Life, death, and in-between: meanings and methods in microbiology.

Abstract:

:Determination of microbial viability by the plate count method is routine in microbiology laboratories worldwide. However, limitations of the technique, particularly with respect to environmental microorganisms, are widely recognized. Many alternatives based upon viability staining have been proposed, and these are often combined with techniques such as image analysis and flow cytometry. The plethora of choices, however, adds to confusion when selecting a method. Commercial staining kits aim to simplify the performance of microbial viability determination but often still need adaptation to the specific organism of interest and/or the instruments available to the researcher. This review explores the meaning of microbial viability and offers guidance in the selection and interpretation of viability testing methods.

journal_name

Appl Environ Microbiol

authors

Davey HM

doi

10.1128/AEM.00744-11

subject

Has Abstract

pub_date

2011-08-15 00:00:00

pages

5571-6

issue

16

eissn

0099-2240

issn

1098-5336

pii

AEM.00744-11

journal_volume

77

pub_type

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