Biodegradation of ortho-cresol by a mixed culture of nitrate-reducing bacteria growing on toluene.

Abstract:

:A mixed culture of nitrate-reducing bacteria degraded o-cresol in the presence of toulene as a primary growth substrate. No degradation of o-cresol was observed in the absence of toluene or when the culture grew on p-cresol and 2,4-dimethylphenol. In batch cultures, the degradation of o-cresol started after toluene was degraded to below 0.5 to 1.0 mg/liter but continued only for about 3 to 5 days after the depletion of toluene since the culture had a limited capacity for o-cresol degradation once toluene was depleted. The total amount of o-cresol degraded was proportional to the amount of toluene metabolized, with an average yield of 0.47 mg of o-cresol degraded per mg of toluene metabolized. Experiments with [ring-U-14C]o-cresol indicated that about 73% of the carbon from degraded o-cresol was mineralized to CO2 and about 23% was assimilated into biomass after the transient accumulation of unidentified water-soluble intermediates. A mathematical model based on a simplified Monod equation is used to describe the kinetics of o-cresol degradation. In this model, the biomass activity toward o-cresol is assumed to decay according to first-order kinetics once toluene is depleted. On the basis of nonlinear regression of the data, the maximum specific rate of o-cresol degradation was estimated to be 0.4 mg of o-cresol per mg of biomass protein per h, and the first-order decay constant for o-cresol-degrading biomass activity was estimated to be 0.15 h-1.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Appl Environ Microbiol

authors

Flyvbjerg J,Jørgensen C,Arvin E,Jensen BK,Olsen SK

doi

10.1128/AEM.59.7.2286-2292.1993

subject

Has Abstract

pub_date

1993-07-01 00:00:00

pages

2286-92

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Rapid evaluation of the mycobactericidal efficacy of disinfectants in the quantitative carrier test EN 14563 by using fluorescent Mycobacterium terrae.

    abstract::To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01660-09

    authors: Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

    更新日期:2010-01-01 00:00:00

  • Dramatic activation of antibiotic production in Streptomyces coelicolor by cumulative drug resistance mutations.

    abstract::We recently described a new method to activate antibiotic production in bacteria by introducing a mutation conferring resistance to a drug such as streptomycin, rifampin, paromomycin, or gentamicin. This method, however, enhanced antibiotic production by only up to an order of magnitude. Working with Streptomyces coel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02800-07

    authors: Wang G,Hosaka T,Ochi K

    更新日期:2008-05-01 00:00:00

  • Protozoan-induced regulation of cyclic lipopeptide biosynthesis is an effective predation defense mechanism for Pseudomonas fluorescens.

    abstract::Environmental bacteria are exposed to a myriad of biotic interactions that influence their function and survival. The grazing activity of protozoan predators significantly impacts the dynamics, diversification, and evolution of bacterial communities in soil ecosystems. To evade protozoan predation, bacteria employ var...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01272-09

    authors: Mazzola M,de Bruijn I,Cohen MF,Raaijmakers JM

    更新日期:2009-11-01 00:00:00

  • Disruption of the Oxidative Pentose Phosphate Pathway Stimulates High-Yield Production Using Resting Corynebacterium glutamicum in the Absence of External Electron Acceptors.

    abstract::Identifying and overcoming the limitations preventing efficient high-yield production of chemicals remain important tasks in metabolic engineering. In an attempt to rewire Corynebacterium glutamicum to produce ethanol, we attained a low yield (63% of the theoretical) when using resting cells on glucose, and large amou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02114-20

    authors: Shen J,Chen J,Solem C,Jensen PR,Liu JM

    更新日期:2020-11-24 00:00:00

  • Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.

    abstract::We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.11.4192-4194.1994

    authors: Charles TC,Doty SL,Nester EW

    更新日期:1994-11-01 00:00:00

  • Critical evaluation of membrane supports for use in quantitative hybridizations.

    abstract::The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.1.300-303.1996

    authors: Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

    更新日期:1996-01-01 00:00:00

  • Purification and characterization of two highly thermophilic alkaline lipases from Thermosyntropha lipolytica.

    abstract::Two thermostable lipases were isolated and characterized from Thermosyntropha lipolytica DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium which grows syntrophically with methanogens on lipids such as olive oil, utilizing only the liberated fatty acid moieties but not the glycerol. Lipases LipA and LipB...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01509-07

    authors: Salameh MA,Wiegel J

    更新日期:2007-12-01 00:00:00

  • Utilization of exogenous siderophores and natural catechols by Listeria monocytogenes.

    abstract::Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.4.1643-1645.1995

    authors: Simon N,Coulanges V,Andre P,Vidon DJ

    更新日期:1995-04-01 00:00:00

  • Inhibition of cereulide toxin synthesis by emetic Bacillus cereus via long-chain polyphosphates.

    abstract::Severe intoxications caused by the Bacillus cereus emetic toxin cereulide can hardly be prevented due to the ubiquitous distribution and heat resistance of spores and the extreme thermal and chemical stability of cereulide. It would therefore be desirable to inhibit cereulide synthesis during food manufacturing proces...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02259-10

    authors: Frenzel E,Letzel T,Scherer S,Ehling-Schulz M

    更新日期:2011-02-01 00:00:00

  • Soil Fungicides for Controlling Chytridiaceous Mycoparasites of Gigaspora margarita and Glomus fasciculatum.

    abstract::The percentage of azygospores of Gigaspora margarita with zoosporangia of chytridiaceous fungi (CF) was reduced significantly after agitating them in fenaminosulf before incubation in soil. Fenaminosulf did not affect zoosporangia development on chlamydospores of Glomus fasciculatum. Metalaxyl and ethazol were not eff...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.4.1306-1309.1983

    authors: Sylvia DM,Schenck NC

    更新日期:1983-04-01 00:00:00

  • Relationships among Cell Size, Membrane Permeability, and Preservative Resistance in Yeast Species.

    abstract::The rate of uptake of propanoic acid and the cell dimensions were measured for 23 yeasts differing in their resistance to weak-acid-type preservatives. Relationships between reciprocal uptake rate, reciprocal permeability, cell volume, cell area, volume/area, and the MICs of benzoic acid and propanoic acid for the yea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.11.2995-2999.1989

    authors: Warth AD

    更新日期:1989-11-01 00:00:00

  • Plant Cell Wall Carbohydrates as Substrates for Azospirillum brasiliense.

    abstract::Carbohydrate components (simple sugars and polysaccharides) of cell walls of pearl millet (Pennisetum americanum L., cv. Gahi) were studied as potential substrates for the root-associated diazotroph Azospirillum brasiliense Sp. 7. Simple sugars were utilized, but no evidence was obtained to support the suggestion that...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.12.2745-2748.1987

    authors: Myers ML,Hubbell DH

    更新日期:1987-12-01 00:00:00

  • Diversity of Rickettsiales in the microbiome of the lone star tick, Amblyomma americanum.

    abstract::Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02987-13

    authors: Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

    更新日期:2014-01-01 00:00:00

  • Failure of Ixodes ticks to inherit Borrelia afzelii infection.

    abstract::To define conditions promoting inherited infection by Lyme disease spirochetes in Ixodes ticks, we variously infected ticks with Borrelia afzelii and examined their progenies by dark-field microscopy, immunofluorescence, PCR, and serial passage. No episode of inherited infection was evident, regardless of instar or ge...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.3089-3091.1998

    authors: Matuschka FR,Schinkel TW,Klug B,Spielman A,Richter D

    更新日期:1998-08-01 00:00:00

  • Transformation of isopropylamine to L-alaninol by Pseudomonas sp. strain KIE171 involves N-glutamylated intermediates.

    abstract::Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or L-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with D-alaninol. To investigate the hypothesis that L-alaninol is an intermediate in the degradation of isopropylamine, two mini-Tn5 mutants unable to utilize both isopropylami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.5.2368-2375.2002

    authors: de Azevedo Wäsch SI,van der Ploeg JR,Maire T,Lebreton A,Kiener A,Leisinger T

    更新日期:2002-05-01 00:00:00

  • Effects of temperature abuse on survival of Vibrio vulnificus in oysters.

    abstract::Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.2771-2775.1992

    authors: Murphy SK,Oliver JD

    更新日期:1992-09-01 00:00:00

  • Distribution and diversity of archaea corresponding to the limnological cycle of a hypersaline stratified lake (Solar lake, Sinai, Egypt).

    abstract::The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.8.3269-3276.2000

    authors: Cytryn E,Minz D,Oremland RS,Cohen Y

    更新日期:2000-08-01 00:00:00

  • Origin of contamination and genetic diversity of Escherichia coli in beef cattle.

    abstract::The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.2794-2799.2003

    authors: Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

    更新日期:2003-05-01 00:00:00

  • Only one of the five Ralstonia solanacearum long-chain 3-ketoacyl-acyl carrier protein synthase homologues functions in fatty acid synthesis.

    abstract::Ralstonia solanacearum, a major phytopathogenic bacterium, causes a bacterial wilt disease in diverse plants. Although fatty acid analyses of total membranes of R. solanacearum showed that they contain primarily palmitic (C(16:0)), palmitoleic (C(16:1)) and cis-vaccenic (C(18:1)) acids, little is known regarding R. so...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07335-11

    authors: Cheng J,Ma J,Lin J,Fan ZC,Cronan JE,Wang H

    更新日期:2012-03-01 00:00:00

  • Effects of long-term treatment with acetylene on nitrogen-fixing microorganisms.

    abstract::Long periods of experimental incubation with acetylene led to a multifold enhancement of acetylene-reducing activity in Anabaena cylindrica, Anabaenopsis circularis, Rhodospirillum rubrum, and Azotobacter vinelandii. Rates of acetylene reduction showed a gradual increase and reached a peak after 2 to 6 h of continuous...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.6.640-646.1977

    authors: David KA,Fay P

    更新日期:1977-12-01 00:00:00

  • Chemotaxis to oligopeptides by Pseudomonas aeruginosa.

    abstract::A number of peptides were evaluated as chemoattractants for Pseudomonas aeruginosa. Several strains recognized tri-, tetra-, penta-, and hexapeptides in a capillary tube assay. Tripeptides altered at the carboxyl terminus were good attractants, whereas tripeptides altered at the amino terminus did not serve as chemoat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.1.363-367.1994

    authors: Kelly-Wintenberg K,Montie TC

    更新日期:1994-01-01 00:00:00

  • Bioassay and dose measurement in UV disinfection.

    abstract::A bioassay method was developed to measure the average intensity within a UV disinfection reactor. The survival of spores of Bacillus subtilis was determined as a function of UV dose to prepare a standard curve. Spores were added to unknown systems, and the survival rate was used to determine the average intensity. A ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.872-877.1983

    authors: Qualls RG,Johnson JD

    更新日期:1983-03-01 00:00:00

  • Influence of Manganese on Growth of a Sheathless Strain of Leptothrix discophora.

    abstract::Mn exerted various effects on the growth of Leptothrix discophora strain SS-1 in batch cultures depending on the concentration added to the medium. Concentrations of 0.55 to 5.5 muM Mn, comparable to those in the environment from which strain SS-1 was isolated, decreased cell yield and prolonged stationary-phase survi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.556-562.1985

    authors: Adams LF,Ghiorse WC

    更新日期:1985-03-01 00:00:00

  • Isolation and characterization of Arthrobacter bacteriophages and their application to phage typing of soil arthrobacters.

    abstract::Seventeen bacteriophages, active against 19 Arthrobacter soil isolates, were isolated from concentrated samples of river water and sewage. Attempts to isolate Arthrobacter bacteriophages from filtrates of broth cultures of the soil isolates or from ultraviolet light-irradiated cultures were unsuccessful. Bacteriophage...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.1.185-191.1978

    authors: Brown DR,Holt JG,Pattee PA

    更新日期:1978-01-01 00:00:00

  • Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction.

    abstract::Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01336-19

    authors: Campa MF,Techtmann SM,Ladd MP,Yan J,Patterson M,Garcia de Matos Amaral A,Carter KE,Ulrich N,Grant CJ,Hettich RL,Lamendella R,Hazen TC

    更新日期:2019-10-16 00:00:00

  • Genetic structure and symbiotic characteristics of a bradyrhizobium population recovered from a pasture soil.

    abstract::We examined the genetic structure and symbiotic characteristics of Bradyrhizobium isolates recovered from four legume species (Lupinus albus [white lupine], Lupinus angustifolius [blue lupine], Ornithopus compressus [yellow serradella], and Macroptilium atropurpureum [sirato]) grown in an Oregon soil. We established t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.6.1754-1761.1994

    authors: Bottomley PJ,Cheng HH,Strain SR

    更新日期:1994-06-01 00:00:00

  • Degradation of the Isoflavone Biochanin A and Its Glucoside Conjugates by Ascochyta rabiei.

    abstract::Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degrad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.1.45-48.1985

    authors: Kraft B,Barz W

    更新日期:1985-07-01 00:00:00

  • Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

    abstract::Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04028-12

    authors: Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

    更新日期:2013-05-01 00:00:00

  • D-2,3-butanediol production due to heterologous expression of an acetoin reductase in Clostridium acetobutylicum.

    abstract::Acetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions, Clostridium acetobutylicum ATCC 824 (and strains derived from it) generates both d- and l-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding A...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01616-10

    authors: Siemerink MA,Kuit W,López Contreras AM,Eggink G,van der Oost J,Kengen SW

    更新日期:2011-04-01 00:00:00

  • Thiosulfate-dependent chemolithoautotrophic growth of Bradyrhizobium japonicum.

    abstract::Thiosulfate-oxidizing sox gene homologues were found at four loci (I, II, III, and IV) on the genome of Bradyrhizobium japonicum USDA110, a symbiotic nitrogen-fixing bacterium in soil. In fact, B. japonicum USDA110 can oxidize thiosulfate and grow under a chemolithotrophic condition. The deletion mutation of the soxY(...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02783-09

    authors: Masuda S,Eda S,Ikeda S,Mitsui H,Minamisawa K

    更新日期:2010-04-01 00:00:00