract：:Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

journal_title：Applied and environmental microbiology

authors： Liu W,Hansen JN

更新日期：1990-08-01 00:00:00

abstract：:The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

journal_title：Applied and environmental microbiology

authors： Wei X,Bauer WD

更新日期：1998-05-01 00:00:00

abstract：:Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...

journal_title：Applied and environmental microbiology

authors： Napolitano JJ

更新日期：1982-07-01 00:00:00

abstract：:A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

更新日期：1998-07-01 00:00:00

abstract：:AbiV is an abortive infection protein that inhibits the lytic cycle of several virulent phages infecting Lactococcus lactis, while a mutation in the phage gene sav confers insensitivity to AbiV. In this study, we have further characterized the effects of the bacterial AbiV and its interaction with the phage p2 protein...

journal_title：Applied and environmental microbiology

authors： Haaber J,Samson JE,Labrie SJ,Campanacci V,Cambillau C,Moineau S,Hammer K

更新日期：2010-11-01 00:00:00

abstract：:A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head and a long noncontractile tail with a tail fiber. Genome sequencing revealed a double-stranded DNA of 59,254 bp having 73 open reading frames (ORFs). To identify the rece...

journal_title：Applied and environmental microbiology

authors： Choi Y,Shin H,Lee JH,Ryu S

更新日期：2013-08-01 00:00:00

abstract：:Two vomitoxin-producing isolates of Fusarium spp. were grown on cracked corn for 1 to 8 weeks at 15, 20, 25, 28, and 32 degrees C. Maximum production of vomitoxin by Fusarium graminearum Schw. NRRL 5883 occurred at 30 degrees C and 40 days, and that by Fusarium roseum Schw. NRRL 6101 occurred at 26 degrees C and 41 da...

journal_title：Applied and environmental microbiology

authors： Vesonder RF,Ellis JJ,Kwolek WF,DeMarini DJ

更新日期：1982-04-01 00:00:00

abstract：:Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are of...

journal_title：Applied and environmental microbiology

authors： Davey HM

更新日期：2011-08-15 00:00:00

abstract：:In this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in Brevibacterium aurantiacum. In agreement with the growth of B. aurantiacum in the presence of sulfate and c...

journal_title：Applied and environmental microbiology

authors： Forquin MP,Hébert A,Roux A,Aubert J,Proux C,Heilier JF,Landaud S,Junot C,Bonnarme P,Martin-Verstraete I

更新日期：2011-02-01 00:00:00

abstract：:The gray mold fungus Botrytis cinerea is a major threat to fruit and vegetable production. Strawberry fields usually receive several fungicide treatments against Botrytis per season. Gray mold isolates from several German strawberry-growing regions were analyzed to determine their sensitivity against botryticides. Fun...

journal_title：Applied and environmental microbiology

authors： Leroch M,Plesken C,Weber RW,Kauff F,Scalliet G,Hahn M

更新日期：2013-01-01 00:00:00

abstract：:Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were...

journal_title：Applied and environmental microbiology

authors： Klaassens ES,Boesten RJ,Haarman M,Knol J,Schuren FH,Vaughan EE,de Vos WM

更新日期：2009-05-01 00:00:00

abstract：:Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

journal_title：Applied and environmental microbiology

authors： Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

更新日期：1990-04-01 00:00:00

abstract：:CRISPR-based systems are emerging as the premier method to manipulate many cellular processes. In this study, a simple and efficient CRISPR interference (CRISPRi) system for targeted gene repression in archaea was developed. The Methanosarcina acetivorans CRISPR-Cas9 system was repurposed by replacing Cas9 with the ca...

journal_title：Applied and environmental microbiology

authors： Dhamad AE,Lessner DJ

更新日期：2020-10-15 00:00:00