Isolation and characterization of "Methanosphaera cuniculi" sp. nov.

abstract：:Over 1,400 water samples were collected biweekly over 6 years from an intermittent stream protected and unprotected from pasturing cattle. The samples were monitored for host-specific Bacteroidales markers, Cryptosporidium species/genotypes, viruses and coliphages associated with humans or animals, and bacterial zoono...

journal_title：Applied and environmental microbiology

authors： Wilkes G,Brassard J,Edge TA,Gannon V,Jokinen CC,Jones TH,Marti R,Neumann NF,Ruecker NJ,Sunohara M,Topp E,Lapen DR

更新日期：2013-10-01 00:00:00

abstract：:Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

journal_title：Applied and environmental microbiology

authors： Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

更新日期：2013-03-01 00:00:00

abstract：:Magnetotactic bacteria (MTB) form intracellular chain-assembled nanocrystals of magnetite or greigite termed magnetosomes. The characterization of magnetosome crystals requires electron microscopy due to their nanoscopic sizes. However, electron microscopy does not provide phylogenetic information for MTB. We have dev...

journal_title：Applied and environmental microbiology

authors： Li J,Zhang H,Menguy N,Benzerara K,Wang F,Lin X,Chen Z,Pan Y

更新日期：2017-05-31 00:00:00

abstract：:Conjugal transfer of lactose-fermenting ability (Lac+), nisin resistance (Nisr), and phage resistance (Hsp+) was demonstrated in matings between Streptococcus lactis ME2 (donor) and Streptococcus cremoris M43a (recipient), a derivative of M12R. Transconjugants were detected by transfer of Lac+ and were found to exhibi...

journal_title：Applied and environmental microbiology

authors： Steenson LR,Klaenhammer TR

更新日期：1985-10-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used to produce recombinant proteins, taking advantage of this species' high-density cell growth and strong ability to secrete proteins. Circular plasmids containing the P. pastoris-specific autonomously replicating sequence (PARS1) permit transformation of P. pastori...

journal_title：Applied and environmental microbiology

authors： Nakamura Y,Nishi T,Noguchi R,Ito Y,Watanabe T,Nishiyama T,Aikawa S,Hasunuma T,Ishii J,Okubo Y,Kondo A

更新日期：2018-07-17 00:00:00

abstract：:Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was i...

journal_title：Applied and environmental microbiology

authors： Wright SF

更新日期：1990-07-01 00:00:00

abstract：:The induction of hydroxyl radical (OH) production via quinone redox cycling in white-rot fungi was investigated to improve pollutant degradation. In particular, we examined the influence of 4-methoxybenzaldehyde (anisaldehyde), Mn(2+), and oxalate on Pleurotus eryngii OH generation. Our standard quinone redox cycling ...

journal_title：Applied and environmental microbiology

authors： Gómez-Toribio V,García-Martín AB,Martínez MJ,Martínez AT,Guillén F

更新日期：2009-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of whole cells of methanogens in samples from anaerobic continuously stirred tank digesters treating slurries of solid waste. The assay was found to allow for quantitative analysis of the most important groups of methanogens in samples from anaerobic...

journal_title：Applied and environmental microbiology

authors： Sørensen AH,Ahring BK

更新日期：1997-05-01 00:00:00

abstract：:An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent homogeneity from Escherichia coli K-12. The enzyme catalyzes the trans...

journal_title：Applied and environmental microbiology

authors： Schulz A,Taggeselle P,Tripier D,Bartsch K

更新日期：1990-01-01 00:00:00

abstract：:[This corrects the article on p. 662 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-06-01 00:00:00

abstract：:Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the ...

journal_title：Applied and environmental microbiology

authors： Aranda A,del Olmo ML

更新日期：2004-04-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:Four DNA probes were derived that hybridized specifically to DNA from Lactobacillus acidophilus O. The probes were constructed by randomly cloning lactobacillus DNA in plasmid vector pBR322. Two of the probes (pSR1 and pSR2) were composed of vector and plasmid DNA inserts (3.6 and 1.6 kb, respectively); the others (pS...

journal_title：Applied and environmental microbiology

authors： Rodtong S,Dobbinson S,Thode-Andersen S,McConnell MA,Tannock GW

更新日期：1993-11-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00

abstract：:Bacterial precipitation of barite (BaSO(4)) under laboratory conditions is reported for the first time. The bacterium Myxococcus xanthus was cultivated in a solid medium with a diluted solution of barium chloride. Crystallization occurred as a result of the presence of live bacteria and the bacterial metabolic activit...

journal_title：Applied and environmental microbiology

authors： González-Muñoz MT,Fernández-Luque B,Martínez-Ruiz F,Ben Chekroun K,Arias JM,Rodríguez-Gallego M,Martínez-Cañamero M,de Linares C,Paytan A

更新日期：2003-09-01 00:00:00

abstract：:Pot experiments showed that Rhizobium japonicum cells entrapped in a polyacrylamide gel could be used as an inoculant for soybeans and compared favorably to laboratory-made peat base inoculant containing the same bacterial strain. ...

journal_title：Applied and environmental microbiology

authors： Dommergues YR,Diem HG,Divies C

更新日期：1979-04-01 00:00:00

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:Cultures of Anabaena variabilis were exposed to different light intensities, and the time course of photoadaptation was measured by photosynthetic rate and changes in pigmentation. A shift down in intensity of 284 muEin . m . sec caused a temporary decrease in the photosynthetic response followed by gradual adaptation...

journal_title：Applied and environmental microbiology

authors： Collins CD,Boylen CW

更新日期：1982-07-01 00:00:00

abstract：:We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional ...

journal_title：Applied and environmental microbiology

authors： Liu BH,Chu FS

更新日期：1998-10-01 00:00:00

abstract：:To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

journal_title：Applied and environmental microbiology

authors： Shin H,Lee JH,Yoon H,Kang DH,Ryu S

更新日期：2014-01-01 00:00:00

abstract：:The volatile substances present in a eutrophic shallow lake were determined qualitatively and quantitatively during the growing season, allowing a first-time analysis of the dynamics of these compounds in a water body. The major compounds found were beta-cyclocitral, alpha-cyclocitral, beta-ionone, 1,3,3-trimethylcycl...

journal_title：Applied and environmental microbiology

authors： Jüttner F

更新日期：1984-04-01 00:00:00

abstract：:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitat...

journal_title：Applied and environmental microbiology

authors： Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

更新日期：2011-02-01 00:00:00

abstract：:Flow cytometry was used to enumerate and characterize bacteria from a sand column microcosm simulating aquifer conditions. Pure cultures of a species of Bacillus isolated from subsurface sediments or Bacillus megaterium were first evaluated to identify these organisms' characteristic histograms. Counting was then carr...

journal_title：Applied and environmental microbiology

authors： Deleo PC,Baveye P

更新日期：1996-12-01 00:00:00

abstract：UNLABELLED:Rhizobia are best known for nodulating legume roots and fixing atmospheric nitrogen for the host in exchange for photosynthates. However, the majority of the diverse strains of rhizobia do not form nodules on legumes, often because they lack key loci that are needed to induce nodulation. Nonnodulating rhizob...

journal_title：Applied and environmental microbiology

authors： Gano-Cohen KA,Stokes PJ,Blanton MA,Wendlandt CE,Hollowell AC,Regus JU,Kim D,Patel S,Pahua VJ,Sachs JL

更新日期：2016-08-15 00:00:00

abstract：:A genetic transformation system with similarities to those reported for gram-negative bacteria was found to be associated with membrane vesicles of the ruminal cellulolytic genus Ruminococcus. Double-stranded DNA was recovered from the subcellular particulate fraction of all the cellulolytic ruminococci examined. Elec...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Yokoyama MT,Forster RJ,Ouwerkerk D,Bain PA,Mawhinney EL

更新日期：2005-08-01 00:00:00

abstract：:The effect of the glutathione reductase (GshR) activity of Lactobacillus sanfranciscensis DSM20451(T) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. The gshR gene coding for a putative GshR was sequenced and inactivated by single-crossover integr...

journal_title：Applied and environmental microbiology

authors： Jänsch A,Korakli M,Vogel RF,Gänzle MG

更新日期：2007-07-01 00:00:00

abstract：:Sakacin A is an antilisterial bacteriocin produced by Lactobacillus sake Lb706. In order to identify genes involved in sakacin A production and immunity, the plasmid fraction of L. sake Lb706 was shotgun cloned directly into a sakacin A-nonproducing and -sensitive variant, L. sake Lb706-B, by using the broad-host-rang...

journal_title：Applied and environmental microbiology

authors： Axelsson L,Holck A,Birkeland SE,Aukrust T,Blom H

更新日期：1993-09-01 00:00:00

abstract：:Bacterioplankton numbers, biomasses, and productivities, as well as chlorophyll a concentrations and phytoplankton productivities, were assayed from 1 March 1984 to 12 August 1985 through a 250-m-deep seawater column in Howe Sound, a temperate fjord-sound on the southern coast of British Columbia, Canada. Primary prod...

journal_title：Applied and environmental microbiology

authors： Albright LJ,McCrae SK

更新日期：1987-06-01 00:00:00

abstract：:Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplifie...

journal_title：Applied and environmental microbiology

authors： Gratacap-Cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM

更新日期：2000-06-01 00:00:00