Characterization of phiLC3, a Lactococcus lactis subsp. cremoris temperature bacteriophage with cohesive single-stranded DNA ends.

abstract：:A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatogra...

journal_title：Applied and environmental microbiology

authors： Gendloff EH,Pestka JJ,Swanson SP,Hart LP

更新日期：1984-05-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Lundgren BV,Allard B,Rosswall T

更新日期：1988-07-01 00:00:00

abstract：:The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

journal_title：Applied and environmental microbiology

authors： Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

更新日期：2012-10-01 00:00:00

abstract：:Wax-coated capsules containing selective ingredients (brilliant green and oxgall) were added at the time of inoculation of most-probable-number media (modified lactose broths). The inhibitory ingredients gradually diffused from the capsules into the nonselective media, imparting selectivity to the media. Concentration...

journal_title：Applied and environmental microbiology

authors： Lanz WW,Hartman PA

更新日期：1976-11-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:The utilization of the fuel oxygenate methyl tert-butyl ether (MTBE) and related compounds by microorganisms was investigated in a mainly theoretical study based on the Y(ATP) concept. Experiments were conducted to derive realistic maintenance coefficients and K(s) values needed to calculate substrate fluxes available...

journal_title：Applied and environmental microbiology

authors： Müller RH,Rohwerder T,Harms H

更新日期：2007-03-01 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00

abstract：:Escherichia coli O157:H7 causes hemorrhagic colitis and life-threatening complications. Because healthy cattle are reservoirs for the bacterium, ruminant infection models have applications in analyzing the relationship between cattle and this human pathogen and in testing interventions to reduce or prevent bovine colo...

journal_title：Applied and environmental microbiology

authors： Sheng H,Davis MA,Knecht HJ,Hovde CJ

更新日期：2004-08-01 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:Bioaugmentation can alter the potential activity as well as the composition of the naturally occurring microbial biota during bioremediation of a contaminated site. The focus of the current study is the pollutant 17β-estradiol (E2), which can cause endocrine effects and is potentially harmful to aquatic biota and to p...

journal_title：Applied and environmental microbiology

authors： Iasur-Kruh L,Hadar Y,Minz D

更新日期：2011-06-01 00:00:00

abstract：:We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...

journal_title：Applied and environmental microbiology

authors： Pankowski JA,Puckett SM,Nano FE

更新日期：2016-01-15 00:00:00

abstract：:A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

journal_title：Applied and environmental microbiology

authors： Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

更新日期：1976-09-01 00:00:00

abstract：:A xanthanase complex secreted by a consortium of heat-stable, salt-tolerant bacteria includes a lyase that specifically removes terminal pyruvated beta-d-mannose residues from the side chains of xanthan gum. The enzyme was purified to homogeneity from the culture broth following ion-exchange chromatography and gel per...

journal_title：Applied and environmental microbiology

authors： Ahlgren JA

更新日期：1991-09-01 00:00:00

abstract：:Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams...

journal_title：Applied and environmental microbiology

authors： Kakoyiannis CK,Winter PJ,Marshall RB

更新日期：1984-09-01 00:00:00

abstract：:Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

journal_title：Applied and environmental microbiology

authors： Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

更新日期：2003-06-01 00:00:00

abstract：:The chromate reductase purified from Pseudomonas ambigua was found to be homologous with several nitroreductases. Escherichia coli DH5alpha and Vibrio harveyi KCTC 2720 nitroreductases were chosen for the present study, and their chromate-reducing activities were determined. A fusion between glutathione S-transferase ...

journal_title：Applied and environmental microbiology

authors： Kwak YH,Lee DS,Kim HB

更新日期：2003-08-01 00:00:00

abstract：:A new membrane filter agar medium (MI agar) containing a chromogen, indoxyl-beta-D-glucuronide, and a fluorogen, 4-methylumbelliferyl-beta-D-galactopyranoside, was developed to simultaneously detect and enumerate Escherichia coli and total coliforms (TC) in water samples on the basis of their enzyme activities. TC pro...

journal_title：Applied and environmental microbiology

authors： Brenner KP,Rankin CC,Roybal YR,Stelma GN Jr,Scarpino PV,Dufour AP

更新日期：1993-11-01 00:00:00

abstract：:Members of the genus Sphingomonas are important catalysts for removal of polycyclic aromatic hydrocarbons (PAHs) in soil, but their activity can be affected by various stress factors. This study examines the physiological and genome-wide transcription response of the phenanthrene-degrading Sphingomonas sp. strain LH12...

journal_title：Applied and environmental microbiology

authors： Fida TT,Breugelmans P,Lavigne R,Coronado E,Johnson DR,van der Meer JR,Mayer AP,Heipieper HJ,Hofkens J,Springael D

更新日期：2012-12-01 00:00:00

abstract：:Bacillus thuringiensis vegetative insecticidal proteins (Vip3A) have been recently introduced in important crops as a strategy to delay the emerging resistance to the existing Cry toxins. The mode of action of Vip3A proteins has been studied in Spodoptera frugiperda with the aim of characterizing their binding to the ...

journal_title：Applied and environmental microbiology

authors： Chakroun M,Ferré J

更新日期：2014-10-01 00:00:00

abstract：:We recently showed that expressing an H(2)O-NADH oxidase in Saccharomyces cerevisiae drastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accu...

journal_title：Applied and environmental microbiology

authors： Heux S,Sablayrolles JM,Cachon R,Dequin S

更新日期：2006-09-01 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

journal_title：Applied and environmental microbiology

authors： Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

更新日期：2012-12-01 00:00:00

abstract：:The doses of UV irradiation necessary to inactivate selected enteric viruses on the U.S. Environmental Protection Agency Contaminant Candidate List were determined. Three-log reductions of echovirus 1, echovirus 11, coxsackievirus B3, coxsackievirus B5, poliovirus 1, and human adenovirus type 2 were effected by doses ...

journal_title：Applied and environmental microbiology

authors： Gerba CP,Gramos DM,Nwachuku N

更新日期：2002-10-01 00:00:00

abstract：:Filamentous fungi are widely used for the production of homologous and heterologous proteins. Recently, there has been increasing interest in Aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. To provide an overview of protein secretion by A. oryzae in solid-state cultur...

journal_title：Applied and environmental microbiology

authors： Oda K,Kakizono D,Yamada O,Iefuji H,Akita O,Iwashita K

更新日期：2006-05-01 00:00:00

abstract：:A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

journal_title：Applied and environmental microbiology

authors： Lee C,Langlois BE,Dawson KA

更新日期：1993-05-01 00:00:00

abstract：:Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

journal_title：Applied and environmental microbiology

authors： Fallon RD

更新日期：1992-09-01 00:00:00

abstract：:Diacetyl is a by-product of pyruvate metabolism in Lactococcus lactis, where pyruvate is first converted to alpha-acetolactate, which is slowly decarboxylated to diacetyl in the presence of oxygen. L. lactis usually converts alpha-acetolactate to acetoin enzymatically, by alpha-acetolactate decarboxylase encoded by th...

journal_title：Applied and environmental microbiology

authors： Goupil N,Corthier G,Ehrlich SD,Renault P

更新日期：1996-07-01 00:00:00

abstract：:To observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (Danio rerio), a series of plasmids was constructed for the red fluorescent protein (RFP) labeling of a variety of fish and human pathogens. The aim of this study was to create a collection o...

journal_title：Applied and environmental microbiology

authors： Singer JT,Phennicie RT,Sullivan MJ,Porter LA,Shaffer VJ,Kim CH

更新日期：2010-06-01 00:00:00

abstract：:Virulence gene profiles of atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) from cattle, sheep, and humans were examined to determine the relationship between pathotypes. Shared virulence factors (intimin, EHEC hemolysin, serine protease, and a type II secretion system) were ...

journal_title：Applied and environmental microbiology

authors： Cookson AL,Cao M,Bennett J,Nicol C,Thomson-Carter F,Attwood GT

更新日期：2010-06-01 00:00:00