TetR-based gene regulation systems for Francisella tularensis.

abstract：:A genetic transformation system with similarities to those reported for gram-negative bacteria was found to be associated with membrane vesicles of the ruminal cellulolytic genus Ruminococcus. Double-stranded DNA was recovered from the subcellular particulate fraction of all the cellulolytic ruminococci examined. Elec...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Yokoyama MT,Forster RJ,Ouwerkerk D,Bain PA,Mawhinney EL

更新日期：2005-08-01 00:00:00

abstract：:A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential...

journal_title：Applied and environmental microbiology

authors： Quéméneur M,Heinrich-Salmeron A,Muller D,Lièvremont D,Jauzein M,Bertin PN,Garrido F,Joulian C

更新日期：2008-07-01 00:00:00

abstract：:Members of the genus Clavibacter are economically important bacterial plant pathogens infecting a set of diverse agricultural crops (e.g., alfalfa, corn, potato, tomato, and wheat). Tomato-associated Clavibacter sp. strains account for a great portion of the genetic diversity of the genus, and C. michiganensissensu st...

journal_title：Applied and environmental microbiology

authors： Osdaghi E,Rahimi T,Taghavi SM,Ansari M,Zarei S,Portier P,Briand M,Jacques MA

更新日期：2020-03-02 00:00:00

abstract：:Here, we report the genome of one gammaproteobacterial member of the gut microbiota, for which we propose the name "Candidatus Schmidhempelia bombi," that was inadvertently sequenced alongside the genome of its host, the bumble bee, Bombus impatiens. This symbiont is a member of the recently described bacterial order ...

journal_title：Applied and environmental microbiology

authors： Martinson VG,Magoc T,Koch H,Salzberg SL,Moran NA

更新日期：2014-07-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...

journal_title：Applied and environmental microbiology

authors： Kumagai T,Ozawa T,Tanimoto M,Noda M,Matoba Y,Sugiyama M

更新日期：2015-11-01 00:00:00

abstract：:Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

journal_title：Applied and environmental microbiology

authors： Beltran EC,Neilan BA

更新日期：2000-10-01 00:00:00

abstract：:Sakacin K is an antilisterial bacteriocin produced by Lactobacillus sake CTC 494, a strain isolated from Spanish dry fermented sausages. The biokinetics of cell growth and bacteriocin production of L. sake CTC 494 in vitro during laboratory fermentations were investigated by making use of MRS broth. The data obtained ...

journal_title：Applied and environmental microbiology

authors： Leroy F,de Vuyst L

更新日期：1999-03-01 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a st...

journal_title：Applied and environmental microbiology

authors： Caruso C,Rizzo C,Mangano S,Poli A,Di Donato P,Finore I,Nicolaus B,Di Marco G,Michaud L,Lo Giudice A

更新日期：2018-01-31 00:00:00

abstract：:The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

journal_title：Applied and environmental microbiology

authors： Mermelstein LD,Papoutsakis ET

更新日期：1993-04-01 00:00:00

abstract：:Bacteria in the genus Streptomyces and its close relatives are prolific producers of secondary metabolites with antibiotic activity. Genome sequencing of these bacteria has revealed a rich source of potentially new antibiotic pathways, whose products have never been observed. Moreover, these new pathways can provide n...

journal_title：Applied and environmental microbiology

authors： Fayed B,Ashford DA,Hashem AM,Amin MA,El Gazayerly ON,Gregory MA,Smith MC

更新日期：2015-12-01 00:00:00

abstract：:The pathogenicity of Listeria monocytogenes as influenced by growth on crabmeat at 5 and 10 degrees C was studied. Crabmeat was inoculated with L. monocytogenes V7 (ca. 10(4) CFU/g) and incubated for up to 14 days at 5 and 10 degrees C. At selected incubation times, L. monocytogenes was removed from crabmeat by washin...

journal_title：Applied and environmental microbiology

authors： Brackett RE,Beuchat LR

更新日期：1990-05-01 00:00:00

abstract：:The biological utilization of CO(2) and H(2) for the formation of short-chain fatty acids was studied by using a mixed culture of bacteria. Optimization of a medium was carried out in continuous culture to identify limiting factors which controlled growth and production of organic acids. The optimal pH for growth and ...

journal_title：Applied and environmental microbiology

authors： Goldberg I,Cooney CL

更新日期：1981-01-01 00:00:00

abstract：:Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...

journal_title：Applied and environmental microbiology

authors： Voordouw G,Armstrong SM,Reimer MF,Fouts B,Telang AJ,Shen Y,Gevertz D

更新日期：1996-05-01 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...

journal_title：Applied and environmental microbiology

authors： Repizo GD,Blancato VS,Mortera P,Lolkema JS,Magni C

更新日期：2013-05-01 00:00:00

abstract：:An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 20...

journal_title：Applied and environmental microbiology

authors： Anglès d'Auriac MB,Roberts H,Shaw T,Sirevåg R,Hermansen LF,Berg JD

更新日期：2000-10-01 00:00:00

abstract：:The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflamma...

journal_title：Applied and environmental microbiology

authors： Wang Z,Wang J,Cheng Y,Liu X,Huang Y

更新日期：2011-11-01 00:00:00

abstract：:The thermoacidophile Acidianus is widely distributed in Yellowstone National Park hot springs that span large gradients in pH (1.60 to 4.84), temperature (42 to 90°C), and mineralogical composition. To characterize the potential role of flexibility in mineral-dependent energy metabolism in contributing to the widespre...

journal_title：Applied and environmental microbiology

authors： Amenabar MJ,Boyd ES

更新日期：2018-05-31 00:00:00

abstract：:Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

journal_title：Applied and environmental microbiology

authors： Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

更新日期：2018-02-14 00:00:00

abstract：:Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting...

journal_title：Applied and environmental microbiology

authors： Pugin B,Cornejo FA,Muñoz-Díaz P,Muñoz-Villagrán CM,Vargas-Pérez JI,Arenas FA,Vásquez CC

更新日期：2014-11-01 00:00:00

abstract：:Bacterial source tracking is used to apportion fecal pollution among putative sources. Within this context, library-independent markers are genetic or phenotypic traits that can be used to identify the host origin without a need for library-dependent classification functions. The objective of this project was to use m...

journal_title：Applied and environmental microbiology

authors： Soule M,Kuhn E,Loge F,Gay J,Call DR

更新日期：2006-03-01 00:00:00

abstract：:Electrophoretic karyotypes (EKs) of 83 isolates were variable within agricultural and natural populations of Sclerotinia sclerotiorum, as well as among S. sclerotiorum, Sclerotinia minor, and Sclerotinia trifoliorum. Variation in EKs was not observed within six mitotic or three meiotic lineages of isolates. EKs of 8 t...

journal_title：Applied and environmental microbiology

authors： Errampalli D,Kohn LM

更新日期：1996-11-01 00:00:00

abstract：:We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we la...

journal_title：Applied and environmental microbiology

authors： Guglielmetti S,Tamagnini I,Mora D,Minuzzo M,Scarafoni A,Arioli S,Hellman J,Karp M,Parini C

更新日期：2008-08-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:Most Shiga toxin-producing Escherichia coli (STEC) strains associated with severe disease, such as hemolytic-uremic syndrome (HUS), carry large enterohemolysin-encoding (ehxA) plasmids, e.g., pO157 and pO103, that contribute to STEC clinical manifestations. Six ehxA subtypes (A through F) exist that phylogenetically c...

journal_title：Applied and environmental microbiology

authors： Lorenz SC,Monday SR,Hoffmann M,Fischer M,Kase JA

更新日期：2016-10-14 00:00:00

abstract：:The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...

journal_title：Applied and environmental microbiology

authors： Martinez-Urtaza J,Saco M,de Novoa J,Perez-Piñeiro P,Peiteado J,Lozano-Leon A,Garcia-Martin O

更新日期：2004-04-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00