Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract.

Abstract:

:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by molecular detection techniques based on specific PCR amplification of the nisin (nisA) gene from DNA extracted from human feces. A method was developed for the efficient extraction of microbial DNA from human feces. The results show that a fraction of viable cells of L. lactis TC165.5 survived passage through the human gastrointestinal tract. Only cells that passed within 3 days of ingestion could be recovered from the feces of the volunteers, and they accounted for approximately 1% of the total number of cells consumed. The presence of nisA in DNA extracted from feces could be detected up to 4 days, when viable cells were no longer present.

journal_name

Appl Environ Microbiol

authors

Klijn N,Weerkamp AH,de Vos WM

doi

10.1128/AEM.61.7.2771-2774.1995

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

2771-4

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

61

pub_type

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