Extensive manipulation of caseicins A and B highlights the tolerance of these antimicrobial peptides to change.

Abstract:

:Caseicins A and B are low-molecular-weight antimicrobial peptides which are released by proteolytic digestion of sodium caseinate. Caseicin A (IKHQGLPQE) is a nine-amino-acid cationic peptide, and caseicin B (VLNENLLR) is a neutral eight-amino-acid peptide; both have previously been shown to exhibit antibacterial activity against a number of pathogens, including Cronobacter sakazakii. Previously, four variants of each caseicin which differed subtly from their natural counterparts were generated by peptide synthesis. Antimicrobial activity assays revealed that the importance of a number of the residues within the peptides was dependent on the strain being targeted. In this study, this engineering-based approach was expanded through the creation of a larger collection of 26 peptides which are altered in a variety of ways. The investigation highlights the generally greater tolerance of caseicin B to change, the fact that changes have a more detrimental impact on anti-Gram-negative activity, and the surprising number of variants which exhibit enhanced activity against Staphylococcus aureus.

journal_name

Appl Environ Microbiol

authors

Norberg S,O'Connor PM,Stanton C,Ross RP,Hill C,Fitzgerald GF,Cotter PD

doi

10.1128/AEM.07312-11

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

2353-8

issue

7

eissn

0099-2240

issn

1098-5336

pii

AEM.07312-11

journal_volume

78

pub_type

杂志文章
  • Production of sterigmatocystin by Aspergillus versicolor and Bipolaris sorokiniana on semisynthetic liquid and solid media.

    abstract::Higher yields of sterigmatocystin were obtained with Aspergillus versicolor than with Bipolaris sorokiniana both in liquid and on solid media. The optimum temperature for sterigmatocystin production by A. versicolor was 27 to 29 degrees C and 23 degrees C for B. sorokiniana. In liquid shake cultures, production of ste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.2.206-208.1976

    authors: Rabie CJ,Lubben A,Steyn M

    更新日期:1976-08-01 00:00:00

  • Competitive exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a sequencing fed-batch culture.

    abstract::Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.555-559.2002

    authors: van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

    更新日期:2002-02-01 00:00:00

  • Biodegradation of ortho-cresol by a mixed culture of nitrate-reducing bacteria growing on toluene.

    abstract::A mixed culture of nitrate-reducing bacteria degraded o-cresol in the presence of toulene as a primary growth substrate. No degradation of o-cresol was observed in the absence of toluene or when the culture grew on p-cresol and 2,4-dimethylphenol. In batch cultures, the degradation of o-cresol started after toluene wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.7.2286-2292.1993

    authors: Flyvbjerg J,Jørgensen C,Arvin E,Jensen BK,Olsen SK

    更新日期:1993-07-01 00:00:00

  • Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.

    abstract::Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02238-07

    authors: Gursinsky T,Gröbe D,Schierhorn A,Jäger J,Andreesen JR,Söhling B

    更新日期:2008-03-01 00:00:00

  • Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

    abstract::A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4432-4439.2004

    authors: Chen H,Ponniah G,Salonen N,Blum P

    更新日期:2004-08-01 00:00:00

  • Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization.

    abstract::The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.1.397-400.2002

    authors: Solomon EB,Yaron S,Matthews KR

    更新日期:2002-01-01 00:00:00

  • Glutathione reductase-mediated synthesis of tellurium-containing nanostructures exhibiting antibacterial properties.

    abstract::Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02207-14

    authors: Pugin B,Cornejo FA,Muñoz-Díaz P,Muñoz-Villagrán CM,Vargas-Pérez JI,Arenas FA,Vásquez CC

    更新日期:2014-11-01 00:00:00

  • Verocytotoxin-producing Escherichia coli in wild birds and rodents in close proximity to farms.

    abstract::Wild animals living close to cattle and pig farms (four each) were examined for verocytotoxin-producing Escherichia coli (VTEC; also known as Shiga toxin-producing E. coli). The prevalence of VTEC among the 260 samples from wild animals was generally low. However, VTEC isolates from a starling (Sturnus vulgaris) and a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.11.6944-6947.2004

    authors: Nielsen EM,Skov MN,Madsen JJ,Lodal J,Jespersen JB,Baggesen DL

    更新日期:2004-11-01 00:00:00

  • Listeria phage and phage tail induction triggered by components of bacterial growth media (phosphate, LiCl, nalidixic acid, and acriflavine).

    abstract::The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03235-14

    authors: Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

    更新日期:2015-03-01 00:00:00

  • Role of absolute humidity in the inactivation of influenza viruses on stainless steel surfaces at elevated temperatures.

    abstract::Influenza virus has been found to persist in the environment for hours to days, allowing for secondary transmission of influenza via inanimate objects known as fomites. We evaluated the efficacy of heat and moisture for the decontamination of surfaces for the purpose of preventing of the spread of influenza. Aqueous s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02674-09

    authors: McDevitt J,Rudnick S,First M,Spengler J

    更新日期:2010-06-01 00:00:00

  • Detection and counting of Nitrobacter populations in soil by PCR.

    abstract::Although the biological conversion of nitrite to nitrate is a well-known process, studies of Nitrobacter populations are hindered by their physiological characteristics. This report describes a new method for detecting and counting Nitrobacter populations in situ with the PCR. Two primers from the 16S rRNA gene were u...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.6.2093-2098.1995

    authors: Degrange V,Bardin R

    更新日期:1995-06-01 00:00:00

  • A Counterselectable Sucrose Sensitivity Marker Permits Efficient and Flexible Mutagenesis in Streptococcus agalactiae.

    abstract::Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03009-18

    authors: Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

    更新日期:2019-03-22 00:00:00

  • Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens.

    abstract::Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol)...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.4.890-897.1988

    authors: Rasmussen MA,Hespell RB,White BA,Bothast RJ

    更新日期:1988-04-01 00:00:00

  • Dehalogenation in marine sediments containing natural sources of halophenols.

    abstract::Halophenols such as 2,4-dibromophenol (DBP) occur naturally in some marine sediments, as a consequence of various animal and algal activities. In an earlier study, DBP was observed in the burrow microenvironment of the hemichordate Saccoglossus kowalewskii. At the concentrations found in the burrow lining, aerobic res...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.3079-3085.1988

    authors: King GM

    更新日期:1988-12-01 00:00:00

  • Transformation of Dibenzo-p-Dioxin by Pseudomonas sp. Strain HH69.

    abstract::Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.1157-1159.1990

    authors: Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

    更新日期:1990-04-01 00:00:00

  • Factors limiting the spread of the protective symbiont Hamiltonella defensa in Aphis craccivora Aphids.

    abstract::Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01775-14

    authors: Dykstra HR,Weldon SR,Martinez AJ,White JA,Hopper KR,Heimpel GE,Asplen MK,Oliver KM

    更新日期:2014-09-01 00:00:00

  • Minimum threshold for hydrogen metabolism in methanogenic bacteria.

    abstract::Methanogenic isolates did not consume hydrogen below partial pressures of 6.5 Pa. Thus, in contrast to a previous report, results from pure-culture studies do not invalidate the threshold model for methane production from hydrogen in sediments. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.6.1530-1531.1985

    authors: Lovley DR

    更新日期:1985-06-01 00:00:00

  • Biochemical characterization of ochratoxin A-producing strains of the genus Penicillium.

    abstract::In order to explore the biochemical scope of ochratoxin A-producing penicillia, we screened 48 Penicillium verrucosum isolates for the production of secondary metabolites. Fungal metabolites were analyzed by high-pressure liquid or gas chromatography coupled to diode array detection or mass spectrometry. The following...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3630-3635.2001

    authors: Larsen TO,Svendsen A,Smedsgaard J

    更新日期:2001-08-01 00:00:00

  • Maintenance of Laboratory strains of obligately anaerobic rumen bacteria.

    abstract::Cultures of rumen bacteria can be stored at -20 degrees C for at least 2 years in a liquid medium containing 20% glycerol. Thawing, sampling, and refreezing do not significantly affect viability. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.499-501.1982

    authors: Teather RM

    更新日期:1982-08-01 00:00:00

  • Relationship of the Presence and Copy Number of Plasmids to Exopolysaccharide Production and Symbiotic Effectiveness in Rhizobium fredii USDA 206.

    abstract::Rhizobium fredii USDA 206 harbors four large plasmids, one of which carries nodulation and nitrogen fixation genes. Previously isolated groups of plasmid-cured derivatives of strain USDA 206 were compared with each other to determine possible plasmid functions. Mutant strain 206CANS was isolated as a nonmucoid (Muc) d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.4.813-818.1989

    authors: Barbour WM,Elkan GH

    更新日期:1989-04-01 00:00:00

  • Effect of different holding regimens on the intestinal microflora of herring (Clupea harengus) larvae.

    abstract::The aerobic intestinal microflora of 2-week-old herring (Clupea harengus) larvae was characterized by using conventional microbiological methods and electron microscopy. Larvae were hatched and kept in filtered seawater or in seawater with penicillin and streptomycin. The gastrointestinal tract of herring larvae is es...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.2.461-470.1992

    authors: Hansen GH,Strøm E,Olafsen JA

    更新日期:1992-02-01 00:00:00

  • Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients.

    abstract::Located in southwest China, the Aha watershed is continually contaminated by acid mine drainage (AMD) produced from upstream abandoned coal mines. The watershed is fed by creeks with elevated concentrations of aqueous Fe (total Fe > 1 g/liter) and SO4 (2-) (>6 g/liter). AMD contamination gradually decreases throughout...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00935-15

    authors: Sun W,Xiao T,Sun M,Dong Y,Ning Z,Xiao E,Tang S,Li J

    更新日期:2015-08-01 00:00:00

  • Oxygen requirements of the food spoilage yeast Zygosaccharomyces bailii in synthetic and complex media.

    abstract::Most yeast species can ferment sugars to ethanol, but only a few can grow in the complete absence of oxygen. Oxygen availability might, therefore, be a key parameter in spoilage of food caused by fermentative yeasts. In this study, the oxygen requirement and regulation of alcoholic fermentation were studied in batch c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.5.2123-2128.2001

    authors: Rodrigues F,Côrte-Real M,Leão C,van Dijken JP,Pronk JT

    更新日期:2001-05-01 00:00:00

  • Evidence of hydrolytic route for anaerobic cyanide degradation.

    abstract::Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.3163-3164.1992

    authors: Fallon RD

    更新日期:1992-09-01 00:00:00

  • Cloning of the Clostridium acetobutylicum ATCC 824 acetyl coenzyme A acetyltransferase (thiolase; EC 2.3.1.9) gene.

    abstract::Thiolase (acetyl coenzyme A acetyltransferase; EC 2.3.1.9) from Clostridium acetobutylicum is a key enzyme in the production of acids and solvents in this organism. The purification and properties of the enzyme have already been described (D. P. Wiesenborn, F. B. Rudolph, and E.T. Papoutsakis, Appl. Environ. Microbiol...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2735-2741.1991

    authors: Petersen DJ,Bennett GN

    更新日期:1991-09-01 00:00:00

  • Comparative inactivation of enteroviruses and adenovirus 2 by UV light.

    abstract::The doses of UV irradiation necessary to inactivate selected enteric viruses on the U.S. Environmental Protection Agency Contaminant Candidate List were determined. Three-log reductions of echovirus 1, echovirus 11, coxsackievirus B3, coxsackievirus B5, poliovirus 1, and human adenovirus type 2 were effected by doses ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.5167-5169.2002

    authors: Gerba CP,Gramos DM,Nwachuku N

    更新日期:2002-10-01 00:00:00

  • Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples.

    abstract::PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02878-08

    authors: Delgado-Viscogliosi P,Solignac L,Delattre JM

    更新日期:2009-06-01 00:00:00

  • Novel antibiotic-free plasmid selection system based on complementation of host auxotrophy in the NAD de novo synthesis pathway.

    abstract::The use of antibiotic resistance genes in plasmids causes potential biosafety and clinical hazards, such as the possibility of horizontal spread of resistance genes or the rapid emergence of multidrug-resistant pathogens. This paper introduces a novel auxotrophy complementation system that allowed plasmids and host ce...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02462-09

    authors: Dong WR,Xiang LX,Shao JZ

    更新日期:2010-04-01 00:00:00

  • Trifolitoxin Production Increases Nodulation Competitiveness of Rhizobium etli CE3 under Agricultural Conditions.

    abstract::A major barrier to the use of nitrogen-fixing inoculum strains for the enhancement of legume productivity is the inability of commercially available strains to compete with indigenous rhizobia for nodule formation. Despite extensive research on nodulation competitiveness, there are no examples of field efficacy studie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2630-2633.1998

    authors: Robleto EA,Kmiecik K,Oplinger ES,Nienhuis J,Triplett EW

    更新日期:1998-07-01 00:00:00

  • Highly efficient gluten degradation by lactobacilli and fungal proteases during food processing: new perspectives for celiac disease.

    abstract::Presently, the only effective treatment for celiac disease is a life-long gluten-free diet. In this work, we used a new mixture of selected sourdough lactobacilli and fungal proteases to eliminate the toxicity of wheat flour during long-time fermentation. Immunological (R5 antibody-based sandwich and competitive enzym...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00260-07

    authors: Rizzello CG,De Angelis M,Di Cagno R,Camarca A,Silano M,Losito I,De Vincenzi M,De Bari MD,Palmisano F,Maurano F,Gianfrani C,Gobbetti M

    更新日期:2007-07-01 00:00:00