Anaerobic degradation of propionate by a mesophilic acetogenic bacterium in coculture and triculture with different methanogens.

abstract：:We have isolated and characterized the cDNA and genomic DNA coding for a phenoloxidase, laccase I, previously purified from culture supernatant of the newly isolated ligninolytic basidiomycete PM1 (CECT 2971). A cDNA library from basidiomycete PM1 was constructed, and laccase-encoding cDNAs were identified by screenin...

journal_title：Applied and environmental microbiology

authors： Coll PM,Tabernero C,Santamaría R,Pérez P

更新日期：1993-12-01 00:00:00

abstract：:Equations defining the reaction of microbial alginase on commercial sodium alginate are presented with respect to the viscometric assay of enzyme activity. The negative log of K was found to be linearly related to the concentration of algin, where K is defined as the change in the reciprocal of relative viscosity with...

journal_title：Applied and environmental microbiology

authors： Stevens RA,Levin RE

更新日期：1976-06-01 00:00:00

abstract：:As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...

journal_title：Applied and environmental microbiology

authors： Yates MV,Ouyang Y

更新日期：1992-05-01 00:00:00

abstract：:Moistened barley was inoculated with 1.4 x 10(3) and 1.4 x 10(5) spores, respectively, from ochratoxin A-producing strains of Aspergillus ochraceus and Penicillium varidicatum. To estimate fungal tissue in the barley, the amount of glucosamine was followed for 28 days at 10 and 25 degrees C. Ochratoxin A was also foll...

journal_title：Applied and environmental microbiology

authors： Häggblom P

更新日期：1982-05-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...

journal_title：Applied and environmental microbiology

authors： Field KG,Gordon D,Wright T,Rappé M,Urback E,Vergin K,Giovannoni SJ

更新日期：1997-01-01 00:00:00

abstract：:Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...

journal_title：Applied and environmental microbiology

authors： Anderson AJ,Habibzadegah-Tari P,Tepper CS

更新日期：1988-02-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00

abstract：:A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the...

journal_title：Applied and environmental microbiology

authors： Rosenberg M

更新日期：1981-08-01 00:00:00

abstract：:Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

journal_title：Applied and environmental microbiology

authors： Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

更新日期：2014-02-01 00:00:00

abstract：:Lactobacillus iners is often associated with vaginal dysbiosis and bacterial vaginosis (BV), which are risk factors for adverse gynecological and obstetric outcomes. To discover natural inhibitors of L. iners, cell-free culture supernatants (CFSs) from 77 vaginal human Lactobacillus strains and 1 human intestinal stra...

journal_title：Applied and environmental microbiology

authors： Nilsen T,Swedek I,Lagenaur LA,Parks TP

更新日期：2020-10-01 00:00:00

abstract：:"Candidatus Nitrosotenuis uzonensis" is the only cultured moderately thermophilic member of the thaumarchaeotal order Nitrosopumilales (NP) that contains many mesophilic marine strains. We examined its membrane lipid composition at different growth temperatures (37°C, 46°C, and 50°C). Its lipids were all membrane-span...

journal_title：Applied and environmental microbiology

authors： Bale NJ,Palatinszky M,Rijpstra WIC,Herbold CW,Wagner M,Sinninghe Damsté JS

更新日期：2019-10-01 00:00:00

abstract：:Several members of the glycoside hydrolase 61 (GH61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. However, purified GH61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignaci...

journal_title：Applied and environmental microbiology

authors： Langston JA,Shaghasi T,Abbate E,Xu F,Vlasenko E,Sweeney MD

更新日期：2011-10-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:Bacterial aggregates from a chemolithoautotrophic, nitrifying fluidized bed reactor were investigated with microsensors and rRNA-based molecular techniques. The microprofiles of O2, NH4+, NO2-, and NO3- demonstrated the occurrence of complete nitrification in the outer 125 microgram of the aggregates. The ammonia oxid...

journal_title：Applied and environmental microbiology

authors： Schramm A,De Beer D,Wagner M,Amann R

更新日期：1998-09-01 00:00:00

abstract：:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless bet...

journal_title：Applied and environmental microbiology

authors： Binishofer B,Moll I,Henrich B,Bläsi U

更新日期：2002-08-01 00:00:00

abstract：:A toluene-degrading methanogenic consortium enriched from creosote-contaminated aquifer material was maintained on toluene as the sole carbon and energy source for 10 years. The species in the consortium were characterized by using a molecular approach. Total genomic DNA was isolated, and 16S rRNA genes were amplified...

journal_title：Applied and environmental microbiology

authors： Ficker M,Krastel K,Orlicky S,Edwards E

更新日期：1999-12-01 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

journal_title：Applied and environmental microbiology

authors： Desomer J,Dhaese P,Montagu MV

更新日期：1990-09-01 00:00:00

abstract：:Digital dermatitis (DD) is an important cause of lameness in dairy cattle worldwide. It has now been reported in beef cattle and also sheep (contagious ovine digital dermatitis [CODD]). Three Treponema phylogroups are consistently isolated from lesions, Treponema medium-like, Treponema phagedenis-like, and Treponema p...

journal_title：Applied and environmental microbiology

authors： Sullivan LE,Carter SD,Duncan JS,Grove-White DH,Angell JW,Evans NJ

更新日期：2015-11-01 00:00:00

abstract：:The biotinylating reagent succinimidyl 6-(biotinamido)hexanoate was used to label the cell surfaces of the cosmopolitan, marine, eukaryotic microorganism Emiliania huxleyi under different growth conditions. Proteins characteristic of different nutrient conditions could be identified. In particular, a nitrogen-regulate...

journal_title：Applied and environmental microbiology

authors： Palenik B,Koke JA

更新日期：1995-09-01 00:00:00

abstract：:Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are l-rhamnose and d-glucose. C. beijerinckii DSM 6423 ut...

journal_title：Applied and environmental microbiology

authors： Diallo M,Simons AD,van der Wal H,Collas F,Houweling-Tan B,Kengen SWM,López-Contreras AM

更新日期：2019-02-20 00:00:00

abstract：:Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by str...

journal_title：Applied and environmental microbiology

authors： Kojic M,Vujcic M,Banina A,Cocconcelli P,Cerning J,Topisirovic L

更新日期：1992-12-01 00:00:00

abstract：:DysI is identified as the protein that confers specific immunity to dysgalacticin, a plasmid-encoded streptococcal bacteriocin. dysI is transcribed as part of the copG-repB-dysI replication-associated operon. DysI appears to function at the membrane level to prevent the inhibitory effects of dysgalacticin on glucose t...

journal_title：Applied and environmental microbiology

authors： Swe PM,Heng NC,Cook GM,Tagg JR,Jack RW

更新日期：2010-12-01 00:00:00

abstract：:The human enteropathogen Clostridium difficile constitutes a key public health issue in industrialized countries. Many aspects of C. difficile pathophysiology and adaptation inside the host remain poorly understood. We have recently reported that this bacterium possesses an active CRISPR-Cas system of subtype I-B for ...

journal_title：Applied and environmental microbiology

authors： Maikova A,Kreis V,Boutserin A,Severinov K,Soutourina O

更新日期：2019-10-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:In the natural environment, most of the phages that target bacteria are thought to exist in biofilm ecosystems. The purpose of this study was to gain a clearer understanding of the reactivity of these viral particles when they come into contact with bacteria embedded in biofilms. Experimentally, we quantified lactococ...

journal_title：Applied and environmental microbiology

authors： Briandet R,Lacroix-Gueu P,Renault M,Lecart S,Meylheuc T,Bidnenko E,Steenkeste K,Bellon-Fontaine MN,Fontaine-Aupart MP

更新日期：2008-04-01 00:00:00

abstract：:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it t...

journal_title：Applied and environmental microbiology

authors： Egland PG,Gibson J,Harwood CS

更新日期：2001-03-01 00:00:00

abstract：:Indirect pathogenicity (IP), the commensal protection of antibiotic-sensitive pathogens by resistant microorganisms of low intrinsic virulence, can prevent the eradication of polymicrobial infections. The contributions of antibiotic resistance mechanisms and biofilm structure to IP within polymicrobial biofilms were i...

journal_title：Applied and environmental microbiology

authors： O'Connell HA,Kottkamp GS,Eppelbaum JL,Stubblefield BA,Gilbert SE,Gilbert ES

更新日期：2006-07-01 00:00:00

abstract：:The effects of pulsed electric field (PEF) treatment and processing factors on the inactivation kinetics of Listeria innocua NCTC 11289 were investigated by using a pilot plant PEF unit with a flow rate of 200 liters/h. The electric field strength, pulse length, number of pulses, and inlet temperature were the most si...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Dutreux N,Smelt JP,Lelieveld HL

更新日期：1999-12-01 00:00:00