Inducible promoter-repressor system from the Lactobacillus casei phage phiFSW.

Abstract:

:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless beta-glucuronidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of the promoter-repressor system was verified in the L. casei phiFSW-TI lysogen by showing that the gusA reporter gene, controlled by the isolated phiFSW-TI promoter, was repressed at 28 degrees C and expressed at 42 degrees C. Moreover, a homology search revealed that the C terminus of the isolated phiFSW repressor shows a high similarity to the small mutS-related domain of the MutS2 protein family that is unprecedented for phage-encoded repressor proteins.

journal_name

Appl Environ Microbiol

authors

Binishofer B,Moll I,Henrich B,Bläsi U

doi

10.1128/aem.68.8.4132-4135.2002

keywords:

subject

Has Abstract

pub_date

2002-08-01 00:00:00

pages

4132-5

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

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