Effects of fixation on cell volume of marine planktonic protozoa.

Abstract:

:The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of physiological state. The volume of fixed cells was found to be approximately 20 to 55% lower than the cell volume of live organisms. For the heterotrophic microflagellates, the fixatives ranked, in order of decreasing effect on cell volume, as glutaraldehyde, formaldehyde, acid Lugol's solution, and modified van der Veer solution. With oligotrichous ciliates and a tintinnid ciliate, formaldehyde caused less shrinkage than glutaraldehyde or acid Lugol's solution. With the aldehyde fixatives, the microflagellates were found to shrink more than the ciliates. Differential effects of fixation on cell volumes may result in an underestimation of the biomass of certain protozoan taxa in natural samples.

journal_name

Appl Environ Microbiol

authors

Choi JW,Stoecker DK

doi

10.1128/AEM.55.7.1761-1765.1989

keywords:

subject

Has Abstract

pub_date

1989-07-01 00:00:00

pages

1761-5

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

55

pub_type

杂志文章
  • Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria.

    abstract::Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.4363-4366.2004

    authors: Schoenborn L,Yates PS,Grinton BE,Hugenholtz P,Janssen PH

    更新日期:2004-07-01 00:00:00

  • Interaction of Aeromonas strains with lactic acid bacteria via Caco-2 cells.

    abstract::The genus Aeromonas includes some species that have now been identified as human pathogens of significant medical importance. We investigated the ability of 13 selected Aeromonas strains belonging to nine species isolated from clinical cases (n = 5), environmental waters (n = 5), and fish (n = 3) to adhere to and tran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03200-13

    authors: Hatje E,Neuman C,Katouli M

    更新日期:2014-01-01 00:00:00

  • Dynamics of the methanogenic archaeal community during plant residue decomposition in an anoxic rice field soil.

    abstract::Incorporation of plant residues strongly enhances the methane production and emission from flooded rice fields. Temperature and residue type are important factors that regulate residue decomposition and CH(4) production. However, the response of the methanogenic archaeal community to these factors in rice field soil i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00070-08

    authors: Peng J,Lü Z,Rui J,Lu Y

    更新日期:2008-05-01 00:00:00

  • Novel Coprinopsis cinerea polyesterase that hydrolyzes cutin and suberin.

    abstract::Three cutinase gene-like genes from the basidiomycete Coprinopsis cinerea (Coprinus cinereus) found with a similarity search were cloned and expressed in Trichoderma reesei under the control of an inducible cbh1 promoter. The selected transformants of all three polyesterase constructs showed activity with p-nitropheny...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02103-08

    authors: Kontkanen H,Westerholm-Parvinen A,Saloheimo M,Bailey M,Rättö M,Mattila I,Mohsina M,Kalkkinen N,Nakari-Setälä T,Buchert J

    更新日期:2009-04-01 00:00:00

  • Sequence variability of P2-like prophage genomes carrying the cytolethal distending toxin V operon in Escherichia coli O157.

    abstract::Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01134-13

    authors: Sváb D,Horváth B,Maróti G,Dobrindt U,Tóth I

    更新日期:2013-08-01 00:00:00

  • Identification of Phytophthora citrophthora with Cloned DNA Probes.

    abstract::Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.3.669-674.1990

    authors: Goodwin PH,Kirkpatrick BC,Duniway JM

    更新日期:1990-03-01 00:00:00

  • Form of Vitamin B(12) and Its Role in a Methanol-Utilizing Bacterium Protaminobacter ruber.

    abstract::A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B(12). The compounds were isolated from the cells and identified as methylcobalamin (methyl-B(12)) and adenosylcobalamin (adenosyl-B(12)) by various tests. The variation in the form of B(12) during cultivation was examined by bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.3.515-521.1977

    authors: Sato K,Ueda S,Shimizu S

    更新日期:1977-03-01 00:00:00

  • Prevalence of enterotoxigenic Escherichia coli in some processed raw food from animal origin.

    abstract::Eighteen of 1,200 colonies of Escherichia coli isolated from "keebe," hamburger, or sausage produced heat-labile enterotoxin. None of them produced heat-stable enterotoxin. The characteristics of 9 of the 18 strains are presented. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.270-271.1980

    authors: Reis MH,Vasconcelos JC,Trabulsi LR

    更新日期:1980-01-01 00:00:00

  • Effect of dissolved oxygen and Eh and Bacteroides fragilis during continuous culture.

    abstract::Bacteroides fragilis subsp. fragilis was maintained in a chemostat modified for anaerobic conditions to test the effects of dissolved oxygen and Eh on growth. Using a defined medium containing glucose and a dilution rate of 0.16 h -1, a stable population of 3 X 10(9) colony-forming units/ml was present. At this steady...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.2.168-172.1976

    authors: Onderdonk AB,Johnston J,Mayhew JW,Gorbach SL

    更新日期:1976-02-01 00:00:00

  • Differential effects of temperature on natural transformation to erythromycin and nalidixic acid resistance in Campylobacter coli.

    abstract::Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01075-08

    authors: Kim JS,Kim JW,Kathariou S

    更新日期:2008-10-01 00:00:00

  • Helix alpha 4 of the Bacillus thuringiensis Cry1Aa toxin plays a critical role in the postbinding steps of pore formation.

    abstract::Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01930-08

    authors: Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

    更新日期:2009-01-01 00:00:00

  • Methods for integrated air sampling and dna analysis for detection of airborne fungal spores.

    abstract::Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2453-2459.2001

    authors: Williams RH,Ward E,McCartney HA

    更新日期:2001-06-01 00:00:00

  • Biosynthesis of Poly-beta-Hydroxyalkanoates from Pentoses by Pseudomonas pseudoflava.

    abstract::The potential of Pseudomonas pseudoflava to produce poly-beta-hydroxyalkanoates (PHAs) from pentoses was studied. This organism was able to use a hydrolysate from the hemicellulosic fraction of poplar wood as a carbon and energy source for its growth. However, in batch cultures, growth was inhibited completely at hydr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.10.3133-3138.1990

    authors: Bertrand JL,Ramsay BA,Ramsay JA,Chavarie C

    更新日期:1990-10-01 00:00:00

  • Engineering of Bacillus subtilis for enhanced total synthesis of folic acid.

    abstract::We investigated whether the yield of the B vitamin folic acid could be elevated in Bacillus subtilis. Strategies for increasing the folic acid yield were investigated by employing computer-aided flux analysis and mutation. Controlling the activity of the enzyme pyruvate kinase by placing it under inducible control was...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.7122-7129.2005

    authors: Zhu T,Pan Z,Domagalski N,Koepsel R,Ataai MM,Domach MM

    更新日期:2005-11-01 00:00:00

  • Modular engineering of L-tyrosine production in Escherichia coli.

    abstract::Efficient biosynthesis of L-tyrosine from glucose is necessary to make biological production economically viable. To this end, we designed and constructed a modular biosynthetic pathway for L-tyrosine production in E. coli MG1655 by encoding the enzymes for converting erythrose-4-phosphate (E4P) and phosphoenolpyruvat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06017-11

    authors: Juminaga D,Baidoo EE,Redding-Johanson AM,Batth TS,Burd H,Mukhopadhyay A,Petzold CJ,Keasling JD

    更新日期:2012-01-01 00:00:00

  • P1 Trisaccharide (Galalpha1,4Galbeta1,4GlcNAc) synthesis by enzyme glycosylation reactions using recombinant Escherichia coli.

    abstract::The frequency of Escherichia coli infection has lead to concerns over pathogenic bacteria in our food supply and a demand for therapeutics. Glycolipids on gut cells serve as receptors for the Shiga-like toxin produced by E. coli. Oligosaccharide moiety analogues of these glycolipids can compete with receptors for the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.4.2110-2115.2003

    authors: Liu Z,Lu Y,Zhang J,Pardee K,Wang PG

    更新日期:2003-04-01 00:00:00

  • Predictive models for Escherichia coli concentrations at inland lake beaches and relationship of model variables to pathogen detection.

    abstract::Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02995-12

    authors: Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

    更新日期:2013-03-01 00:00:00

  • Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification.

    abstract::Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.7933-7940.2005

    authors: Maruyama F,Kenzaka T,Yamaguchi N,Tani K,Nasu M

    更新日期:2005-12-01 00:00:00

  • Inactivation of Giardia muris cysts by free chlorine.

    abstract::The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.7.1448-1453.1987

    authors: Leahy JG,Rubin AJ,Sproul OJ

    更新日期:1987-07-01 00:00:00

  • Construction and Optimization of a Heterologous Pathway for Protocatechuate Catabolism in Escherichia coli Enables Bioconversion of Model Aromatic Compounds.

    abstract::The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01313-17

    authors: Clarkson SM,Giannone RJ,Kridelbaugh DM,Elkins JG,Guss AM,Michener JK

    更新日期:2017-08-31 00:00:00

  • Novel probiotic Bifidobacterium bifidum CECT 7366 strain active against the pathogenic bacterium Helicobacter pylori.

    abstract::Helicobacter pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers. Moreover, 50% of the population carries this bacterium, and consequently, when it is detected, eradication of H. pylori is strongly recommended. Regarding the use of probiotics as fu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01820-10

    authors: Chenoll E,Casinos B,Bataller E,Astals P,Echevarría J,Iglesias JR,Balbarie P,Ramón D,Genovés S

    更新日期:2011-02-01 00:00:00

  • 2-Aminoethylphosphonic acid concentrations in some rumen ciliate protozoa.

    abstract::The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.4.951-953.1983

    authors: Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

    更新日期:1983-10-01 00:00:00

  • Purification, characterization, and gene cloning of a novel maltosyltransferase from an Arthrobacter globiformis strain that produces an alternating alpha-1,4- and alpha-1,6-cyclic tetrasaccharide from starch.

    abstract::A glycosyltransferase, involved in the synthesis of cyclic maltosylmaltose [CMM; cyclo-{-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->6)-alpha-D-Glcp(1-->4)-alpha-D-Glcp(1-->}] from starch, was purified to homogeneity from the culture supernatant of Arthrobacter globiformis M6. The CMM-forming enzyme had a molecular mass...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.2.1065-1071.2006

    authors: Mukai K,Watanabe H,Kubota M,Chaen H,Fukuda S,Kurimoto M

    更新日期:2006-02-01 00:00:00

  • Isolation of Thermus strains from hot composts (60 to 80 degrees C).

    abstract::High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.5.1723-1727.1996

    authors: Beffa T,Blanc M,Lyon PF,Vogt G,Marchiani M,Fischer JL,Aragno M

    更新日期:1996-05-01 00:00:00

  • VqsA, a Novel LysR-Type Transcriptional Regulator, Coordinates Quorum Sensing (QS) and Is Controlled by QS To Regulate Virulence in the Pathogen Vibrio alginolyticus.

    abstract::The quorum sensing (QS) system controls bacterial group behaviors in response to cell density. In vibrios, LuxR and AphA are two master QS regulators (MQSRs) controlling gene expression in response to high or low cell density. Other regulators involved in the regulation of these two MQSRs and QS pathways remain to be ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00444-18

    authors: Gao X,Wang X,Mao Q,Xu R,Zhou X,Ma Y,Liu Q,Zhang Y,Wang Q

    更新日期:2018-05-31 00:00:00

  • Enhanced Growth of Wheat and Soybean Plants Inoculated with Azospirillum brasilense Is Not Necessarily Due to General Enhancement of Mineral Uptake.

    abstract::The capacity of Azospirillum brasilense to enhance the accumulation of K, P, Ca, Mg, S, Na, Mn, Fe, B, Cu, and Zn in inoculated wheat and soybean plants was evaluated by using two different analytical methods with five A. brasilense strains originating from four distinct geographical regions. A Pseudomonas isolate fro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.3.769-775.1990

    authors: Bashan Y,Harrison SK,Whitmoyer RE

    更新日期:1990-03-01 00:00:00

  • Isolation of novel extreme-tolerant cyanobacteria from a rock-dwelling microbial community by using exposure to low Earth orbit.

    abstract::Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02547-09

    authors: Olsson-Francis K,de la Torre R,Cockell CS

    更新日期:2010-04-01 00:00:00

  • Arginine deiminase system and bacterial adaptation to acid environments.

    abstract::The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.1.198-200.1987

    authors: Marquis RE,Bender GR,Murray DR,Wong A

    更新日期:1987-01-01 00:00:00

  • Shedding of Escherichia coli O157:H7 in calves is reduced by prior colonization with the homologous strain.

    abstract::Enterohemorrhagic Escherichia coli O157:H7 has a natural reservoir in the intestinal tracts of cattle. Colonization is asymptomatic and transient, but it is not clear if protective immunity is induced. This study demonstrates that prior colonization induces humoral immune responses to bacterial antigens and reduces ba...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02670-06

    authors: Naylor SW,Flockhart A,Nart P,Smith DG,Huntley J,Gally DL,Low JC

    更新日期:2007-06-01 00:00:00

  • Carbohydrate and Amino Acid Fermentation in the Free-Living Primitive Protozoon Hexamita sp.

    abstract::Hexamita sp. is an amitochondriate free-living diplomonad which inhabits O(2)-limited environments, such as the deep waters and sediments of lakes and marine basins. C nuclear magnetic resonance spectroscopy reveals ethanol, lactate, acetate, and alanine as products of glucose fermentation under microaerobic condition...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.1.203-207.1998

    authors: Biagini GA,McIntyre PS,Finlay BJ,Lloyd D

    更新日期:1998-01-01 00:00:00