Abstract:
:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless beta-glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei phiFSW-TI lysogen by showing that the gusA reporter gene, controlled by the isolated phiFSW-TI promoter, was repressed at 28 degrees C and expressed at 42 degrees C. Moreover, a homology search revealed that the C terminus of the isolated phiFSW repressor shows a high similarity to the small mutS-related domain of the MutS2 protein family that is unprecedented for phage-encoded repressor proteins.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Binishofer B,Moll I,Henrich B,Bläsi Udoi
10.1128/aem.68.8.4132-4135.2002keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
4132-5issue
8eissn
0099-2240issn
1098-5336journal_volume
68pub_type
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