Abstract:
:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by molecular detection techniques based on specific PCR amplification of the nisin (nisA) gene from DNA extracted from human feces. A method was developed for the efficient extraction of microbial DNA from human feces. The results show that a fraction of viable cells of L. lactis TC165.5 survived passage through the human gastrointestinal tract. Only cells that passed within 3 days of ingestion could be recovered from the feces of the volunteers, and they accounted for approximately 1% of the total number of cells consumed. The presence of nisA in DNA extracted from feces could be detected up to 4 days, when viable cells were no longer present.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Klijn N,Weerkamp AH,de Vos WMdoi
10.1128/AEM.61.7.2771-2774.1995subject
Has Abstractpub_date
1995-07-01 00:00:00pages
2771-4issue
7eissn
0099-2240issn
1098-5336journal_volume
61pub_type
杂志文章abstract::The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.12.4605-4607.1994
更新日期:1994-12-01 00:00:00
abstract::Cells of Bacillus subtilis, when suspended in a 5mM metal solution, bind metals tenaciously to their cell walls. These metal-loaded cells, when mixed with a synthetic sediment and put under laboratory conditions to simulate low-temperature sediment diagenesis, nucleate the formation of a mixed assemblage of crystallin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.3.1094-1108.1983
更新日期:1983-03-01 00:00:00
abstract::Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.11.6545-6553.2005
更新日期:2005-11-01 00:00:00
abstract::Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.4.1335-1341.1994
更新日期:1994-04-01 00:00:00
abstract::Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the results can provide inacc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00592-14
更新日期:2014-08-01 00:00:00
abstract::When microbes lack the nutrients necessary for growth, they enter stationary phase. In cases when energy sources are still present in the environment, they must decide whether to continue to use their metabolic program to harvest the available energy. Here we characterized the metabolic response to a variety of types ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00061-14
更新日期:2014-05-01 00:00:00
abstract::Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.7.4363-4366.2004
更新日期:2004-07-01 00:00:00
abstract::The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1379-1384.1985
更新日期:1985-06-01 00:00:00
abstract::Legionella pneumophila is an intracellular parasite of protozoa and human phagocytes. To examine adaptation of this bacterium to parasitize protozoa, the sequence of events of the intracellular infection of the amoeba Hartmannella vermiformis was examined. The previously described uptake phenomenon of coiling phagocyt...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.6.2022-2028.1996
更新日期:1996-06-01 00:00:00
abstract::Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02555-20
更新日期:2021-01-29 00:00:00
abstract::A new membrane filter agar medium (MI agar) containing a chromogen, indoxyl-beta-D-glucuronide, and a fluorogen, 4-methylumbelliferyl-beta-D-galactopyranoside, was developed to simultaneously detect and enumerate Escherichia coli and total coliforms (TC) in water samples on the basis of their enzyme activities. TC pro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.11.3534-3544.1993
更新日期:1993-11-01 00:00:00
abstract::We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.1.111-119.1994
更新日期:1994-01-01 00:00:00
abstract::The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.11.3206-3211.1991
更新日期:1991-11-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Curr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01761-14
更新日期:2014-10-01 00:00:00
abstract::Previous attempts to express the toxin complex genes of Photorhabdus luminescens W14 in Escherichia coli have failed to reconstitute their oral toxicity to the model insect Manduca sexta. Here we show that the combination of three genes, tcdA, tcdB, and tccC, is essential for oral toxicity to M. sexta when expression ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.11.5017-5024.2001
更新日期:2001-11-01 00:00:00
abstract::Bacteria can survive on hospital textiles and surfaces, from which they can be disseminated, representing a source of health care-associated infections (HCAIs). Surfaces containing copper (Cu), which is known for its bactericidal properties, could be an efficient way to lower the burden of potential pathogens. The ant...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02266-12
更新日期:2012-12-01 00:00:00
abstract::Biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to CO2 and CO after 140 h of incubation by a pure culture of a type II methane-utili...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.3067-3071.1992
更新日期:1992-09-01 00:00:00
abstract::Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.3.1316-1319.1999
更新日期:1999-03-01 00:00:00
abstract::Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial acti...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07312-11
更新日期:2012-04-01 00:00:00
abstract::Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02897-09
更新日期:2010-04-01 00:00:00
abstract::This study aimed at investigating the antifungal activity of Wickerhamomyces anomalus and sourdough lactic acid bacteria to extend the shelf life of wheat flour bread. The antifungal activity was assayed by agar diffusion, growth rate inhibition, and conidial germination assays, using Penicillium roqueforti DPPMAF1 as...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02669-10
更新日期:2011-05-01 00:00:00
abstract::The persistence of hepatitis A virus (HAV) was determined both in mixtures of septic tank effluent (STE) with dairy cattle manure slurry (DCMS) and in mixtures of STE with swine manure slurry (SMS). HAV was consistently inactivated more rapidly in the two types of mixed wastes than in STE alone or in the control Dulbe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.1.87-91.1995
更新日期:1995-01-01 00:00:00
abstract::The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.7.2583-2588.1995
更新日期:1995-07-01 00:00:00
abstract::Bacillus thuringiensis produces large amounts of various pesticidal proteins during the stationary phase. In order to achieve a high yield and form crystals, some pesticidal proteins require the presence of other proteins. Helper protein P20 is required for efficient production of both the Cyt1A and Cry11A crystal pro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.12.5362-5369.2001
更新日期:2001-12-01 00:00:00
abstract::1,2-Dichloroethane (1,2-DCA) and 1,2-dibromoethane (ethylene dibromide [EDB]) contaminate groundwater at many hazardous waste sites. The objectives of this study were to measure yields, maximum specific growth rates (μ), and half-saturation coefficients (K(S)) in enrichment cultures that use 1,2-DCA and EDB as termina...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02163-12
更新日期:2013-02-01 00:00:00
abstract::A yellow-pigmented marine bacterium, designated strain SD-21, was isolated from surface sediments of San Diego Bay, San Diego, Calif., based on its ability to oxidize soluble Mn(II) to insoluble Mn(III, IV) oxides. 16S rRNA analysis revealed that this organism was most closely related to members of the genus Erythroba...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.4024-4029.2001
更新日期:2001-09-01 00:00:00
abstract::Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aur...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02641-08
更新日期:2009-07-01 00:00:00
abstract::The presence or absence of molecular oxygen has been shown to play a crucial role in the degradability of haloaromatic compounds. In the present study, it was shown that anaerobic phototrophic 3-chlorobenzoate (3CBA) metabolism by Rhodopseudomonas palustris DCP3 is oxygen tolerant up to a concentration of 3 microM O2....
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.1.131-137.1999
更新日期:1999-01-01 00:00:00
abstract::Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.12.5431-5435.1999
更新日期:1999-12-01 00:00:00
abstract::Pseudomonas aeruginosa and Vibrio cholerae showed a strong positive chemotactic response towards rum distillery wastewaters (mostos) and a high oxygen uptake rate in the presence of this complex substrate. Rum slops stimulated only motility in Aeromonas hydrophila and Escherichia coli. The A. hydrophila and E. coli is...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.6.1438-1441.1983
更新日期:1983-12-01 00:00:00