Precipitation of barite by Myxococcus xanthus: possible implications for the biogeochemical cycle of barium.

abstract：:A method was developed for extraction of DNA from Chroococcidiopsis that overcomes obstacles posed by bacterial contamination and the presence of a thick envelope surrounding the cyanobacterial cells. The method is based on the resistance of Chroococcidiopsis to lysozyme and consists of a lysozyme treatment followed b...

journal_title：Applied and environmental microbiology

authors： Billi D,Grilli Caiola M,Paolozzi L,Ghelardini P

更新日期：1998-10-01 00:00:00

abstract：:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it t...

journal_title：Applied and environmental microbiology

authors： Egland PG,Gibson J,Harwood CS

更新日期：2001-03-01 00:00:00

abstract：:The cloned 135-kDa CryIC delta-endotoxin from Bacillus thuringiensis is a lepidopteran-active toxin, displaying high activity in vivo against Spodoptera litoralis and Spodoptera frugiperda larvae and in vitro against the S. frugiperda Sf9 cell line. Here, we report that the CryIC delta-endotoxin cloned from B. thuring...

journal_title：Applied and environmental microbiology

authors： Smith GP,Merrick JD,Bone EJ,Ellar DJ

更新日期：1996-02-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

journal_title：Applied and environmental microbiology

authors： Oh HM,Lee SJ,Jang MH,Yoon BD

更新日期：2000-01-01 00:00:00

abstract：:Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the prese...

journal_title：Applied and environmental microbiology

authors： Brinig MM,Lepp PW,Ouverney CC,Armitage GC,Relman DA

更新日期：2003-03-01 00:00:00

abstract：:Salmonella is a zoonotic pathogen that poses a considerable public health and economic burden in the United States and worldwide. Resultant human diseases range from enterocolitis to bacteremia to sepsis and are acutely dependent on the particular serovar of Salmonella enterica subsp. enterica, which comprises over 99...

journal_title：Applied and environmental microbiology

authors： Patterson AS,Heithoff DM,Ferguson BS,Soh HT,Mahan MJ,Plaxco KW

更新日期：2013-04-01 00:00:00

abstract：:Although the molecular surveillance network RotaNet-Italy provides useful nationwide data on rotaviruses causing severe acute gastroenteritis in children in Italy, scarce information is available on rotavirus circulation in the general Italian population, including adults with mild or asymptomatic infection. We invest...

journal_title：Applied and environmental microbiology

authors： Ruggeri FM,Bonomo P,Ianiro G,Battistone A,Delogu R,Germinario C,Chironna M,Triassi M,Campagnuolo R,Cicala A,Giammanco GM,Castiglia P,Serra C,Gaggioli A,Fiore L

更新日期：2015-01-01 00:00:00

abstract：:The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...

journal_title：Applied and environmental microbiology

authors： Choi JJ,Song JG,Nam KH,Lee JI,Bae H,Kim GA,Sun Y,Kwon ST

更新日期：2008-11-01 00:00:00

abstract：:beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...

journal_title：Applied and environmental microbiology

authors： Araujo A,Ward OP

更新日期：1990-06-01 00:00:00

abstract：:To assess the extent of genotypic and phenotypic diversity within species of purple nonsulfur bacteria found in aquatic sediments, a total of 128 strains were directly isolated from agar plates that had been inoculated with sediment samples from Haren and De Biesbosch in The Netherlands. All isolates were initially ch...

journal_title：Applied and environmental microbiology

authors： Oda Y,Wanders W,Huisman LA,Meijer WG,Gottschal JC,Forney LJ

更新日期：2002-07-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:1,2-Dibromoethane (EDB), which is a groundwater contaminant in areas where it was once used as a soil fumigant, was shown to be degraded aerobically by microorganisms in two types of surface soils from an EDB-contaminated groundwater discharge area. At initial concentrations of 6 to 8 mug/liter, EDB was degraded in a ...

journal_title：Applied and environmental microbiology

authors： Pignatello JJ

更新日期：1986-03-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess t...

journal_title：Applied and environmental microbiology

authors： Toyota-Hanatani Y,Ekawa T,Ohta H,Igimi S,Hara-Kudo Y,Sasai K,Baba E

更新日期：2009-02-01 00:00:00

abstract：:Throughout four field trials, compost heaps covered with finished compost maintained temperatures under the physical covering that were ca. 7 to 15.5°C higher, resulting in rapid Escherichia coli O157:H7 reduction, than those of the heaps covered with fresh straw or left uncovered. Our results validated recommendation...

journal_title：Applied and environmental microbiology

authors： Shepherd MW Jr,Kim J,Jiang X,Doyle MP,Erickson MC

更新日期：2011-07-01 00:00:00

abstract：:In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, wh...

journal_title：Applied and environmental microbiology

authors： Cocolin L,Urso R,Rantsiou K,Cantoni C,Comi G

更新日期：2006-01-01 00:00:00

abstract：:This work reports the utilization of an in vivo expression technology system to identify in vivo-induced (ivi) genes in Yersinia ruckeri after determination of the conditions needed for its selection in fish. Fourteen clones were selected, and the cloned DNA fragments were analyzed after partial sequencing. In additio...

journal_title：Applied and environmental microbiology

authors： Fernández L,Márquez I,Guijarro JA

更新日期：2004-09-01 00:00:00

abstract：:Fusarium isolates from specific diseased sorghum plants and rain-soaked wheat and wheat flour associated with human mycotoxicosis in India have been screened for their toxigenic potential. Of the 322 isolates screened, 11 isolates were found to produce deoxynivalenol in concentrations ranging from 0.01 to 186 microgra...

journal_title：Applied and environmental microbiology

authors： Ramakrishna Y,Bhat RV,Ravindranath V

更新日期：1989-10-01 00:00:00

abstract：:Intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. The quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microor...

journal_title：Applied and environmental microbiology

authors： Ercolini D,De Filippis F,La Storia A,Iacono M

更新日期：2012-11-01 00:00:00

abstract：:Sludges derived from wastewater treatment are foul-smelling, biologically unstable substances. As well as containing numerous pathogenic microorganisms, they also consist of organic matter that can be used as agricultural fertilizer. Legislation nevertheless requires sludges to be virologically tested prior to spreadi...

journal_title：Applied and environmental microbiology

authors： Monpoeho S,Maul A,Bonnin C,Patria L,Ranarijaona S,Billaudel S,Ferré V

更新日期：2004-09-01 00:00:00

abstract：:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

journal_title：Applied and environmental microbiology

authors： Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

更新日期：2007-10-01 00:00:00

abstract：:Cell extracts of Clostridium thermohydrosulfuricum, an anaerobic bacterium which ferments starch into ethanol at 65 degrees C, contained both pullulanase and glucoamylase activities. The general physiochemical and catalytic properties of these enzyme activities were compared. Pullulanase and glucoamylase activities we...

journal_title：Applied and environmental microbiology

authors： Hyun HH,Zeikus JG

更新日期：1985-05-01 00:00:00

abstract：:Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group-specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PB...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA

更新日期：1995-04-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both...

journal_title：Applied and environmental microbiology

authors： Li B,Nagalla SR,Renganathan V

更新日期：1997-02-01 00:00:00

abstract：:We characterized the intracellular symbiotic microbiota of the bamboo pseudococcid Antonina crawii by performing a molecular phylogenetic analysis in combination with in situ hybridization. Almost the entire length of the bacterial 16S rRNA gene was amplified and cloned from A. crawii whole DNA. Restriction fragment l...

journal_title：Applied and environmental microbiology

authors： Fukatsu T,Nikoh N

更新日期：2000-02-01 00:00:00