Abstract:
:The effects of fixation on the cell volume of marine heterotrophic nanoflagellates and planktonic ciliates were investigated. Decreases in cell volume depended on the combination of the protozoan taxa and the particular fixative. For a particular fixative and protozoan species, degree of shrinkage was independent of physiological state. The volume of fixed cells was found to be approximately 20 to 55% lower than the cell volume of live organisms. For the heterotrophic microflagellates, the fixatives ranked, in order of decreasing effect on cell volume, as glutaraldehyde, formaldehyde, acid Lugol's solution, and modified van der Veer solution. With oligotrichous ciliates and a tintinnid ciliate, formaldehyde caused less shrinkage than glutaraldehyde or acid Lugol's solution. With the aldehyde fixatives, the microflagellates were found to shrink more than the ciliates. Differential effects of fixation on cell volumes may result in an underestimation of the biomass of certain protozoan taxa in natural samples.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Choi JW,Stoecker DKdoi
10.1128/AEM.55.7.1761-1765.1989keywords:
subject
Has Abstractpub_date
1989-07-01 00:00:00pages
1761-5issue
7eissn
0099-2240issn
1098-5336journal_volume
55pub_type
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