Enterohemorrhagic Escherichia coli O157:H7 survival in an in vitro model of the human large intestine and interactions with probiotic yeasts and resident microbiota.

abstract：:Botulinum neurotoxin (BoNT) serotype B (BoNT/B) is one of the serotypes of BoNT that causes deadly human botulism, though it is used clinically for treatment of many neuromuscular diseases. BoNT/B is produced by Clostridium botulinum, and it is secreted along with a group of neurotoxin-associated proteins (NAPs) in th...

journal_title：Applied and environmental microbiology

authors： Wang HH,Riding S,Lindo P,Singh BR

更新日期：2010-10-01 00:00:00

abstract：:Four new primers and one published primer were used to PCR amplify hypervariable regions within the protozoal 18S rRNA gene to determine which primer pair provided the best identification and statistical analysis. PCR amplicons of 394 to 498 bases were generated from three primer sets, sequenced using Roche 454 pyrose...

journal_title：Applied and environmental microbiology

authors： Ishaq SL,Wright AD

更新日期：2014-09-01 00:00:00

abstract：:A method was developed to study the biodegradation and oxidative biodehalogenation of fluorinated phenols by 19F nuclear magnetic resonance (NMR). Characterization of the 19F NMR spectra of metabolite profiles of a series of fluorophenols, converted by purified phenol hydroxylase, catechol 1,2-dioxygenase, and/or by t...

journal_title：Applied and environmental microbiology

authors： Boersma MG,Dinarieva TY,Middelhoven WJ,van Berkel WJ,Doran J,Vervoort J,Rietjens IM

更新日期：1998-04-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:A novel sulfate-reducing bacterium isolated from fuel-contaminated subsurface soil, strain PRTOL1, mineralizes toluene as the sole electron donor and carbon source under strictly anaerobic conditions. The mineralization of 80% of toluene carbon to CO2 was demonstrated in experiments with [ring-U-14C]toluene; 15% of to...

journal_title：Applied and environmental microbiology

authors： Beller HR,Spormann AM,Sharma PK,Cole JR,Reinhard M

更新日期：1996-04-01 00:00:00

abstract：:Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...

journal_title：Applied and environmental microbiology

authors： Daisley BA,Trinder M,McDowell TW,Collins SL,Sumarah MW,Reid G

更新日期：2018-04-16 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00

abstract：:Sulfidogenic consortia enriched from an estuarine sediment were maintained on either 2-, 3-, or 4-chlorophenol as the only source of carbon and energy for over 5 years. The enrichment culture on 4-chlorophenol was the most active and this consortium was selected for further characterization. Utilization of chloropheno...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Young LY

更新日期：1995-04-01 00:00:00

abstract：:We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacteri...

journal_title：Applied and environmental microbiology

authors： Petersen KV,Martinussen J,Jensen PR,Solem C

更新日期：2013-06-01 00:00:00

abstract：:The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Esche...

journal_title：Applied and environmental microbiology

authors： Pfeifer BA,Wang CC,Walsh CT,Khosla C

更新日期：2003-11-01 00:00:00

abstract：:The ability of terminal restriction fragment (T-RFLP or TRF) profiles of 16S rRNA genes to provide useful information about the relative diversity of complex microbial communities was investigated by comparison with other methods. Four soil communities representing two pinyon rhizosphere and two between-tree (interspa...

journal_title：Applied and environmental microbiology

authors： Dunbar J,Ticknor LO,Kuske CR

更新日期：2000-07-01 00:00:00

abstract：:Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

journal_title：Applied and environmental microbiology

authors： Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

更新日期：2005-12-01 00:00:00

abstract：:The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cell...

journal_title：Applied and environmental microbiology

authors： Bendinger B,Rijnaarts HH,Altendorf K,Zehnder AJ

更新日期：1993-11-01 00:00:00

abstract：:The diversity of deep-sea high-pressure-adapted (piezophilic) microbes in isolated monoculture remains low. In this study, a novel obligately psychropiezophilic bacterium was isolated from seawater collected from the Puerto Rico Trench at a depth of ∼6,000 m. This isolate, designated YC-1, grew best in a nutrient-rich...

journal_title：Applied and environmental microbiology

authors： Cao Y,Chastain RA,Eloe EA,Nogi Y,Kato C,Bartlett DH

更新日期：2014-01-01 00:00:00

abstract：:A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

journal_title：Applied and environmental microbiology

authors： Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

更新日期：2006-10-01 00:00:00

abstract：:Bacterial signaling is an important part of community life, but little is known about the signal transduction pathways of the as-yet-uncultured members of microbial communities. To address this gap, we aimed to identify genes directing the synthesis of signals in uncultured bacteria associated with the midguts of gyps...

journal_title：Applied and environmental microbiology

authors： Guan C,Ju J,Borlee BR,Williamson LL,Shen B,Raffa KF,Handelsman J

更新日期：2007-06-01 00:00:00

abstract：:Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typi...

journal_title：Applied and environmental microbiology

authors： Varshney AK,Mediavilla JR,Robiou N,Guh A,Wang X,Gialanella P,Levi MH,Kreiswirth BN,Fries BC

更新日期：2009-11-01 00:00:00

abstract：:Receptors on the cell surfaces of bacterial hosts are essential during the infection cycle of bacteriophages. To date, the phage receptors of the industrial relevant dairy starter bacterium Streptococcus thermophilus remain elusive. Thus, we set out to identify cell surface structures that are involved in host recogni...

journal_title：Applied and environmental microbiology

authors： Szymczak P,Filipe SR,Covas G,Vogensen FK,Neves AR,Janzen T

更新日期：2018-11-15 00:00:00

abstract：:This study describes the genotypic characteristics of a collection of 100 multidrug-resistant (MDR) Escherichia coli strains recovered from cattle and the farm environment in Ireland in 2007. The most prevalent antimicrobial resistance identified was to streptomycin (100%), followed by tetracycline (99%), sulfonamides...

journal_title：Applied and environmental microbiology

authors： Karczmarczyk M,Walsh C,Slowey R,Leonard N,Fanning S

更新日期：2011-10-01 00:00:00

abstract：:Combined analysis of 5,179 serial phage reactions of 20 Listeria monocytogenes propagating strains over 14 years and phage typing results from 2,659 further L. monocytogenes strains allowed us to estimate lytic spectrum specificity and the variability of the lytic reactions of 35 phages. These included the 26 phages r...

journal_title：Applied and environmental microbiology

authors： Marquet-Van der Mee N,Audurier A

更新日期：1995-01-01 00:00:00

abstract：:Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

journal_title：Applied and environmental microbiology

authors： Zhang L,Sun C

更新日期：2018-08-31 00:00:00

abstract：:The influence of physiological and environmental factors on the accumulation of oxindole during anaerobic indole metabolism was investigated by high-performance liquid chromatography. Under methanogenic conditions, indole was temporarily converted to oxindole in stoichiometric amounts in media inoculated with three fr...

journal_title：Applied and environmental microbiology

authors： Madsen EL,Francis AJ,Bollag JM

更新日期：1988-01-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:An organism capable of growth on pyridine was isolated from soil by enrichment culture techniques and identified as Micrococcus luteus. The organism oxidized pyridine for energy and released N contained in the pyridine ring as ammonium. The organism could not grow on mono- or disubstituted pyridinecarboxylic acids or ...

journal_title：Applied and environmental microbiology

authors： Sims GK,Sommers LE,Konopka A

更新日期：1986-05-01 00:00:00

abstract：:Of some 350 microorganisms screened, four strains of Pithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol: Pithomyces sp. NRJ201, P. chartarum NRJ210, and, to a lesser extent, P. cynodontis ATCC 26150 and P. atro-olivaceus IFO 6651 were found ...

journal_title：Applied and environmental microbiology

authors： Suhara Y,Itoh S,Ogawa M,Yokose K,Sawada T,Sano T,Ninomiya R,Maruyama HB

更新日期：1981-08-01 00:00:00

abstract：:A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glyci...

journal_title：Applied and environmental microbiology

authors： Juni E,Heym GA,Avery M

更新日期：1986-09-01 00:00:00

abstract：:We determined the prevalences of Escherichia coli O157:H7 in feces, hide, and carcasses of meat goats at a U.S. processing plant. Prevalences were 11.1%, 2.7%, and 2.7%, respectively. Sixteen pulsed-field gel electrophoresis (PFGE) subtypes were identified among 49 E. coli O157:H7 isolates, some of which were present ...

journal_title：Applied and environmental microbiology

authors： Jacob ME,Foster DM,Rogers AT,Balcomb CC,Sanderson MW

更新日期：2013-07-01 00:00:00

abstract：:Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. ...

journal_title：Applied and environmental microbiology

authors： Heller D,Helmerhorst EJ,Gower AC,Siqueira WL,Paster BJ,Oppenheim FG

更新日期：2016-01-08 00:00:00

abstract：:An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum L...

journal_title：Applied and environmental microbiology

authors： Fitzsimons A,Hols P,Jore J,Leer RJ,O'Connell M,Delcour J

更新日期：1994-10-01 00:00:00

abstract：:The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...

journal_title：Applied and environmental microbiology

authors： Urakawa H,Tajima Y,Numata Y,Tsuneda S

更新日期：2008-02-01 00:00:00