Precipitation of iron on the surface of Leptospira interrogans is associated with mutation of the stress response metalloprotease HtpX.

abstract：:Because of high diurnal water quality fluctuations in raw municipal wastewater, the use of proportional autosampling over a period of 24 h at municipal wastewater treatment plants (WWTPs) to evaluate carbon, nitrogen, and phosphorus removal has become a standard in many countries. Microbial removal or load estimation ...

journal_title：Applied and environmental microbiology

authors： Mayer RE,Vierheilig J,Egle L,Reischer GH,Saracevic E,Mach RL,Kirschner AK,Zessner M,Sommer R,Farnleitner AH

更新日期：2015-08-01 00:00:00

abstract：:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, req...

journal_title：Applied and environmental microbiology

authors： Yung PT,Ponce A

更新日期：2008-12-01 00:00:00

abstract：:There are a number of genetic tools available for studying Francisella tularensis, the etiological agent of tularemia; however, there is no effective inducible or repressible gene expression system. Here, we describe inducible and repressible gene expression systems for F. tularensis based on the Tet repressor, TetR. ...

journal_title：Applied and environmental microbiology

authors： LoVullo ED,Miller CN,Pavelka MS Jr,Kawula TH

更新日期：2012-10-01 00:00:00

abstract：:Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or L-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with D-alaninol. To investigate the hypothesis that L-alaninol is an intermediate in the degradation of isopropylamine, two mini-Tn5 mutants unable to utilize both isopropylami...

journal_title：Applied and environmental microbiology

authors： de Azevedo Wäsch SI,van der Ploeg JR,Maire T,Lebreton A,Kiener A,Leisinger T

更新日期：2002-05-01 00:00:00

abstract：:Variations in the thymidine incorporation rate, bacterial abundance, and mean cell volumes in the surface water (0.5 m) of the Baltic Sea in spring and summer were compared in studies with different spatial scales (570 nautical miles [nmi] [ca. 1056 km], 220 nmi [ca. 407 km], 24 nmi [ca. 44 km], 12 nmi [ca. 22 km], an...

journal_title：Applied and environmental microbiology

authors： Heinänen A,Kuparinen J

更新日期：1991-11-01 00:00:00

abstract：:A sedimentary bacterial isolate has been shown to contain trans-monounsaturated fatty acids (6% of the total fatty acids). The ratio of trans- to cis-acids in this isolate was in the range 3.2 to 7.6. The identification of trans-monounsaturated acids in a marine bacterium implied that the trans-acids which have been r...

journal_title：Applied and environmental microbiology

authors： Gillan FT,Johns RB,Verheyen TV,Volkman JK,Bavor HJ

更新日期：1981-04-01 00:00:00

abstract：:After treatment of a dispersed suspension of echovirus with HOCl, much of the lost plaque titer was restored if the treated virus was induced to aggregate by adjustment of the suspending medium to pH 4.5. This did not appear to be a repair of individual virions but rather a special kind of multiplicity-related increas...

journal_title：Applied and environmental microbiology

authors： Young DC,Sharp DG

更新日期：1979-04-01 00:00:00

abstract：:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

journal_title：Applied and environmental microbiology

authors： Cleveland TE,Lax AR,Lee LS,Bhatnagar D

更新日期：1987-07-01 00:00:00

abstract：:Streptococcus thermophilus is one of the most widely used lactic acid bacteria in the dairy industry, in particular in yoghurt manufacture, where it is associated with Lactobacillus delbrueckii subsp. bulgaricus. This bacterial association, known as a proto-cooperation, is poorly documented at the molecular and regula...

journal_title：Applied and environmental microbiology

authors： Herve-Jimenez L,Guillouard I,Guedon E,Boudebbouze S,Hols P,Monnet V,Maguin E,Rul F

更新日期：2009-04-01 00:00:00

abstract：:Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

journal_title：Applied and environmental microbiology

authors： Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

更新日期：2011-08-01 00:00:00

abstract：:The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels ...

journal_title：Applied and environmental microbiology

authors： Cruz CD,Hedderley D,Fletcher GC

更新日期：2015-04-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:The genomes of 10 Aeromonas isolates identified and designated Aeromonas hydrophila WI, Riv3, and NF1 to NF4; A. dhakensis SSU; A. jandaei Riv2; and A. caviae NM22 and NM33 were sequenced and annotated. Isolates NF1 to NF4 were from a patient with necrotizing fasciitis (NF). Two environmental isolates (Riv2 and -3) we...

journal_title：Applied and environmental microbiology

authors： Grim CJ,Kozlova EV,Ponnusamy D,Fitts EC,Sha J,Kirtley ML,van Lier CJ,Tiner BL,Erova TE,Joseph SJ,Read TD,Shak JR,Joseph SW,Singletary E,Felland T,Baze WB,Horneman AJ,Chopra AK

更新日期：2014-07-01 00:00:00

abstract：:We have engineered Escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the C₁₁ to C₁₅ (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we re...

journal_title：Applied and environmental microbiology

authors： Goh EB,Baidoo EE,Keasling JD,Beller HR

更新日期：2012-01-01 00:00:00

abstract：:Microorganisms associated with the stems and roots of nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans [Glycine max (L.) Merril] were analyzed by ribosomal intergenic transcribed spacer analysis (RISA) and automated RISA (ARISA). RISA of stem samples detected no bands specific...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Rallos LE,Okubo T,Eda S,Inaba S,Mitsui H,Minamisawa K

更新日期：2008-09-01 00:00:00

abstract：:The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a sclerotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zo...

journal_title：Applied and environmental microbiology

authors： Wicklow DT,Joshi BK,Gamble WR,Gloer JB,Dowd PF

更新日期：1998-11-01 00:00:00

abstract：:Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

journal_title：Applied and environmental microbiology

authors： Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

更新日期：1989-08-01 00:00:00

abstract：:Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

journal_title：Applied and environmental microbiology

authors： Maisuria VB,Hosseinidoust Z,Tufenkji N

更新日期：2015-06-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:The pediocin-like bacteriocins, produced by lactic acid bacteria, are bactericidal polypeptides with very similar primary structures. Peptide synthesis followed by reverse-phase and ion-exchange chromatographies yielded biologically active pediocin-like bacteriocins in amounts and with a purity sufficient for characte...

journal_title：Applied and environmental microbiology

authors： Fimland G,Blingsmo OR,Sletten K,Jung G,Nes IF,Nissen-Meyer J

更新日期：1996-09-01 00:00:00

abstract：:Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

journal_title：Applied and environmental microbiology

authors： Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

更新日期：2009-01-01 00:00:00

abstract：:In contrast to extensive studies of phosphorus, widely considered the main nutrient limiting phytoplankton biomass in freshwater ecosystems, there have been few studies on the role of nitrogen in controlling phytoplankton populations. This situation may be due partly to the complexity in estimating its utilization and...

journal_title：Applied and environmental microbiology

authors： Gillor O,Harush A,Hadas O,Post AF,Belkin S

更新日期：2003-03-01 00:00:00

abstract：:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

journal_title：Applied and environmental microbiology

authors： Kingsley MT,Bohlool BB

更新日期：1992-04-01 00:00:00

abstract：:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The v...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Fouz B,Alcaide E,Amaro C

更新日期：1996-03-01 00:00:00

abstract：:Nitrification in chloraminated drinking water can have a number of adverse effects on water quality, including a loss of total chlorine and ammonia-N and an increase in the concentration of heterotrophic plate count bacteria and nitrite. To understand how nitrification develops, a study was conducted to examine the fa...

journal_title：Applied and environmental microbiology

authors： Wolfe RL,Lieu NI,Izaguirre G,Means EG

更新日期：1990-02-01 00:00:00

abstract：:The codon-optimized genes crtB and crtI of Pantoea ananatis were expressed in Yarrowia lipolytica under the control of the TEF1 promoter of Y. lipolytica. Additionally, the rate-limiting genes for isoprenoid biosynthesis in Y. lipolytica, GGS1 and HMG1, were overexpressed to increase the production of lycopene. All of...

journal_title：Applied and environmental microbiology

authors： Matthäus F,Ketelhot M,Gatter M,Barth G

更新日期：2014-03-01 00:00:00

abstract：:The effects of preexposure of soybean (Glycine max L. Merrill) roots to Rhizobium japonicum strains and subsequent establishment of other strains in the nodules were investigated by using combinations of effective strains (USDA 110 and USDA 138) and effective-ineffective strains (USDA 110 and SM-5). Strain USDA 110 wa...

journal_title：Applied and environmental microbiology

authors： Kosslak RM,Bohlool BB,Dowdle S,Sadowsky MJ

更新日期：1983-10-01 00:00:00

abstract：:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

journal_title：Applied and environmental microbiology

authors： Sembries S,Crawford RL

更新日期：1997-05-01 00:00:00

abstract：:We investigated the genetic diversity and symbiotic efficiency of 223 Sinorhizobium sp. isolates sampled from a single Mediterranean soil and trapped with four Medicago truncatula lines. DNA molecular polymorphism was estimated by capillary electrophoresis-single-stranded conformation polymorphism and restriction frag...

journal_title：Applied and environmental microbiology

authors： Rangin C,Brunel B,Cleyet-Marel JC,Perrineau MM,Béna G

更新日期：2008-09-01 00:00:00

abstract：:Three strains of Streptococcus bovis, a homolactic bacterium capable of utilizing starch, were evaluated for growth kinetics and ability to decrease the pH of alfalfa silage. A selected strain was evaluated for its competitiveness as an inoculant with Enterococcus faecium, an organism used in inoculants, and for its a...

journal_title：Applied and environmental microbiology

authors： Jones BA,Muck RE,Ricke SC

更新日期：1991-10-01 00:00:00