Kinetics of Insoluble Cellulose Fermentation by Continuous Cultures of Ruminococcus albus.

abstract：:RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, a...

journal_title：Applied and environmental microbiology

authors： Kamekura M,Hamakawa T,Onishi H

更新日期：1982-10-01 00:00:00

abstract：:Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...

journal_title：Applied and environmental microbiology

authors： McMillan DG,Keis S,Berney M,Cook GM

更新日期：2009-12-01 00:00:00

abstract：:In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Amaro C

更新日期：1996-07-01 00:00:00

abstract：:Pyruvate carboxylase (PYC) is an ecologically, medically, and industrially important enzyme. It is widespread in all three domains of life, the archaea, bacteria, and eukarya. PYC catalyzes ATP-dependent carboxylation of pyruvate to oxaloacetate. Detailed structure-function studies of this enzyme have been hampered du...

journal_title：Applied and environmental microbiology

authors： Lai H,Kraszewski JL,Purwantini E,Mukhopadhyay B

更新日期：2006-12-01 00:00:00

abstract：:Oxidation of acetate in salt marsh sediment was inhibited by the addition of fluoroacetate, and also by the addition of molybdate, an inhibitor of sulfate-reducing bacteria. Molybdate had no effect upon the metabolism of acetate in a freshwater sediment in the absence of sulfate. The inhibitory effect of molybdate on ...

journal_title：Applied and environmental microbiology

authors： Banat IM,Lindström EB,Nedwell DB,Balba MT

更新日期：1981-12-01 00:00:00

abstract：:Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (<0.9 muM) were required for germination. Nitrate had no effect, but ammonia, at concentrations of >45 muM, inhibited germination. Salinities of >20 pe...

journal_title：Applied and environmental microbiology

authors： Huber AL

更新日期：1985-01-01 00:00:00

abstract：:Different Rhizobium and Bradyrhizobium strains were screened for their ability to produce melanin. Pigment producers (Mel) were found among strains of R. leguminosarum biovars viceae, trifolii, and phaseoli, R. meliloti, and R. fredii; none of 19 Bradyrhizobium strains examined gave a positive response. Melanin produc...

journal_title：Applied and environmental microbiology

authors： Cubo MT,Buendia-Claveria AM,Beringer JE,Ruiz-Sainz JE

更新日期：1988-07-01 00:00:00

abstract：:There have been few reports on the epidemiological analysis of environmental Leptospira isolates. This is probably because the isolation of leptospires from the environment was usually unsuccessful due to the overgrowth of contaminants and the slow growth of Leptospira. In this study, we collected a total of 88 sample...

journal_title：Applied and environmental microbiology

authors： Saito M,Villanueva SY,Chakraborty A,Miyahara S,Segawa T,Asoh T,Ozuru R,Gloriani NG,Yanagihara Y,Yoshida S

更新日期：2013-01-01 00:00:00

abstract：:Two genes involved in aflatoxin B1 (AFB1) biosynthesis in Aspergillus parasiticus, nor-1 and ver-1, were localized to a 35-kb region on one A. parasiticus chromosome and to the genomic DNA fragment carried on a single cosmid, NorA. A physical and transcriptional map of the 35-kb genomic DNA insert in cosmid NorA was p...

journal_title：Applied and environmental microbiology

authors： Trail F,Mahanti N,Rarick M,Mehigh R,Liang SH,Zhou R,Linz JE

更新日期：1995-07-01 00:00:00

abstract：:Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocus...

journal_title：Applied and environmental microbiology

authors： Goller SP,Schoisswohl D,Baron M,Parriche M,Kubicek CP

更新日期：1998-09-01 00:00:00

abstract：:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation mi...

journal_title：Applied and environmental microbiology

authors： Rizzi A,Leroux J,Charron-Lamoureux V,Roy S,Beauregard PB,Bellenger JP

更新日期：2020-10-28 00:00:00

abstract：:To assess the relative merits of tryptone yeast extract agar, the same medium unbuffered, and medium M17 for the assay of nine bacteriophages of lactic streptococci, comparative plaque counts were made with an overlay of 3 or 9 ml. Four of the phages exhibited no significant difference in plating efficiency between me...

journal_title：Applied and environmental microbiology

authors： Keogh BP

更新日期：1980-10-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and m...

journal_title：Applied and environmental microbiology

authors： Mestrom L,Marsden SR,Dieters M,Achterberg P,Stolk L,Bento I,Hanefeld U,Hagedoorn PL

更新日期：2019-04-04 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:Evaluation of the fate and transport of biological warfare (BW) agents in landfills requires the development of specific and sensitive detection assays. The objective of the current study was to develop and validate SYBR green quantitative real-time PCR (Q-PCR) assays for the specific detection and quantification of s...

journal_title：Applied and environmental microbiology

authors： Saikaly PE,Barlaz MA,de Los Reyes FL 3rd

更新日期：2007-10-01 00:00:00

abstract：:Kasugamycin (KSM), a unique aminoglycoside antibiotic, has been used in agriculture for many years to control not only rice blast caused by the fungus Magnaporthe grisea but also rice bacterial grain and seedling rot or rice bacterial brown stripe caused by Burkholderia glumae or Acidovorax avenae subsp. avenae, respe...

journal_title：Applied and environmental microbiology

authors： Yoshii A,Moriyama H,Fukuhara T

更新日期：2012-08-01 00:00:00

abstract：:The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

journal_title：Applied and environmental microbiology

authors： Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

更新日期：1996-06-01 00:00:00

abstract：:The thermophilic homoacetogenic bacterium Moorella sp. strain HUC22-1 ferments glyoxylate to acetate roughly according to the reaction 2 glyoxylate --> acetate + 2 CO(2). A batch culture with glyoxylate and yeast extract yielded 11.7 g per mol of cells per substrate, which was much higher than that obtained with H(2) ...

journal_title：Applied and environmental microbiology

authors： Sakai S,Inokuma K,Nakashimada Y,Nishio N

更新日期：2008-03-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:The ferric uptake regulator (Fur) box-like sequence was located upstream of the serine protease-encoding gene (aprII) from a marine bacterium, Alteromonas sp. strain O-7. To clarify whether the production of AprII (the gene product of aprII) is regulated by the environmental iron concentrations, this strain was cultur...

journal_title：Applied and environmental microbiology

authors： Tsujibo H,Miyamoto K,Okamoto T,Orikoshi H,Inamori Y

更新日期：2000-09-01 00:00:00

abstract：:The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

journal_title：Applied and environmental microbiology

authors： Tucker AM,Driskell LO,Pannell LK,Wood DO

更新日期：2011-07-01 00:00:00

abstract：:Acid-adapted strains of Escherichia coli K-12 W3110 were obtained by serial culture in medium buffered at pH 4.6 (M. M. Harden, A. He, K. Creamer, M. W. Clark, I. Hamdallah, K. A. Martinez, R. L. Kresslein, S. P. Bush, and J. L. Slonczewski, Appl Environ Microbiol 81:1932-1941, 2015, https://doi.org/10.1128/AEM.03494-...

journal_title：Applied and environmental microbiology

authors： He A,Penix SR,Basting PJ,Griffith JM,Creamer KE,Camperchioli D,Clark MW,Gonzales AS,Chávez Erazo JS,George NS,Bhagwat AA,Slonczewski JL

更新日期：2017-05-31 00:00:00

abstract：:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by...

journal_title：Applied and environmental microbiology

authors： Klijn N,Weerkamp AH,de Vos WM

更新日期：1995-07-01 00:00:00

abstract：:Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...

journal_title：Applied and environmental microbiology

authors： Krzeslak J,Gerritse G,van Merkerk R,Cool RH,Quax WJ

更新日期：2008-03-01 00:00:00

abstract：:The degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in Rhodococcus sp. strain TE1. Plasmid DNA libraries of Rhodococcus sp. strain TE1 were constructed in a Rhodococcus-Escherichia coli shu...

journal_title：Applied and environmental microbiology

authors： Shao ZQ,Behki R

更新日期：1995-05-01 00:00:00

abstract：:Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and phea...

journal_title：Applied and environmental microbiology

authors： Kovanen S,Rossi M,Pohja-Mykrä M,Nieminen T,Raunio-Saarnisto M,Sauvala M,Fredriksson-Ahomaa M,Hänninen ML,Kivistö R

更新日期：2019-02-06 00:00:00

abstract：:Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were...

journal_title：Applied and environmental microbiology

authors： Klaassens ES,Boesten RJ,Haarman M,Knol J,Schuren FH,Vaughan EE,de Vos WM

更新日期：2009-05-01 00:00:00

abstract：:Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...

journal_title：Applied and environmental microbiology

authors： Fuentes FA,Biamon EJ,Hazen TC

更新日期：1983-12-01 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00