Regio-selective 10-hydroxylation of patchoulol, a sesquiterpene, by pithomyces species.


:Of some 350 microorganisms screened, four strains of Pithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol: Pithomyces sp. NRJ201, P. chartarum NRJ210, and, to a lesser extent, P. cynodontis ATCC 26150 and P. atro-olivaceus IFO 6651 were found to catalyze this reaction. A method has been developed by which 10-hydroxypatchoulol was obtained in 25 to 45% yields in 1- to 5-liter fermentation jars at 2 to 4 g of patchoulol per liter and isolated as pure material in 30% yields.


Appl Environ Microbiol


Suhara Y,Itoh S,Ogawa M,Yokose K,Sawada T,Sano T,Ninomiya R,Maruyama HB





Has Abstract


1981-08-01 00:00:00












  • Outermost-cell-surface changes in an encapsulated strain of Staphylococcus aureus after preservation by freeze-drying.

    abstract::The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ohtomo T,Yamada T,Yoshida K

    更新日期:1988-10-01 00:00:00

  • Transformation of Dibenzo-p-Dioxin by Pseudomonas sp. Strain HH69.

    abstract::Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

    更新日期:1990-04-01 00:00:00

  • Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers.

    abstract::A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Herman LM,De Block JH,Moermans RJ

    更新日期:1995-02-01 00:00:00

  • Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-alpha-Pyrone Biosynthesis by Trichoderma Species.

    abstract::The understanding of the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-C]linoleic acid or [5-C]sodium mevalonate revealed that both fungal strains...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Serrano-Carreon L,Hathout Y,Bensoussan M,Belin JM

    更新日期:1993-09-01 00:00:00

  • Chemical and Physical Characterization of Interfacial-Active Lipids from Rhodococcus erythropolis Grown on n-Alkanes.

    abstract::Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kretschmer A,Bock H,Wagner F

    更新日期:1982-10-01 00:00:00

  • Nonnodulating Bradyrhizobium spp. Modulate the Benefits of Legume-Rhizobium Mutualism.

    abstract:UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

    更新日期:2016-08-15 00:00:00

  • The bactericidal activity of pediocin PA-1 is specifically inhibited by a 15-mer fragment that spans the bacteriocin from the center toward the C terminus.

    abstract::A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

    更新日期:1998-12-01 00:00:00

  • Inhibitor Evaluation with Immobilized Nitrobacter agilis Cells.

    abstract::Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tsai YL,Schlasner SM,Tuovinen OH

    更新日期:1986-12-01 00:00:00

  • Nucleotide sequence and expression of a novel pectate lyase gene (pel-3) and a closely linked endopolygalacturonase gene (peh-1) of Erwinia carotovora subsp. carotovora 71.

    abstract::Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Liu Y,Chatterjee A,Chatterjee AK

    更新日期:1994-07-01 00:00:00

  • Assessment of soil microbial community structure by use of taxon-specific quantitative PCR assays.

    abstract::Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Fierer N,Jackson JA,Vilgalys R,Jackson RB

    更新日期:2005-07-01 00:00:00

  • Extracellular Polysaccharide Is Not Responsible for Aluminum Tolerance of Rhizobium leguminosarum bv. Phaseoli CIAT899.

    abstract::Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Kingsley MT,Bohlool BB

    更新日期:1992-04-01 00:00:00

  • Effect of chlorine dioxide gas on fungi and mycotoxins associated with sick building syndrome.

    abstract::The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

    更新日期:2005-09-01 00:00:00

  • Comprehensive census of bacteria in clean rooms by using DNA microarray and cloning methods.

    abstract::A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

    更新日期:2009-10-01 00:00:00

  • Molecular cloning, purification, and biochemical characterization of hydantoin racemase from the legume symbiont Sinorhizobium meliloti CECT 4114.

    abstract::Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

    更新日期:2004-01-01 00:00:00

  • Role of leaf surface sugars in colonization of plants by bacterial epiphytes.

    abstract::The relationship between nutrients leached onto the leaf surface and the colonization of plants by bacteria was studied by measuring both the abundance of simple sugars and the growth of Pseudomonas fluorescens on individual bean leaves. Data obtained in this study indicate that the population size of epiphytic bacter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mercier J,Lindow SE

    更新日期:2000-01-01 00:00:00

  • Salmonella and Escherichia coli O157:H7 survival in soil and translocation into leeks (Allium porrum) as influenced by an arbuscular mycorrhizal fungus (Glomus intraradices).

    abstract::A study was conducted to determine the influence of arbuscular mycorrhizal (AM) fungi on Salmonella and enterohemorrhagic Escherichia coli O157:H7 (EHEC) in autoclaved soil and translocation into leek plants. Six-week-old leek plants (with [Myc+] or without [Myc-] AM fungi) were inoculated with composite suspensions o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Gurtler JB,Douds DD Jr,Dirks BP,Quinlan JJ,Nicholson AM,Phillips JG,Niemira BA

    更新日期:2013-03-01 00:00:00

  • Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia.

    abstract::Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hewson I,Fuhrman JA

    更新日期:2004-06-01 00:00:00

  • Lipidomics of Thalassiosira pseudonana under Phosphorus Stress Reveal Underlying Phospholipid Substitution Dynamics and Novel Diglycosylceramide Substitutes.

    abstract::Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

    更新日期:2018-03-01 00:00:00

  • Spoilage association of chicken leg muscle.

    abstract::The ability of pure cultures of bacteria isolated from spoiling chicken leg muscle to produce strong off-odors was tested by using sterile leg muscle sections. Changes in the flora during storage and the incidence and identity of organisms capable of producing strong off-odors were noted. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: McMeekin TA

    更新日期:1977-06-01 00:00:00

  • Production of penicillic acid and patulin by an atypical Penicillium roqueforti isolate.

    abstract::Simultaneous production of penicillic acid and patulin by an atypical strain of Penicillium roqueforti isolated from cheddar cheese is reported. Mycotoxin production was confirmed by thin-layer and gas-liquid chromatography and by ultraviolet, infrared, and mass spectral analyses. Culture extracts were toxic to Bacill...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Olivigni FJ,Bullerman LB

    更新日期:1978-02-01 00:00:00

  • Rhizonin, the first mycotoxin isolated from the zygomycota, is not a fungal metabolite but is produced by bacterial endosymbionts.

    abstract::Rhizonin is a hepatotoxic cyclopeptide isolated from cultures of a fungal Rhizopus microsporus strain that grew on moldy ground nuts in Mozambique. Reinvestigation of this fungal strain by a series of experiments unequivocally revealed that this "first mycotoxin from lower fungi" is actually not produced by the fungus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Partida-Martinez LP,de Looss CF,Ishida K,Ishida M,Roth M,Buder K,Hertweck C

    更新日期:2007-02-01 00:00:00

  • Effect of light on the cell cycle of a marine synechococcus strain.

    abstract::Light-dependent regulation of cell cycle progression in the marine cyanobacterium Synechococcus strain WH-8101 was demonstrated through the use of flow cytometry. Our results show that, similar to eucaryotic cells, marine Synechococcus spp. display two gaps in DNA synthesis, at the beginning and at the end of the cell...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Armbrust EV,Bowen JD,Olson RJ,Chisholm SW

    更新日期:1989-02-01 00:00:00

  • Coastal strains of marine Synechococcus species exhibit increased tolerance to copper shock and a distinctive transcriptional response relative to those of open-ocean strains.

    abstract::Copper appears to be influencing the distribution and abundance of phytoplankton in marine environments, and cyanobacteria are thought to be the most sensitive of the phytoplankton groups to copper toxicity. By using growth assays of phylogenetically divergent clades, we found that coastal strains of marine Synechococ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Stuart RK,Dupont CL,Johnson DA,Paulsen IT,Palenik B

    更新日期:2009-08-01 00:00:00

  • Cooccurrence of ScDSP gene expression, cell death, and DNA fragmentation in a marine diatom, Skeletonema costatum.

    abstract::A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Chung CC,Hwang SP,Chang J

    更新日期:2005-12-01 00:00:00

  • Relative Abundance of Nitrotoga spp. in a Biofilter of a Cold-Freshwater Aquaculture Plant Appears To Be Stimulated by Slightly Acidic pH.

    abstract::The functioning of recirculation aquaculture systems (RAS) is essential to maintain water quality for fish health, and one crucial process here is nitrification. The investigated RAS was connected to a rainbow trout production system and operated at an average temperature of 13°C and pH 6.8. Community analyses of the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Hüpeden J,Wegen S,Off S,Lücker S,Bedarf Y,Daims H,Kühn C,Spieck E

    更新日期:2016-01-08 00:00:00

  • Submicronic fungal bioaerosols: high-resolution microscopic characterization and quantification.

    abstract::Submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. In vitro generation of these particles has been studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments by size and discrim...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Afanou KA,Straumfors A,Skogstad A,Nilsen T,Synnes O,Skaar I,Hjeljord L,Tronsmo A,Green BJ,Eduard W

    更新日期:2014-11-01 00:00:00

  • Biosynthesis of Yersiniabactin, a complex polyketide-nonribosomal peptide, using Escherichia coli as a heterologous host.

    abstract::The medicinal value associated with complex polyketide and nonribosomal peptide natural products has prompted biosynthetic schemes dependent upon heterologous microbial hosts. Here we report the successful biosynthesis of yersiniabactin (Ybt), a model polyketide-nonribosomal peptide hybrid natural product, using Esche...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pfeifer BA,Wang CC,Walsh CT,Khosla C

    更新日期:2003-11-01 00:00:00

  • Expanded Fermi solution for estimating the survival of ingested pathogenic and probiotic microbial cells and spores.

    abstract::The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Peleg M,Normand MD,Horowitz J,Corradini MG

    更新日期:2011-01-01 00:00:00

  • The Hybrid Pre-CTXΦ-RS1 Prophage Genome and Its Regulatory Function in Environmental Vibrio cholerae O1 Strains.

    abstract::The cholera toxin genes of Vibrio cholerae are encoded by CTXΦ, a lysogenic bacteriophage. Infection with this phage plays a determinant role in toxigenicity conversion and the emergence of new clones of pathogenic V. cholerae. Multiple phage alleles, defined by sequence types of the repressor gene rstR, have been fou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Wang H,Pang B,Xiong L,Wang D,Wang X,Zhang L,Kan B

    更新日期:2015-10-01 00:00:00

  • Rapid extraction of plasmids from Clostridium perfringens.

    abstract::Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

    更新日期:1986-03-01 00:00:00