Abstract:
:The oxidation of methane in anoxic marine sediments is thought to be mediated by a consortium of methane-consuming archaea and sulfate-reducing bacteria. In this study, we compared results of rRNA gene (rDNA) surveys and lipid analyses of archaea and bacteria associated with methane seep sediments from several different sites on the Californian continental margin. Two distinct archaeal lineages (ANME-1 and ANME-2), peripherally related to the order Methanosarcinales, were consistently associated with methane seep marine sediments. The same sediments contained abundant (13)C-depleted archaeal lipids, indicating that one or both of these archaeal groups are members of anaerobic methane-oxidizing consortia. (13)C-depleted lipids and the signature 16S rDNAs for these archaeal groups were absent in nearby control sediments. Concurrent surveys of bacterial rDNAs revealed a predominance of delta-proteobacteria, in particular, close relatives of Desulfosarcina variabilis. Biomarker analyses of the same sediments showed bacterial fatty acids with strong (13)C depletion that are likely products of these sulfate-reducing bacteria. Consistent with these observations, whole-cell fluorescent in situ hybridization revealed aggregations of ANME-2 archaea and sulfate-reducing Desulfosarcina and Desulfococcus species. Additionally, the presence of abundant (13)C-depleted ether lipids, presumed to be of bacterial origin but unrelated to ether lipids of members of the order Desulfosarcinales, suggests the participation of additional bacterial groups in the methane-oxidizing process. Although the Desulfosarcinales and ANME-2 consortia appear to participate in the anaerobic oxidation of methane in marine sediments, our data suggest that other bacteria and archaea are also involved in methane oxidation in these environments.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Orphan VJ,Hinrichs KU,Ussler W 3rd,Paull CK,Taylor LT,Sylva SP,Hayes JM,Delong EFdoi
10.1128/AEM.67.4.1922-1934.2001keywords:
subject
Has Abstractpub_date
2001-04-01 00:00:00pages
1922-34issue
4eissn
0099-2240issn
1098-5336journal_volume
67pub_type
杂志文章abstract::Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with C...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.62.8.2883-2887.1996
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doi:10.1128/AEM.54.11.2660-2663.1988
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doi:10.1128/AEM.33.2.341-344.1977
更新日期:1977-02-01 00:00:00
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doi:10.1128/AEM.58.5.1524-1529.1992
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.6.1286-1291.1987
更新日期:1987-06-01 00:00:00
abstract::The bacterial communities associated with the development of necroses in injured agria cactus tissue were examined in the field by using both human-induced injuries and cactus tissue inoculated with cactophilic bacteria. Whole-cell bacterial fatty acids were used to determine when and where each of 23 detected species...
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更新日期:1994-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1990-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00