Abstract:
:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed Tn5 in unique restriction sites. Agg mutants colonized root surfaces of seedlings grown from inoculated seeds, but at levels lower than those observed with the Agg parent. In short-term binding studies, Agg cells adhered at levels that were 20- to 30-fold less than those for Agg parental cells. These data suggest that the agglutination interaction plays a role in the attachment of P. putida to root surfaces.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Anderson AJ,Habibzadegah-Tari P,Tepper CSdoi
10.1128/AEM.54.2.375-380.1988keywords:
subject
Has Abstractpub_date
1988-02-01 00:00:00pages
375-80issue
2eissn
0099-2240issn
1098-5336journal_volume
54pub_type
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