Impact of inoculation protocols, salinity, and pH on the degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of PAH-degrading bacteria introduced into soil.

abstract：:The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

journal_title：Applied and environmental microbiology

authors： Nies L,Vogel TM

更新日期：1990-09-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentative...

journal_title：Applied and environmental microbiology

authors： Taller BJ,Wong TY

更新日期：1989-01-01 00:00:00

abstract：:The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled wi...

journal_title：Applied and environmental microbiology

authors： Mullane NR,Ryan M,Iversen C,Murphy M,O'Gaora P,Quinn T,Whyte P,Wall PG,Fanning S

更新日期：2008-02-01 00:00:00

abstract：:Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobac...

journal_title：Applied and environmental microbiology

authors： Inoue K,Usami Y,Ashikawa Y,Noguchi H,Umeda T,Yamagami-Ashikawa A,Horisaki T,Uchimura H,Terada T,Nakamura S,Shimizu K,Habe H,Yamane H,Fujimoto Z,Nojiri H

更新日期：2014-05-01 00:00:00

abstract：:Pure cultures of Escherichia coli and Streptococcus faecalis and environmental water samples were examined for the possibility that pollution involving organotin compounds could decrease the values for indicator organisms when standard methods were applied to the analysis of water samples. (CH3)2SnCl2 and (CH3)3SnCl d...

journal_title：Applied and environmental microbiology

authors： Pettibone GW,Cooney JJ

更新日期：1986-09-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00

abstract：:Deep-subsurface samples obtained by deep drilling are likely to be contaminated with mesophilic microorganisms in the drilling fluid, and this could affect determination of the community structure of the geothermal microflora using 16S rRNA gene clone library analysis. To eliminate possible contamination by PCR-amplif...

journal_title：Applied and environmental microbiology

authors： Kimura H,Sugihara M,Kato K,Hanada S

更新日期：2006-01-01 00:00:00

abstract：:The kinetics of the enzymatic formation of 7,8-epoxy-1-octene, 1,2-7,8-diepoxyoctane, and 1,2-epoxyoctane by growing and resting cell suspensions of Pseudomonas oleovorans are described. Formation of 1,2-epoxyoctane occurs concurrently with exponential growth on 1-octene, providing that 1-octene is in excess. Conversi...

journal_title：Applied and environmental microbiology

authors： Schwartz RD,McCoy CJ

更新日期：1976-01-01 00:00:00

abstract：:Poultry are considered the major reservoir for Campylobacter jejuni, a leading bacterial cause of human food-borne diarrhea. To understand the ecology of C. jejuni and develop strategies to control C. jejuni infection in the animal reservoir, we initiated studies to examine the potential role of anti-Campylobacter mat...

journal_title：Applied and environmental microbiology

authors： Sahin O,Zhang Q,Meitzler JC,Harr BS,Morishita TY,Mohan R

更新日期：2001-09-01 00:00:00

abstract：:The effect of condensed tannins from birdsfoot trefoil (Lotus corniculatus L.) on the cellulolytic rumen bacterium Fibrobacter succinogenes S85 was examined. Condensed tannins inhibited endoglucanase activity in the extracellular culture fluid, at concentrations as low as 25 mug ml. In contrast, cell-associated endogl...

journal_title：Applied and environmental microbiology

authors： Bae HD,McAllister TA,Yanke J,Cheng KJ,Muir AD

更新日期：1993-07-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:Data from analyses of continuous culture fermentation of insoluble cellulose by Ruminococcus albus 7 were used to derive constants for the rate of cellulose hydrolysis and fermentation, growth yield, and maintenance. Cellulose concentration was 1% in the nutrient reservoir, and hydraulic retention times of 0.5, 1.0, 1...

journal_title：Applied and environmental microbiology

authors： Pavlostathis SG,Miller TL,Wolin MJ

更新日期：1988-11-01 00:00:00

abstract：:α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

journal_title：Applied and environmental microbiology

authors： Nikitkova AE,Haase EM,Scannapieco FA

更新日期：2013-01-01 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose cont...

journal_title：Applied and environmental microbiology

authors： Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

更新日期：2011-08-01 00:00:00

abstract：:Ammoniacal silver nitrate (10 mg/ml) was added to terminate acetylene reduction assays used to measure nitrogenase activity. Silver nitrate quantitatively precipitated acetylene as the carbide salt, but did not affect the ethylene formed. The vials containing ethylene can be analyzed gas chromatographically at the rat...

journal_title：Applied and environmental microbiology

authors： David KA,Apte SK,Banerji A,Thomas J

更新日期：1980-05-01 00:00:00

abstract：:Microbial partners play important roles in the biology and ecology of animals. In insects, maternally transmitted symbionts are especially common and can have host effects ranging from reproductive manipulation to nutrient provisioning and defense against natural enemies. In this study, we report a genus-wide associat...

journal_title：Applied and environmental microbiology

authors： Ballinger MJ,Moore LD,Perlman SJ

更新日期：2018-01-31 00:00:00

abstract：:The use of two semisolid media (semisolid Rappaport and semisolid Rappaport-Vassiliadis) for the isolation of Salmonella spp. from coastal waters was compared with the use of conventional media. Two hundred and fifty six samples were studied; Salmonella spp. were detected in 83. The semisolid media were the most sensi...

journal_title：Applied and environmental microbiology

authors： Perales I,Audicana A

更新日期：1989-11-01 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:Seven different sources of inocula that included sediments, contaminated soils, groundwater, process effluent, and sludge were used to establish enrichment cultures of denitrifying bacteria on benzene, toluene, and xylenes in the absence of molecular oxygen. All of the enrichment cultures demonstrated complete depleti...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Mang DT,Young LY

更新日期：1991-02-01 00:00:00

abstract：:A major taxon of obligate marine bacteria within the order Actinomycetales has been discovered from ocean sediments. Populations of these bacteria (designated MAR 1) are persistent and widespread, spanning at least three distinct ocean systems. In this study, 212 actinomycete isolates possessing MAR 1 morphologies wer...

journal_title：Applied and environmental microbiology

authors： Mincer TJ,Jensen PR,Kauffman CA,Fenical W

更新日期：2002-10-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...

journal_title：Applied and environmental microbiology

authors： Bebien M,Chauvin JP,Adriano JM,Grosse S,Verméglio A

更新日期：2001-10-01 00:00:00

abstract：:A total of 896 samples of retail fresh meats and poultry was assayed for Escherichia coli serogroup O157:H7 by a hydrophobic grid membrane filter-immunoblot procedure developed specifically to isolate the organism from foods. The procedure involves several steps, including selective enrichment, filtration of enrichmen...

journal_title：Applied and environmental microbiology

authors： Doyle MP,Schoeni JL

更新日期：1987-10-01 00:00:00

abstract：:Sclerotinia sclerotiorum acidifies its ambient environment by producing oxalic acid. This production of oxalic acid during plant infection has been implicated as a primary determinant of pathogenicity in this and other phytopathogenic fungi. We found that ambient pH conditions affect multiple processes in S. sclerotio...

journal_title：Applied and environmental microbiology

authors： Rollins JA,Dickman MB

更新日期：2001-01-01 00:00:00

abstract：:Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

journal_title：Applied and environmental microbiology

authors： Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

更新日期：2014-01-01 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:Pep5 is a cationic pore-forming lantibiotic produced by Staphylococcus epidermidis strain 5. The producer strain protects itself from the lethal action of its own bacteriocin through the 69-amino-acid immunity peptide PepI. The N-terminal segment of PepI contains a 20-amino-acid stretch of apolar residues, whereas the...

journal_title：Applied and environmental microbiology

authors： Hoffmann A,Schneider T,Pag U,Sahl HG

更新日期：2004-06-01 00:00:00