General Biochemical Characterization of Thermostable Pullulanase and Glucoamylase from Clostridium thermohydrosulfuricum.

abstract：:The gene cluster that codes for feedback-resistant aspartate kinase (lysCalpha and lysCbeta) and aspartate semialdehyde dehydrogenase (asd) was cloned from a mutant strain of Corynebacterium glutamicum. Its functional analysis by subcloning, enzyme assays, and type of aspartate kinase regulation enabled the isolation ...

journal_title：Applied and environmental microbiology

authors： Cremer J,Eggeling L,Sahm H

更新日期：1991-06-01 00:00:00

abstract：:We examined nitrate-dependent Fe(2+) oxidation mediated by anaerobic ammonium oxidation (anammox) bacteria. Enrichment cultures of "Candidatus Brocadia sinica" anaerobically oxidized Fe(2+) and reduced NO3(-) to nitrogen gas at rates of 3.7 ± 0.2 and 1.3 ± 0.1 (mean ± standard deviation [SD]) nmol mg protein(-1) min(-...

journal_title：Applied and environmental microbiology

authors： Oshiki M,Ishii S,Yoshida K,Fujii N,Ishiguro M,Satoh H,Okabe S

更新日期：2013-07-01 00:00:00

abstract：:Essential oil components (EOCs) have limited water solubility and are used at much higher concentrations in complex food matrices than in growth media to inhibit pathogens. However, the correlation between solubility and activity has not been studied. The objective of this work was to characterize the solubility of EO...

journal_title：Applied and environmental microbiology

authors： Chen H,Davidson PM,Zhong Q

更新日期：2014-02-01 00:00:00

abstract：:Pigment production by the fungus Monascus sp. was studied to determine why Monascus sp. provides more pigment in solid culture than in submerged culture. Adding a sterilized nonionic polymeric adsorbent resin directly to the growing submerged culture did not enhance the pigment production, thus indicating that pigment...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Wang HY

更新日期：1984-06-01 00:00:00

abstract：:Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

journal_title：Applied and environmental microbiology

authors： Helton RR,Liu L,Wommack KE

更新日期：2006-07-01 00:00:00

abstract：:Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO2 (n-TiO2) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were i...

journal_title：Applied and environmental microbiology

authors： Zhang P,Guo JS,Yan P,Chen YP,Wang W,Dai YZ,Fang F,Wang GX,Shen Y

更新日期：2018-04-16 00:00:00

abstract：:In the current work, we describe genome diversity and core genome sequences among representatives of three bifidobacterial species, i.e., Bifidobacterium adolescentis, Bifidobacterium catenulatum, and Bifidobacterium pseudocatenulatum, by employing a polyphasic approach involving analysis of 16S rRNA gene and 16S-23S ...

journal_title：Applied and environmental microbiology

authors： Duranti S,Turroni F,Milani C,Foroni E,Bottacini F,Dal Bello F,Ferrarini A,Delledonne M,van Sinderen D,Ventura M

更新日期：2013-01-01 00:00:00

abstract：:Enterococcus faecalis encodes a biotin-dependent oxaloacetate decarboxylase (OAD), which is constituted by four subunits: E. faecalis carboxyltransferase subunit OadA (termed Ef-A), membrane pump Ef-B, biotin acceptor protein Ef-D, and the novel subunit Ef-H. Our results show that in E. faecalis, subunits Ef-A, Ef-D, ...

journal_title：Applied and environmental microbiology

authors： Repizo GD,Blancato VS,Mortera P,Lolkema JS,Magni C

更新日期：2013-05-01 00:00:00

abstract：:Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phosph...

journal_title：Applied and environmental microbiology

authors： MacNaughton SJ,Stephen JR,Venosa AD,Davis GA,Chang YJ,White DC

更新日期：1999-08-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous yeast protease. Thi...

journal_title：Applied and environmental microbiology

authors： Salamin K,Sriranganadane D,Léchenne B,Jousson O,Monod M

更新日期：2010-07-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:Zearalenone [6-(10-hydroxy-6-oxo-trans-1-undecenyl)beta-resorcyclic acid lactone] is a hormone produced by Fusarium spp. which regulates the sexual stage in F. roseum. 3H- and 14C-labeled zearalenone were found to bind preferentially to one of two peaks containing uncharacterized proteins obtained from the cytosol of ...

journal_title：Applied and environmental microbiology

authors： Inaba T,Mirocha CJ

更新日期：1979-01-01 00:00:00

abstract：:The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the...

journal_title：Applied and environmental microbiology

authors： Fischer-Le Saux M,Hervio-Heath D,Loaec S,Colwell RR,Pommepuy M

更新日期：2002-11-01 00:00:00

abstract：:The presence and activities of urease genes were investigated in 49 clinical, food, and environmental Bacillus cereus isolates. Ten strains were shown to have urease genes, with eight of these strains showing growth on urea as the sole nitrogen source. Two of the urease-positive strains, including the sequenced strain...

journal_title：Applied and environmental microbiology

authors： Mols M,Abee T

更新日期：2008-04-01 00:00:00

abstract：:Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...

journal_title：Applied and environmental microbiology

authors： Prol García MJ,D'Alvise PW,Gram L

更新日期：2013-09-01 00:00:00

abstract：:This work reports on the identification and molecular characterization of a two-component regulatory system (2CRS), encoded by serRK, which is believed to control the expression of the ser(2003) locus in Bifidobacterium breve UCC2003. The ser(2003) locus consists of two genes, Bbr_1319 (sagA) and Bbr_1320 (serU), whic...

journal_title：Applied and environmental microbiology

authors： Alvarez-Martin P,O'Connell Motherway M,Turroni F,Foroni E,Ventura M,van Sinderen D

更新日期：2012-10-01 00:00:00

abstract：:Aerobic and anaerobic microorganisms were enumerated in tubercles collected from sections of the water distribution pipeline in the Columbus, Ohio, metropolitan area. Coliform bacteria were not detected in the tubercles examined. Sulfate-reducing bacteria were detected in 80% of the samples. Nitrate-reducing heterotro...

journal_title：Applied and environmental microbiology

authors： Tuovinen OH,Hsu JC

更新日期：1982-09-01 00:00:00

abstract：:Reservoir 29 and Lake B are extremely acid lakes (epilimnion pHs of 2.7 and 3.2, respectively), because they receive acidic discharges from coal refuse piles. They differ in that the pH of profundal sediments in Reservoir 29 increased from 2.7 to 3.8 during the period of thermal stratification, whereas permanently ano...

journal_title：Applied and environmental microbiology

authors： Gyure RA,Konopka A,Brooks A,Doemel W

更新日期：1987-09-01 00:00:00

abstract：:Several probiotic strains of Bifidobacterium animalis subsp. lactis are widely supplemented into food products and dietary supplements due to their documented health benefits and ability to survive within the mammalian gastrointestinal tract and acidified dairy products. The strain specificity of these characteristics...

journal_title：Applied and environmental microbiology

authors： Briczinski EP,Loquasto JR,Barrangou R,Dudley EG,Roberts AM,Roberts RF

更新日期：2009-12-01 00:00:00

abstract：:Sakacin G is a 37-amino-acid-residue-long class IIa bacteriocin produced by Lactobacillus sake 2512, which is encoded by the duplicated structural genes skgA1 and skgA2. Sakacin G appears to be unique and seems to be an intermediate between pediocin-like bacteriocins, according to its double-disulfide bridges required...

journal_title：Applied and environmental microbiology

authors： Simon L,Fremaux C,Cenatiempo Y,Berjeaud JM

更新日期：2002-12-01 00:00:00

abstract：:Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extrarad...

journal_title：Applied and environmental microbiology

authors： Kuremoto K,Noiri Y,Ishimoto T,Yoneda N,Yamamoto R,Maezono H,Nakano T,Hayashi M,Ebisu S

更新日期：2014-07-01 00:00:00

abstract：:Wieners and sausages were prepared which contained the most heat-tolerant representative of the Mycobacterium avium-Mycobacterium intracellulare complex we were able to obtain. They also were prepared with infected tissues obtained from tuberculous swine. Processing conditions were as varied as possible. Neither incor...

journal_title：Applied and environmental microbiology

authors： Merkal RS,Crawford JA,Whipple DL

更新日期：1979-11-01 00:00:00

abstract：:The temperate bacteriophage phiLC3, isolated from Lactococcus lactis subsp. cremoris, has an isometric head and a flexible tail containing 1 major protein and 8 minor proteins. Infection of a permissive L. lactis host strain yields a burst of about 50 phages per infected cell with a latent period of 60 min. A detailed...

journal_title：Applied and environmental microbiology

authors： Lillehaug D,Lindqvist B,Birkeland NK

更新日期：1991-11-01 00:00:00

abstract：:The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of p...

journal_title：Applied and environmental microbiology

authors： Choi JW,Stoecker DK

更新日期：1989-07-01 00:00:00

abstract：:The utilization of the fuel oxygenate methyl tert-butyl ether (MTBE) and related compounds by microorganisms was investigated in a mainly theoretical study based on the Y(ATP) concept. Experiments were conducted to derive realistic maintenance coefficients and K(s) values needed to calculate substrate fluxes available...

journal_title：Applied and environmental microbiology

authors： Müller RH,Rohwerder T,Harms H

更新日期：2007-03-01 00:00:00

abstract：:Alteromonas sp. strain O-7 secretes several proteins in addition to chitinolytic enzymes in response to chitin induction. In this paper, we report that one of these proteins, designated MprIII, is a metalloprotease involved in the chitin degradation system of the strain. The gene encoding MprIII was cloned in Escheric...

journal_title：Applied and environmental microbiology

authors： Miyamoto K,Nukui E,Hirose M,Nagai F,Sato T,Inamori Y,Tsujibo H

更新日期：2002-11-01 00:00:00

abstract：:The influence of physiological and environmental factors on the accumulation of oxindole during anaerobic indole metabolism was investigated by high-performance liquid chromatography. Under methanogenic conditions, indole was temporarily converted to oxindole in stoichiometric amounts in media inoculated with three fr...

journal_title：Applied and environmental microbiology

authors： Madsen EL,Francis AJ,Bollag JM

更新日期：1988-01-01 00:00:00

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

journal_title：Applied and environmental microbiology

authors： Keleti G,Sykora JL

更新日期：1982-01-01 00:00:00

abstract：:An optical method to quantify the fungal hyphae within decomposing leaves of deciduous trees was developed. The plant matrix was partially destroyed under hydrolytic conditions, and fungal hyphae and cellulose residues within the leaves were stained with Calcofluor M2R. Cellulose residues were subsequently depolymeriz...

journal_title：Applied and environmental microbiology

authors： Daniel O,Schonholzer F,Zeyer J

更新日期：1995-11-01 00:00:00