Abstract:
:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of all the bacteria tested. No L-methionine deaminase activity could be detected in any of the ripening bacteria and L-methionine aminotransferase was detected in CFEs of Brevibacterium linens, Micrococcus luteus, and Corynebacterium glutamicum. The results suggest that several pathways for L-methionine catabolism probably coexist in these ripening bacteria.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Bonnarme P,Psoni L,Spinnler HEdoi
10.1128/aem.66.12.5514-5517.2000keywords:
subject
Has Abstractpub_date
2000-12-01 00:00:00pages
5514-7issue
12eissn
0099-2240issn
1098-5336journal_volume
66pub_type
杂志文章abstract::Chlorpromazine was used to perform a test for the detection of microbial peroxidase activities. The compound acts as both a cell permeabilizer and a reagent in the procedure developed which allows the detection of peroxidase and peroxidase like reactions both semiquantitatively in whole cell determinations and quantit...
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journal_title:Applied and environmental microbiology
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