Purification and characterization of a novel antibacterial protein from the marine bacterium D2.


:A biofilm-forming marine bacterium, D2, isolated from the surface of the tunicate Ciona intestinalis, was found to produce a novel, 190-kDa protein with antibacterial activity. The protein contained at least two subunits of 60 and 80 kDa, joined together by noncovalent bonds, and was shown to be released by D2 cells into the surrounding medium during stationary phase. N-terminal sequence analysis revealed no close similarity of this protein to any other proteins within the Swiss Prot database. Bacteriocidal activity against a wide variety of marine and medical bacterial isolates was observed, 77% of the strains tested being sensitive to the protein. Bacterial strains varied in their resistance to the D2 protein, with D2 itself being among the most sensitive with an MBC in liquid suspension of 4 micrograms/ml. An apparent increased resistance of D2 to the protein as the cells progressed further into stationary phase was observed and seen as a possible explanation for its survival despite the production of an autoinhibitory factor. The ability of the D2 bacterium to produce an antibacterial factor in addition to its inhibitory effects on marine invertebrates and algae (S. Egan et al., unpublished data) indicates that D2 has the potential to greatly affect the survival of a range of colonizers of the marine surface environment.


Appl Environ Microbiol


James SG,Holmström C,Kjelleberg S




Has Abstract


1996-08-01 00:00:00












  • Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates.

    abstract::Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

    更新日期:1988-12-01 00:00:00

  • Parallel Evolution of Two Clades of an Atlantic-Endemic Pathogenic Lineage of Vibrio parahaemolyticus by Independent Acquisition of Related Pathogenicity Islands.

    abstract::Shellfish-transmitted Vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the Northeast United States. This change coincided with a bacterial population shift toward human-pathogenic variants occurring in part through the introduction of several Pa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Xu F,Gonzalez-Escalona N,Drees KP,Sebra RP,Cooper VS,Jones SH,Whistler CA

    更新日期:2017-08-31 00:00:00

  • Enhancing nitrite inhibition of Clostridium botulinum with isoascorbate in perishable canned cured meat.

    abstract::Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Tompkin RB,Christiansen LN,Shaparis AB

    更新日期:1978-01-01 00:00:00

  • Occurrence and molecular characteristics of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in an academic veterinary hospital.

    abstract::Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of eac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Ishihara K,Shimokubo N,Sakagami A,Ueno H,Muramatsu Y,Kadosawa T,Yanagisawa C,Hanaki H,Nakajima C,Suzuki Y,Tamura Y

    更新日期:2010-08-01 00:00:00

  • Free-living Rhizobium strain able to grow on n(2) as the sole nitrogen source.

    abstract::A Rhizobium strain isolated from stem nodules of the legume Sesbania rostrata was shown to grow on atmospheric nitrogen (N(2)) as the sole nitrogen source. Non-N(2)-fixing mutants isolated directly on agar plates formed nodules that did not fix N(2) when inoculated into the host plant. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Dreyfus BL,Elmerich C,Dommergues YR

    更新日期:1983-02-01 00:00:00

  • Phylogenetic characterization of a polychlorinated-dioxin- dechlorinating microbial community by use of microcosm studies.

    abstract::Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the ac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Yoshida N,Takahashi N,Hiraishi A

    更新日期:2005-08-01 00:00:00

  • Simplified X-ray film method for detection of bacterial volatilization of mercury chloride by Escherichia coli.

    abstract::A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Nakamura K,Nakahara H

    更新日期:1988-11-01 00:00:00

  • Moderate prevalence of antimicrobial resistance in Escherichia coli isolates from lettuce, irrigation water, and soil.

    abstract::Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

    更新日期:2013-11-01 00:00:00

  • Variation of microcystins, cyanobacterial hepatotoxins, in Anabaena spp. as a function of growth stimuli.

    abstract::Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rapala J,Sivonen K,Lyra C,Niemelä SI

    更新日期:1997-06-01 00:00:00

  • Dynamics of Photosynthesis in a Glycogen-Deficient glgC Mutant of Synechococcus sp. Strain PCC 7002.

    abstract::Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

    更新日期:2015-09-01 00:00:00

  • Multiple Environmental Factors Influence the Importance of the Phosphodiesterase DipA upon Pseudomonas aeruginosa Swarming.

    abstract::Pseudomonas aeruginosa exhibits flagellum-mediated swimming in liquid and swarming on hydrated surfaces under diverse nutrient conditions. Prior studies have implicated a phosphodiesterase, DipA, in regulating these flagellum-mediated motilities, but collectively, the necessity for DipA was unclear. In this study, we ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Mattingly AE,Kamatkar NG,Morales-Soto N,Borlee BR,Shrout JD

    更新日期:2018-03-19 00:00:00

  • Effect of parental growth on dynamics of conjugative plasmid transfer in the pea spermosphere.

    abstract::Plasmid transfer rates for the conjugative plasmid R388::Tn1721 from Pseudomonas cepacia (donor) to Pseudomonas fluorescens (recipient) on agar media, in broth, and in microcosms containing sterile or nonsterile soil, in the presence or absence of germinating pea seeds, were determined. Donors, recipients, and transco...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Sudarshana P,Knudsen GR

    更新日期:1995-08-01 00:00:00

  • Degradation of n-haloalkanes and alpha, omega-dihaloalkanes by wild-type and mutants of Acinetobacter sp. strain GJ70.

    abstract::A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an induci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Janssen DB,Jager D,Witholt B

    更新日期:1987-03-01 00:00:00

  • Molecular characterization of commensal Escherichia coli adapted to different compartments of the porcine gastrointestinal tract.

    abstract::The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

    更新日期:2012-10-01 00:00:00

  • Regio-selective 10-hydroxylation of patchoulol, a sesquiterpene, by pithomyces species.

    abstract::Of some 350 microorganisms screened, four strains of Pithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol: Pithomyces sp. NRJ201, P. chartarum NRJ210, and, to a lesser extent, P. cynodontis ATCC 26150 and P. atro-olivaceus IFO 6651 were found ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Suhara Y,Itoh S,Ogawa M,Yokose K,Sawada T,Sano T,Ninomiya R,Maruyama HB

    更新日期:1981-08-01 00:00:00

  • Comparison of pmoA PCR primer sets as tools for investigating methanotroph diversity in three Danish soils.

    abstract::Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bourne DG,McDonald IR,Murrell JC

    更新日期:2001-09-01 00:00:00

  • Transfer of Enteric Viruses Adenovirus and Coxsackievirus and Bacteriophage MS2 from Liquid to Human Skin.

    abstract::Indirect exposure to waterborne viruses increases the risk of infection, especially among children with frequent hand-to-mouth contacts. Here, we quantified the transfer of one bacteriophage (MS2) and two enteric viruses (adenovirus and coxsackievirus) from liquid to skin. MS2, a commonly used enteric virus surrogate,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pitol AK,Bischel HN,Boehm AB,Kohn T,Julian TR

    更新日期:2018-10-30 00:00:00

  • Transcriptional Profile during Deoxycholate-Induced Sporulation in a Clostridium perfringens Isolate Causing Foodborne Illness.

    abstract:UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Yasugi M,Okuzaki D,Kuwana R,Takamatsu H,Fujita M,Sarker MR,Miyake M

    更新日期:2016-05-02 00:00:00

  • Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences.

    abstract::The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Stahl DA,Lane DJ,Olsen GJ,Pace NR

    更新日期:1985-06-01 00:00:00

  • Model organisms retain an "ecological memory" of complex ecologically relevant environmental variation.

    abstract::Although tractable model organisms are essential to characterize the molecular mechanisms of evolution and adaptation, the ecological relevance of their behavior is not always clear because certain traits are easily lost during long-term laboratory culturing. Here, we demonstrate that despite their long tenure in the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Beer KD,Wurtmann EJ,Pinel N,Baliga NS

    更新日期:2014-03-01 00:00:00

  • Involvement of acyl coenzyme A oxidase isozymes in biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica.

    abstract::We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lacto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Waché Y,Laroche C,Bergmark K,Møller-Andersen C,Aguedo M,Le Dall MT,Wang H,Nicaud JM,Belin JM

    更新日期:2000-03-01 00:00:00

  • Stable-Isotope Analysis of a Combined Nitrification-Denitrification Sustained by Thermophilic Methanotrophs under Low-Oxygen Conditions.

    abstract::To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) deco...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pel R,Oldenhuis R,Brand W,Vos A,Gottschal JC,Zwart KB

    更新日期:1997-02-01 00:00:00

  • Application of a specific and sensitive radiometric assay for microbial lipase activities in marine water samples from the lagoon of nouméa.

    abstract::Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

    更新日期:2003-12-01 00:00:00

  • Field evaluation of a semiautomated method for rapid and simple analysis of recreational water microbiological quality.

    abstract::An early warning system using a rapid enzymatic semiautomated method suitable for fecal coliform detection in recreational waters within 8 h was developed further and evaluated in this study. This rapid method was compared to the standard method followed in the United Kingdom. We used 1,011 samples originating from 20...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Anglès d'Auriac MB,Roberts H,Shaw T,Sirevåg R,Hermansen LF,Berg JD

    更新日期:2000-10-01 00:00:00

  • Enzymatic Biosynthesis of Novel Resveratrol Glucoside and Glycoside Derivatives.

    abstract::A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

    更新日期:2014-12-01 00:00:00

  • celB, a gene coding for a bifunctional cellulase from the extreme thermophile "Caldocellum saccharolyticum".

    abstract::"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

    更新日期:1990-10-01 00:00:00

  • Prevalence of the Chloroflexi-related SAR202 bacterioplankton cluster throughout the mesopelagic zone and deep ocean.

    abstract::Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subg...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Morris RM,Rappé MS,Urbach E,Connon SA,Giovannoni SJ

    更新日期:2004-05-01 00:00:00

  • Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regions.

    abstract::Salmonella species are pathogenic bacteria often detected in sewage, freshwater, marine coastal water, and groundwater. Salmonella spp. can survive for long periods in natural waters, and the persistence of specific and epidemic strains is of great concern in public health. However, the diversity of species found in t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Baudart J,Lemarchand K,Brisabois A,Lebaron P

    更新日期:2000-04-01 00:00:00

  • Immunoquantitative real-time PCR for detection and quantification of Staphylococcus aureus enterotoxin B in foods.

    abstract::A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

    更新日期:2006-10-01 00:00:00

  • Oxidation of ethylene glycol by a salt-requiring bacterium.

    abstract::Bacterium T-52, cultured on ethylene glycol, readily oxidized glycolate and glyoxylate and exhibited elevated activities of ethylene glycol dehydrogenase and glycolate oxidase. Labeled glyoxylate was identified in reaction mixtures containing [C]-ethylene glycol, but no glycolate was detected. The most likely pathway ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章


    authors: Caskey WH,Taber WA

    更新日期:1981-07-01 00:00:00