Differential proteomic analysis of Rickettsia prowazekii propagated in diverse host backgrounds.

abstract：:We recently characterized a transposon-induced NaCl-sensitive mutant of Staphylococcus aureus (U. Vijaranakul, M. J. Nadakavukaren, D. O. Bayles, B. J. Wilkinson, and R. K. Jayaswal, Appl. Environ. Microbiol. 63:1889-1897, 1997). To further characterize this mutant, we determined the nucleotide sequence at the inserti...

journal_title：Applied and environmental microbiology

authors： Vijaranakul U,Xiong A,Lockwood K,Jayaswal RK

更新日期：1998-02-01 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:Micro-Raman spectroscopy is a fast and sensitive tool for the detection, classification, and identification of biological organisms. The vibrational spectrum inherently serves as a fingerprint of the biochemical composition of each bacterium and thus makes identification at the species level, or even the subspecies le...

journal_title：Applied and environmental microbiology

authors： Stöckel S,Schumacher W,Meisel S,Elschner M,Rösch P,Popp J

更新日期：2010-05-01 00:00:00

abstract：:Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate betwe...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Ast JC

更新日期：2005-02-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and to a lesser extent to the phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties and are thought to exert their toxicity by...

journal_title：Applied and environmental microbiology

authors： Dongo A,Bataillé-Simoneau N,Campion C,Guillemette T,Hamon B,Iacomi-Vasilescu B,Katz L,Simoneau P

更新日期：2009-01-01 00:00:00

abstract：:Azotobacter vinelandii OP was grown to stationary phase in defined medium. The cell-free culture medium was analyzed for cytokinin content by XAD-2 and Sephadex LH-20 chromatography, thin-layer chromatography, tobacco callus bioassay, and enzyme immunoassay. Three cytokinin-active fractions were detected and tentative...

journal_title：Applied and environmental microbiology

authors： Taller BJ,Wong TY

更新日期：1989-01-01 00:00:00

abstract：:Aquaglyceroporins (GlpFs) that transport glycerol along with water and other uncharged solutes are involved in osmoregulation in myriad species. Fungal species form a large group of eukaryotic organisms, and their GlpFs may be diverse, exhibiting various activities. However, few filamentous fungal GlpFs have been biol...

journal_title：Applied and environmental microbiology

authors： Liu XD,Wei Y,Zhou XY,Pei X,Zhang SH

更新日期：2015-10-01 00:00:00

abstract：:Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

journal_title：Applied and environmental microbiology

authors： Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

更新日期：2007-09-01 00:00:00

abstract：:A single type of bacterium, tentatively identified as a member of the genus Bacillus, was isolated from 2 of 20 midguts of Douglas fir tussock moth larvae being fed a diet of fir needles. No bacteria could be isolated from most midguts. Although spherically shaped bodies were present in the food bolus, these bodies, i...

journal_title：Applied and environmental microbiology

authors： Andrews RE,Spence KD

更新日期：1980-11-01 00:00:00

abstract：:Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce po...

journal_title：Applied and environmental microbiology

authors： McGrath JW,Quinn JP

更新日期：2000-09-01 00:00:00

abstract：:During the ripening of Gouda-type cheese, two kinds of endopeptidases were found to participate in the degradation of alphas1-CN(f1-23), a specific product from alphas1-casein hydrolyzed by chymosin. One of the endopeptidases, lactic acid bacteria endopeptidase (LEP-II), which can recognize the size of its substrates,...

journal_title：Applied and environmental microbiology

authors： Yan TR,Azuma N,Kaminogawa S,Yamauchi K

更新日期：1987-10-01 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:Penicillium decumbens is able to epoxidize cis-propenylphosphonic acid (cPA) to produce the antibiotic fosfomycin [FOM; also referred to as phosphonomycin and (-)-cis-1,2-epoxypropylphosphonic acid], a bioconversion of considerable commercial significance. We sought to improve the efficiency of the process by overexpr...

journal_title：Applied and environmental microbiology

authors： Watanabe M,Sumida N,Murakami S,Anzai H,Thompson CJ,Tateno Y,Murakami T

更新日期：1999-03-01 00:00:00

abstract：:It has recently been noted that a diversity of hyperthermophilic microorganisms have the ability to reduce Fe(III) with hydrogen as the electron donor, but the reduction of Fe(III) or other metals by these organisms has not been previously examined in detail. When Pyrobaculum islandicum was grown at 100 degrees C in a...

journal_title：Applied and environmental microbiology

authors： Kashefi K,Lovley DR

更新日期：2000-03-01 00:00:00

abstract：:Two vomitoxin-producing isolates of Fusarium spp. were grown on cracked corn for 1 to 8 weeks at 15, 20, 25, 28, and 32 degrees C. Maximum production of vomitoxin by Fusarium graminearum Schw. NRRL 5883 occurred at 30 degrees C and 40 days, and that by Fusarium roseum Schw. NRRL 6101 occurred at 26 degrees C and 41 da...

journal_title：Applied and environmental microbiology

authors： Vesonder RF,Ellis JJ,Kwolek WF,DeMarini DJ

更新日期：1982-04-01 00:00:00

abstract：:A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated that the mutant was deficient in d-xylose reductase activity that used NADPH exclusively as a cofactor, but not in activity...

journal_title：Applied and environmental microbiology

authors： Schneider H,Lee H,Barbosa Mde F,Kubicek CP,James AP

更新日期：1989-11-01 00:00:00

abstract：:The 10-mer primer OPM-01 (5'-GTT GGT GGC T-3') was used to generate random amplification of polymorphic DNA (RAPD) profiles by polymerase chain reaction for 91 strains of Listeria monocytogenes from raw milk, food, and veterinary, medical, and food-environmental sources. The profiles obtained contained 1 to 10 bands w...

journal_title：Applied and environmental microbiology

authors： Lawrence LM,Harvey J,Gilmour A

更新日期：1993-09-01 00:00:00

abstract：:Perchlorate is a known health hazard for humans, fish, and other species. Therefore, it is important to assess the response of an ecosystem exposed to perchlorate contamination. The data reported here show that a liquid chromatography-mass spectrometry-based proteomics approach for the detection of perchlorate-reducin...

journal_title：Applied and environmental microbiology

authors： Bansal R,Crawford RL,Paszczynski AJ

更新日期：2011-02-01 00:00:00

abstract：:Broad-substrate-range monooygenase enzymes, including toluene-4-monooxygenase (T4MO), can catalyze the oxidation of indole. The indole oxidation products can then condense to form the industrially important dye indigo. Site-directed mutagenesis of T4MO resulted in the creation of T4MO isoforms with altered pigment pro...

journal_title：Applied and environmental microbiology

authors： McClay K,Boss C,Keresztes I,Steffan RJ

更新日期：2005-09-01 00:00:00

abstract：:Seventeen bacteriophages, active against 19 Arthrobacter soil isolates, were isolated from concentrated samples of river water and sewage. Attempts to isolate Arthrobacter bacteriophages from filtrates of broth cultures of the soil isolates or from ultraviolet light-irradiated cultures were unsuccessful. Bacteriophage...

journal_title：Applied and environmental microbiology

authors： Brown DR,Holt JG,Pattee PA

更新日期：1978-01-01 00:00:00

abstract：:We developed a new cell surface display system in Pseudomonas putida KT2442 using OprF, an outer membrane protein of Pseudomonas aeruginosa, as an anchoring motif in a C-terminal deletion-fusion strategy. The Pseudomonas fluorescens SIK W1 lipase gene was fused to two different C-terminal truncated OprF genes, and the...

journal_title：Applied and environmental microbiology

authors： Lee SH,Lee SY,Park BC

更新日期：2005-12-01 00:00:00

abstract：:Microbial reductive dechlorination of [1,2-14C]trichloroethene to [14C]cis-dichloroethene and [14C]vinyl chloride was observed at 4 degrees C in anoxic microcosms prepared with cold temperature-adapted aquifer and river sediments from Alaska. Microbial anaerobic oxidation of [1,2-14C]cis-dichloroethene and [1,2-14C]vi...

journal_title：Applied and environmental microbiology

authors： Bradley PM,Richmond S,Chapelle FH

更新日期：2005-10-01 00:00:00

abstract：:Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, sep...

journal_title：Applied and environmental microbiology

authors： Lehman RM,O'Connell SP

更新日期：2002-04-01 00:00:00

abstract：:Mortierella isabellina ATCC 38063 is a zygomycete capable of hydroxylating fish-toxic resin acids which occur in certain pulp mill effluents to nontoxic metabolites. Addition of dehydroabietic acid (1) (80 mg/liter) to a freshly inoculated culture of M. isabellina in dextrose-yeast extract broth resulted in precursor ...

journal_title：Applied and environmental microbiology

authors： Kutney JP,Choi LS,Hewitt GM,Salisbury PJ,Singh M

更新日期：1985-01-01 00:00:00

abstract：:The subsurface of a tidal-flat sediment was analyzed down to 360 cm in depth by molecular and geochemical methods. A community structure analysis of all three domains of life was performed using domain-specific PCR followed by denaturing gradient gel electrophoresis analysis and sequencing of characteristic bands. The...

journal_title：Applied and environmental microbiology

authors： Wilms R,Sass H,Köpke B,Köster J,Cypionka H,Engelen B

更新日期：2006-04-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

journal_title：Applied and environmental microbiology

authors： Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

更新日期：1987-05-01 00:00:00

abstract：:We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Apajalahti JH,Salkinoja-Salonen MS

更新日期：1988-03-01 00:00:00

abstract：:Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precisio...

journal_title：Applied and environmental microbiology

authors： Walker M,Redelman D

更新日期：2004-03-01 00:00:00