Comparison of genotypes and serotypes of Campylobacter jejuni isolated from Danish wild mammals and birds and from broiler flocks and humans.

abstract：:Presently, the only effective treatment for celiac disease is a life-long gluten-free diet. In this work, we used a new mixture of selected sourdough lactobacilli and fungal proteases to eliminate the toxicity of wheat flour during long-time fermentation. Immunological (R5 antibody-based sandwich and competitive enzym...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,De Angelis M,Di Cagno R,Camarca A,Silano M,Losito I,De Vincenzi M,De Bari MD,Palmisano F,Maurano F,Gianfrani C,Gobbetti M

更新日期：2007-07-01 00:00:00

abstract：:We investigated the in situ spatial organization of ammonia-oxidizing and nitrite-oxidizing bacteria in domestic wastewater biofilms and autotrophic nitrifying biofilms by using microsensors and fluorescent in situ hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes. The combination of these t...

journal_title：Applied and environmental microbiology

authors： Okabe S,Satoh H,Watanabe Y

更新日期：1999-07-01 00:00:00

abstract：:Mixed infections have important consequences for the ecology and evolution of host-parasite interactions. In vector-borne diseases, interactions between pathogens occur in both the vertebrate host and the arthropod vector. Spirochete bacteria belonging to the Borrelia burgdorferi sensu lato genospecies complex are tra...

journal_title：Applied and environmental microbiology

authors： Herrmann C,Gern L,Voordouw MJ

更新日期：2013-12-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00

abstract：:Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...

journal_title：Applied and environmental microbiology

authors： Johnson LJ,Miller AN,McCleery RA,McClanahan R,Kath JA,Lueschow S,Porras-Alfaro A

更新日期：2013-09-01 00:00:00

abstract：:Three common food pathogenic microorganisms were exposed to treatments simulating those used in food processing. Treated cell suspensions were then analyzed for reduction in growth by plate counting. Flow cytometry (FCM) and fluorescence-activated cell sorting (FACS) were carried out on treated cells stained for membr...

journal_title：Applied and environmental microbiology

authors： Kennedy D,Cronin UP,Wilkinson MG

更新日期：2011-07-01 00:00:00

abstract：:Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown ...

journal_title：Applied and environmental microbiology

authors： Suar M,van der Meer JR,Lawlor K,Holliger C,Lal R

更新日期：2004-11-01 00:00:00

abstract：:ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

journal_title：Applied and environmental microbiology

authors： Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

更新日期：2010-05-01 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:Resistance to Bacillus thuringiensis Cry1Ac toxin was characterized in a population of Helicoverpa zea larvae previously shown not to have an alteration in toxin binding as the primary resistance mechanism to this toxin. Cry1Ac-selected larvae (AR1) were resistant to protoxins and toxins of Cry1Ab, Cry1Ac, and the cor...

journal_title：Applied and environmental microbiology

authors： Caccia S,Moar WJ,Chandrashekhar J,Oppert C,Anilkumar KJ,Jurat-Fuentes JL,Ferré J

更新日期：2012-08-01 00:00:00

abstract：:Iron-oxidizing thiobacilli were adapted to grow on a chalcopyrite and a galena ore concentrate. When grown on the chalcopyrite concentrate, the bacteria exhibited a doubling time of 38.4 +/- 2.9 h, with a final cellular protein concentration of 185 mug/ml and solubilization of 10.3 g of copper per liter. When grown on...

journal_title：Applied and environmental microbiology

authors： Kingma JG,Silver M

更新日期：1980-03-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

journal_title：Applied and environmental microbiology

authors： Li XL,Chen H,Ljungdahl LG

更新日期：1997-02-01 00:00:00

abstract：:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

journal_title：Applied and environmental microbiology

authors： Sembries S,Crawford RL

更新日期：1997-05-01 00:00:00

abstract：:The oral biofilm community consists of >800 microbial species, among which Streptococcus mutans is considered a primary pathogen for dental caries. The genomic island TnSmu2 of S. mutans comprises >2% of the genome. In this study, we demonstrate that TnSmu2 harbors a gene cluster encoding nonribosomal peptide syntheta...

journal_title：Applied and environmental microbiology

authors： Wu C,Cichewicz R,Li Y,Liu J,Roe B,Ferretti J,Merritt J,Qi F

更新日期：2010-09-01 00:00:00

abstract：:The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

journal_title：Applied and environmental microbiology

authors： Oh HM,Lee SJ,Jang MH,Yoon BD

更新日期：2000-01-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...

journal_title：Applied and environmental microbiology

authors： Zdor RE,Pueppke SG

更新日期：1988-08-01 00:00:00

abstract：:The successful nodulation of legumes by a Rhizobium strain is determined by the competitive ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in a...

journal_title：Applied and environmental microbiology

authors： Moawad H,Bohlool BB

更新日期：1984-07-01 00:00:00

abstract：:The psychrotolerant acidophile Acidithiobacillus ferrivorans has been identified from cold environments and has been shown to use ferrous iron and inorganic sulfur compounds as its energy sources. A bioinformatic evaluation presented in this study suggested that Acidithiobacillus ferrivorans utilized a ferrous iron ox...

journal_title：Applied and environmental microbiology

authors： Liljeqvist M,Rzhepishevska OI,Dopson M

更新日期：2013-02-01 00:00:00

abstract：:This is the first report on the fate of enterohemorrhagic Escherichia coli O157:H7 in simulated human colonic conditions. The pathogen was progressively eliminated from the bioreactor and did not modify the major populations of resident microbiota. The coadministration of the Saccharomyces cerevisiae CNCM I-3856 probi...

journal_title：Applied and environmental microbiology

authors： Thévenot J,Etienne-Mesmin L,Denis S,Chalancon S,Alric M,Livrelli V,Blanquet-Diot S

更新日期：2013-02-01 00:00:00

abstract：:Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...

journal_title：Applied and environmental microbiology

authors： Ganeshnarayan K,Shah SM,Libera MR,Santostefano A,Kaplan JB

更新日期：2009-03-01 00:00:00

abstract：:The genus Bradyrhizobium has been considered to be a taxonomically difficult group. In this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among Bradyrhizobium strains nodulating soybeans in China. Eleven genospecies were identified by sequence analysis of three phyl...

journal_title：Applied and environmental microbiology

authors： Zhang XX,Guo HJ,Wang R,Sui XH,Zhang YM,Wang ET,Tian CF,Chen WX

更新日期：2014-05-01 00:00:00

abstract：:Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Brouwer-Hanzens AJ,Veenendaal HR,Wullings BA

更新日期：2016-10-27 00:00:00

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...

journal_title：Applied and environmental microbiology

authors： Dombek PE,Johnson LK,Zimmerley ST,Sadowsky MJ

更新日期：2000-06-01 00:00:00

abstract：:The structure and composition of the oocyst wall are primary factors determining the survival and hydrologic transport of Cryptosporidium parvum oocysts outside the host. Microscopic and biochemical analyses of whole oocysts and purified oocyst walls were undertaken to better understand the inactivation kinetics and h...

journal_title：Applied and environmental microbiology

authors： Jenkins MB,Eaglesham BS,Anthony LC,Kachlany SC,Bowman DD,Ghiorse WC

更新日期：2010-03-01 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

journal_title：Applied and environmental microbiology

authors： Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

更新日期：2015-07-01 00:00:00