Comparison of the airborne survival of calf rotavirus and poliovirus type 1 (Sabin) aerosolized as a mixture.

abstract：:Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...

journal_title：Applied and environmental microbiology

authors： Kumagai T,Ozawa T,Tanimoto M,Noda M,Matoba Y,Sugiyama M

更新日期：2015-11-01 00:00:00

abstract：:Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that alpha/beta-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist ...

journal_title：Applied and environmental microbiology

authors： Paredes-Sabja D,Sarker N,Setlow B,Setlow P,Sarker MR

更新日期：2008-06-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:Resistance to HIV infection in a cohort of commercial sex workers living in Nairobi, Kenya, is linked to mucosal and antiinflammatory factors that may be influenced by the vaginal microbiota. Since bacterial vaginosis (BV), a polymicrobial dysbiosis characterized by low levels of protective Lactobacillus organisms, is...

journal_title：Applied and environmental microbiology

authors： Schellenberg JJ,Links MG,Hill JE,Dumonceaux TJ,Kimani J,Jaoko W,Wachihi C,Mungai JN,Peters GA,Tyler S,Graham M,Severini A,Fowke KR,Ball TB,Plummer FA

更新日期：2011-06-01 00:00:00

abstract：:This report describes the use of an oligonucleotide macroarray to profile the expression of 375 genes in Lactococcus lactis subsp. lactis IL1403 during heat, acid, and osmotic stress. A set of known stress-associated genes in IL1403 was used as the internal control on the array. Every stress response was accurately de...

journal_title：Applied and environmental microbiology

authors： Xie Y,Chou LS,Cutler A,Weimer B

更新日期：2004-11-01 00:00:00

abstract：:Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns a...

journal_title：Applied and environmental microbiology

authors： Mangin I,Bourget N,Bouhnik Y,Bisetti N,Simonet JM,Decaris B

更新日期：1994-05-01 00:00:00

abstract：:The deoxyribonucleic acid (DNA) yield and deoxyribonuclease (DNase) activity of several yeasts were correlated. Debaryomyces castellii and Debaryomyces franciscae were found to contain active DNases which carry out DNA hydrolysis, whereas the amounts of DNA as determined by extraction with Sarkosyl buffer (pH 7.8) wer...

journal_title：Applied and environmental microbiology

authors： El-Nakhal H,Phaff HJ

更新日期：1981-06-01 00:00:00

abstract：:The kinetics of adhesion of Vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. A modified Langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (Vibrio alginolyticus DP1HE4 and Vibrio anguillarum-like DC12R8 and DC12R9) showed a...

journal_title：Applied and environmental microbiology

authors： Bordas MA,Balebona MC,Zorrilla I,Borrego JJ,Moriñigo MA

更新日期：1996-10-01 00:00:00

abstract：:Despite the increasing knowledge of Synechococcus spp. and their co-occurring cyanophages in oceanic and coastal water, little is known about their abundance, distribution, and interactions in the Chesapeake Bay estuarine ecosystem. A 5-year interannual survey shows that Synechococcus spp. and their phages are persist...

journal_title：Applied and environmental microbiology

authors： Wang K,Wommack KE,Chen F

更新日期：2011-11-01 00:00:00

abstract：:An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

journal_title：Applied and environmental microbiology

authors： Coleman NV,Spain JC

更新日期：2003-10-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) causes serious foodborne disease worldwide. It produces the very potent Shiga toxin 2 (Stx2). The Stx2-encoding genes are located on a prophage, and production of the toxin is linked to the synthesis of Stx phages. There is, currently, no good treatment for EHEC infections, as...

journal_title：Applied and environmental microbiology

authors： Kijewski A,Witsø IL,Iversen H,Rønning HT,L'Abée-Lund T,Wasteson Y,Lindbäck T,Aspholm M

更新日期：2020-11-24 00:00:00

abstract：:PCR generated two distinct products from a toxic isolate of Alexandrium catenella, which had been taken from Dai Ya Bay (southern China), by using primers for large-subunit rRNA. This pattern is distinct from published data for North American Alexandrium species. Sequences of the two products suggest that the smaller ...

journal_title：Applied and environmental microbiology

authors： Yeung PK,Kong KF,Wong FT,Wong JT

更新日期：1996-11-01 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiologi...

journal_title：Applied and environmental microbiology

authors： Payne MS,Hall MR,Sly L,Bourne DG

更新日期：2007-03-01 00:00:00

abstract：:Metabolic inhibitors were added to a culture medium inoculated with theramlly stressed Vibrio parahaemolyticus to obtain information pertaining to biosynthetic processes required for recovery from heat damage. Ribonucleic acid and protein syntheses, in addition to membrane repair, were required during recovery of inju...

journal_title：Applied and environmental microbiology

authors： Heinis JJ,Beuchat LR,Boswell FC

更新日期：1978-06-01 00:00:00

abstract：:Thymidine incorporation by the microbial community on standing dead leaves of Spartina alterniflora did not obey many of the assumptions inherent in the use of the technique in planktonic systems. Incorporation rates of [methly-H]thymidine were nonsaturable over a wide concentration range (10 to 10 nM). Owing to metab...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Newell SY

更新日期：1986-11-01 00:00:00

abstract：:The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

journal_title：Applied and environmental microbiology

authors： Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

更新日期：2009-09-01 00:00:00

abstract：:Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were...

journal_title：Applied and environmental microbiology

authors： Johnson AD,Eddy GA,Gangemi JD,Ramsburg HH,Metzger JF

更新日期：1978-02-01 00:00:00

abstract：:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (...

journal_title：Applied and environmental microbiology

authors： Baleiras Couto MM,Eijsma B,Hofstra H,Huis in't Veld JH,van der Vossen JM

更新日期：1996-01-01 00:00:00

abstract：:The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...

journal_title：Applied and environmental microbiology

authors： Bebien M,Chauvin JP,Adriano JM,Grosse S,Verméglio A

更新日期：2001-10-01 00:00:00

abstract：:Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M Na...

journal_title：Applied and environmental microbiology

authors： Korber DR,Choi A,Wolfaardt GM,Caldwell DE

更新日期：1996-11-01 00:00:00

abstract：:The anoxygenic phototrophic bacterium Rhodopseudomonas palustris produces methane (CH4) from carbon dioxide (CO2) and hydrogen (H2) from protons (H+) when it expresses a variant form of molybdenum (Mo) nitrogenase that has two amino acid substitutions near its active site. We examined the influence of light energy and...

journal_title：Applied and environmental microbiology

authors： Zheng Y,Harwood CS

更新日期：2019-04-18 00:00:00

abstract：:Rhizopogon roseolus Corda (synonym Rhizopogon rubescens Tul.), an economically important edible mushroom associated with the Pinaceae (mostly Pinus sp.), has a global distribution resulting from the introduction of exotic trees into the Southern Hemisphere for plantation forestry. However, the marketability of R. rose...

journal_title：Applied and environmental microbiology

authors： Visnovsky SB,Guerin-Laguette A,Wang Y,Pitman AR

更新日期：2010-01-01 00:00:00

abstract：:A system of tangential-flow filtration was evaluated for use in the detection of Giardia cysts in drinking water. This method was more sensitive in recovering cysts than a frequently used wound-orlon system of through-filtration. ...

journal_title：Applied and environmental microbiology

authors： Isaac-Renton JL,Fung CP,Lochan A

更新日期：1986-08-01 00:00:00

abstract：:The culturability of three Campylobacter jejuni strains and their infectivity for day-old chicks were assessed following storage of the strains in saline. The potential for colonization of chicks was weakened during the storage period and terminated 3 to 4 weeks before the strains became nonculturable. The results fro...

journal_title：Applied and environmental microbiology

authors： Hald B,Knudsen K,Lind P,Madsen M

更新日期：2001-05-01 00:00:00

abstract：:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, req...

journal_title：Applied and environmental microbiology

authors： Yung PT,Ponce A

更新日期：2008-12-01 00:00:00

abstract：:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

journal_title：Applied and environmental microbiology

authors： Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

更新日期：2012-09-01 00:00:00