Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens.

Abstract:

:Highly methylated, long-chain celluloses strongly inhibited cellulose degradation by several species of cellulolytic bacteria of ruminal origin. Specifically, the inhibitory effects of methylcellulose on the growth of Ruminococcus flavefaciens FD1 were concentration dependent, with complete inhibition at 0.1% (wt/vol). However, methylcellulose did not inhibit growth on cellobiose or cellulooligosaccharides. Mixtures of methylated cellulooligosaccharides having an average degree of polymerization of 6.7 to 9.5 inhibited cellulose degradation, but those with an average degree of polymerization of 1.0 to 4.5 did not. Similar inhibitory effects by methylcellulose and, to a lesser extent, by methyl cellulooligosaccharides were observed on cellulase activity, as measured by hydrolysis of p-nitrophenyl-beta-d-cellobioside. R. flavefaciens cultures hydrolyzed cellulooligosaccharides to cellobiose and cellotriose as final end products. Cellopentaose and cellohexaose were cleaved to these end products, but cellotetraose was also formed from cellohexaose. Methylcellulose did not inhibit hydrolysis of cellulooligosaccharides. These data are consistent with the presence of separate cellulase (beta-1,4-glucanase) and cellulodextrinase activities in R. flavefaciens.

journal_name

Appl Environ Microbiol

authors

Rasmussen MA,Hespell RB,White BA,Bothast RJ

doi

10.1128/AEM.54.4.890-897.1988

keywords:

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

890-7

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

54

pub_type

杂志文章
  • Relationship between Phylogeny and Pathotype for the Bacterial Blight Pathogen of Rice.

    abstract::Several transposable elements were isolated from the genome of Xanthomonas oryzae pv. oryzae. These elements and an avirulence gene isolated from X. oryzae pv. oryzae were used as hybridization probes for a collection of X. oryzae pv. oryzae strains from the Philippines. Each of the sequences was present in multiple c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.9.3275-3283.1994

    authors: Nelson RJ,Baraoidan MR,Cruz CM,Yap IV,Leach JE,Mew TW,Leung H

    更新日期:1994-09-01 00:00:00

  • Demethylation of dimethylsulfoniopropionate to 3-mercaptopropionate by an aerobic marine bacterium.

    abstract::A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.12.4617-4619.1994

    authors: Visscher PT,Taylor BF

    更新日期:1994-12-01 00:00:00

  • Fusobacterium nucleatum ATCC 10953 requires Actinomyces naeslundii ATCC 43146 for growth on saliva in a three-species community that includes Streptococcus oralis 34.

    abstract::Formation of dental plaque is a developmental process involving initial and late colonizing species that form polymicrobial communities. Fusobacteria are the most numerous gram-negative bacteria in dental plaque, but they become prevalent after the initial commensal colonizers, such as streptococci and actinomyces, ha...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02901-08

    authors: Periasamy S,Chalmers NI,Du-Thumm L,Kolenbrander PE

    更新日期:2009-05-01 00:00:00

  • Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.

    abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00239-15

    authors: Maisuria VB,Hosseinidoust Z,Tufenkji N

    更新日期:2015-06-01 00:00:00

  • Direct effects of UV-B radiation on the freshwater heterotrophic nanoflagellate Paraphysomonas sp.

    abstract::The formation of DNA photoproducts in organisms exposed to ambient levels of UV-B radiation can lead to death and/or reduced population growth in aquatic systems. Dependence on photoenzymatic repair to reverse DNA damage caused by UV-B radiation is demonstrated for Paraphysomonas sp., a member of a widely distributed ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02803-08

    authors: Macaluso AL,Mitchell DL,Sanders RW

    更新日期:2009-07-01 00:00:00

  • Soil Aggregate Microbial Communities: Towards Understanding Microbiome Interactions at Biologically Relevant Scales.

    abstract::Soils contain a tangle of minerals, water, nutrients, gases, plant roots, decaying organic matter, and microorganisms which work together to cycle nutrients and support terrestrial plant growth. Most soil microorganisms live in periodically interconnected communities closely associated with soil aggregates, i.e., smal...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.00324-19

    authors: Wilpiszeski RL,Aufrecht JA,Retterer ST,Sullivan MB,Graham DE,Pierce EM,Zablocki OD,Palumbo AV,Elias DA

    更新日期:2019-07-01 00:00:00

  • Intersporal Genetic Variation of Gigaspora margarita, a Vesicular Arbuscular Mycorrhizal Fungus, Revealed by M13 Minisatellite-Primed PCR.

    abstract::Spores of vesicular arbuscular mycorrhizal (VAM) fungi contain thousands of nuclei. In order to understand the karyotic structure of a VAM fungus spore, the genetic variation of the first generation of spores from a VAM fungus (Gigaspora margarita) was examined. Spores originating from both single- and multispore inoc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.2.676-678.1997

    authors: Zeze A,Sulistyowati E,Ophel-Keller K,Barker S,Smith S

    更新日期:1997-02-01 00:00:00

  • Effects of Nutrient Deprivation on Vibrio cholerae.

    abstract::[This corrects the article on p. 931 in vol. 46.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.5.1193-1193.1984

    authors:

    更新日期:1984-05-01 00:00:00

  • Clinically Unreported Salmonellosis Outbreak Detected via Comparative Genomic Analysis of Municipal Wastewater Salmonella Isolates.

    abstract::Municipal wastewater includes human waste that contains both commensal and pathogenic enteric microorganisms, and this collective community microbiome can be monitored for community diseases. In a previous study, we assessed the salmonellosis disease burden using municipal wastewater from Honolulu, Hawaii, which was m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00139-19

    authors: Diemert S,Yan T

    更新日期:2019-05-02 00:00:00

  • Investigation of genotypes of Borrelia burgdorferi in Ixodes scapularis ticks collected during surveillance in Canada.

    abstract::The genetic diversity of Borrelia burgdorferi sensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigate B. burgdorferi diversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adult Ixod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02636-10

    authors: Ogden NH,Margos G,Aanensen DM,Drebot MA,Feil EJ,Hanincová K,Schwartz I,Tyler S,Lindsay LR

    更新日期:2011-05-01 00:00:00

  • Transformation of sulfur compounds by an abundant lineage of marine bacteria in the alpha-subclass of the class Proteobacteria.

    abstract::Members of a group of marine bacteria that is numerically important in coastal seawater and sediments were characterized with respect to their ability to transform organic and inorganic sulfur compounds. Fifteen strains representing the Roseobacter group (a phylogenetic cluster of marine bacteria in the alpha-subclass...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.3810-3819.1999

    authors: González JM,Kiene RP,Moran MA

    更新日期:1999-09-01 00:00:00

  • Gene transfer by transduction in the marine environment.

    abstract::To determine the potential for bacteriophage-mediated gene transfer in the marine environment, we established transduction systems by using marine phage host isolates. Plasmid pQSR50, which contains transposon Tn5 and encodes kanamycin and streptomycin resistance, was used in plasmid transduction assays. Both marine b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.2780-2787.1998

    authors: Jiang SC,Paul JH

    更新日期:1998-08-01 00:00:00

  • Numerical dominance of a group of marine bacteria in the alpha-subclass of the class Proteobacteria in coastal seawater.

    abstract::A cluster of marine bacteria within the alpha-3 subclass of the class Proteobacteria accounted for up to 28% of the 16S ribosomal DNA (rDNA) sequences in seawater samples from the coast of the southeastern United States. Two independent oligonucleotide probes targeting 16S rDNA of this "marine alpha" cluster indicate ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.11.4237-4242.1997

    authors: González JM,Moran MA

    更新日期:1997-11-01 00:00:00

  • High-pressure inactivation of human norovirus virus-like particles provides evidence that the capsid of human norovirus is highly pressure resistant.

    abstract::Human norovirus (NoV) is the leading cause of nonbacterial acute gastroenteritis epidemics worldwide. High-pressure processing (HPP) has been considered a promising nonthermal processing technology to inactivate food- and waterborne viral pathogens. Due to the lack of an effective cell culture method for human NoV, th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00532-12

    authors: Lou F,Huang P,Neetoo H,Gurtler JB,Niemira BA,Chen H,Jiang X,Li J

    更新日期:2012-08-01 00:00:00

  • Characterization of Pseudomonas maltophilia Isolates from Fleece Rot.

    abstract::A total of 286 isolates of Pseudomonas maltophilia was collected from sheep exhibiting brown or yellowish fleece rot and from fly-strike lesions. Enzyme activities for 10 of the isolates were examined by plate tests and with the API ZYM system and compared with the enzymatic profile of a human type strain of P. maltop...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.2.346-348.1986

    authors: Macdiarmid JA,Burrell DH

    更新日期:1986-02-01 00:00:00

  • Multiple Environmental Factors Influence the Importance of the Phosphodiesterase DipA upon Pseudomonas aeruginosa Swarming.

    abstract::Pseudomonas aeruginosa exhibits flagellum-mediated swimming in liquid and swarming on hydrated surfaces under diverse nutrient conditions. Prior studies have implicated a phosphodiesterase, DipA, in regulating these flagellum-mediated motilities, but collectively, the necessity for DipA was unclear. In this study, we ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02847-17

    authors: Mattingly AE,Kamatkar NG,Morales-Soto N,Borlee BR,Shrout JD

    更新日期:2018-03-19 00:00:00

  • Sulfate-reducing bacteria in tubes constructed by the marine infaunal polychaete Diopatra cuprea.

    abstract::Marine infaunal burrows and tubes greatly enhance solute transport between sediments and the overlying water column and are sites of elevated microbial activity. Biotic and abiotic controls of the compositions and activities of burrow and tube microbial communities are poorly understood. The microbial communities in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7053-7065.2004

    authors: Matsui GY,Ringelberg DB,Lovell CR

    更新日期:2004-12-01 00:00:00

  • Analysis of exopolysaccharide production by Lactobacillus casei CG11, isolated from cheese.

    abstract::Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.12.4086-4088.1992

    authors: Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

    更新日期:1992-12-01 00:00:00

  • Chromium reduction in Pseudomonas putida.

    abstract::Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat lab...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.7.2268-2270.1990

    authors: Ishibashi Y,Cervantes C,Silver S

    更新日期:1990-07-01 00:00:00

  • Current production and metal oxide reduction by Shewanella oneidensis MR-1 wild type and mutants.

    abstract::Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01087-07

    authors: Bretschger O,Obraztsova A,Sturm CA,Chang IS,Gorby YA,Reed SB,Culley DE,Reardon CL,Barua S,Romine MF,Zhou J,Beliaev AS,Bouhenni R,Saffarini D,Mansfeld F,Kim BH,Fredrickson JK,Nealson KH

    更新日期:2007-11-01 00:00:00

  • Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

    abstract::Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.901-908.2003

    authors: Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

    更新日期:2003-02-01 00:00:00

  • Rapid multiplex detection and differentiation of Listeria cells by use of fluorescent phage endolysin cell wall binding domains.

    abstract::The genus Listeria comprises food-borne pathogens associated with severe infections and a high mortality rate. Endolysins from bacteriophages infecting Listeria are promising tools for both their detection and control. These proteins feature a modular organization, consisting of an N-terminal enzymatically active doma...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00801-10

    authors: Schmelcher M,Shabarova T,Eugster MR,Eichenseher F,Tchang VS,Banz M,Loessner MJ

    更新日期:2010-09-01 00:00:00

  • Sporicidal activity of synthetic antifungal undecapeptides and control of Penicillium rot of apples.

    abstract::The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00711-09

    authors: Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

    更新日期:2009-09-01 00:00:00

  • Gene expression induced in Escherichia coli O157:H7 upon exposure to model apple juice.

    abstract::Escherichia coli O157:H7 has caused serious outbreaks of food-borne illness via transmission in a variety of food vehicles, including unpasteurized apple juice, dried salami, and spinach. To understand how this pathogen responds to the multiple stresses of the food environment, we compared global transcription pattern...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02841-08

    authors: Bergholz TM,Vanaja SK,Whittam TS

    更新日期:2009-06-01 00:00:00

  • Increase in acid tolerance of Campylobacter jejuni through coincubation with amoebae.

    abstract::Campylobacter jejuni is a recognized and common gastrointestinal pathogen in most parts of the world. Human infections are often food borne, and the bacterium is frequent among poultry and other food animals. However, much less is known about the epidemiology of C. jejuni in the environment and what mechanisms the bac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01219-09

    authors: Axelsson-Olsson D,Svensson L,Olofsson J,Salomon P,Waldenström J,Ellström P,Olsen B

    更新日期:2010-07-01 00:00:00

  • Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay.

    abstract::A monoclonal antibody (BA11) has been produced against Clostridium botulinum type A neurotoxin by the fusion of myeloma cells (P3 NS1/1-Ag4-1) with spleen cells from BALB/c mice immunized with botulinum type A neurotoxoid. The antibody bound specifically to botulinum type A neurotoxin, showing no cross-reactivity with...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.1.63-67.1985

    authors: Shone C,Wilton-Smith P,Appleton N,Hambleton P,Modi N,Gatley S,Melling J

    更新日期:1985-07-01 00:00:00

  • Surface Sensing for Paenibacillus sp. NAIST15-1 Flagellar Gene Expression on Solid Medium.

    abstract::A rhizosphere Gram-positive bacterial isolate, Paenibacillus sp. NAIST15-1, exhibits intriguing motility behavior on hard agar medium. Paenibacillus sp. shows increased transcription of flagellar genes and hyperflagellation when transferred from liquid to solid medium. Hyperflagellated cells form wandering colonies th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00585-17

    authors: Kobayashi K,Kanesaki Y,Yoshikawa H

    更新日期:2017-07-17 00:00:00

  • Prevalence of enterotoxigenic Escherichia coli in some processed raw food from animal origin.

    abstract::Eighteen of 1,200 colonies of Escherichia coli isolated from "keebe," hamburger, or sausage produced heat-labile enterotoxin. None of them produced heat-stable enterotoxin. The characteristics of 9 of the 18 strains are presented. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.270-271.1980

    authors: Reis MH,Vasconcelos JC,Trabulsi LR

    更新日期:1980-01-01 00:00:00

  • Physiological characterization of strain DCB-1, a unique dehalogenating sulfidogenic bacterium.

    abstract::Strain DCB-1 is an obligately anaerobic bacterium which carries out the reductive dehalogenation of halobenzoates and was previously known to grow only on pyruvate plus 20% ruminal fluid. When various electron acceptors were supplied, thiosulfate and sulfite were found to stimulate growth. Sulfide was produced from th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.2938-2943.1988

    authors: Stevens TO,Linkfield TG,Tiedje JM

    更新日期:1988-12-01 00:00:00

  • Existence of a hydrogen sulfide:ferric ion oxidoreductase in iron-oxidizing bacteria.

    abstract::The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.1.431-433.1992

    authors: Sugio T,White KJ,Shute E,Choate D,Blake RC

    更新日期:1992-01-01 00:00:00