Abstract:
:Staphylococcus aureus is a gram-positive pathogen that causes a variety of diseases, including bovine mastitis, which has severe economic consequences. Standard antibiotic treatment results in selection of resistant strains, leading to a need for alternative treatments, such as bacteriophage therapy. Forty-nine S. aureus isolates were obtained from the milk of mastitic cows for use in screening of staphylococcal phages. Fifteen isolates which were positive for both coagulase and hemolysin were assayed by PCR for variation in the X region and the immunoglobulin G-binding region of the protein A gene (spa) and in the carboxy terminus of the coagulase gene (coa) and for the presence of enterotoxin C, G, H, and I genes. The host ranges of 52 phages isolated from sewage influent were determined by performing spot tests with the 15 S. aureus isolates, and two phages were subsequently chosen for further analysis. PhiSA039 had the widest host range, producing clear plaques on 13 of the 15 isolates (87%), while PhiSA012 produced clear plaques on 8 isolates (53%) and was the only phage that produced a clear plaque on a nonmastitic S. aureus strain. Transmission electron microscopy revealed that the phages were similar sizes and belonged to the Myoviridae family. Measurement of optical densities during coculture with S. aureus isolates confirmed the breadth of the PhiSA039 host range and showed that PhiSA012 had potent lytic capability. PhiSA012-resistant bacteria did not appear for three of seven isolates tested (43%) after 65 h of incubation. These two phages are proposed as candidates for phage therapy of bovine mastitis.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Synnott AJ,Kuang Y,Kurimoto M,Yamamichi K,Iwano H,Tanji Ydoi
10.1128/AEM.02641-08subject
Has Abstractpub_date
2009-07-01 00:00:00pages
4483-90issue
13eissn
0099-2240issn
1098-5336pii
AEM.02641-08journal_volume
75pub_type
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