Form of Vitamin B(12) and Its Role in a Methanol-Utilizing Bacterium Protaminobacter ruber.

Abstract:

:A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B(12). The compounds were isolated from the cells and identified as methylcobalamin (methyl-B(12)) and adenosylcobalamin (adenosyl-B(12)) by various tests. The variation in the form of B(12) during cultivation was examined by bioautography with cellulose acetate membrane electrophoresis. Methyl-B(12) and adenosyl-B(12) were the two main B(12) compounds produced in the various phases of bacterial growth. The ratio of the amount of methyl-B(12) to total B(12) compounds was higher during the earlier phases of growth. After the logarithmic phase, adenosyl-B(12) was the predominant form. The existence of N-methyltetrahydrofolate:homocysteine transmethylase and methyl-B(12):homocysteine transmethylase was demonstrated in cell-free extracts of Protaminobacter ruber. Methyl-B(12) in P. ruber seems to function mainly in the B(12)-dependent methionine synthetase system.

journal_name

Appl Environ Microbiol

authors

Sato K,Ueda S,Shimizu S

doi

10.1128/AEM.33.3.515-521.1977

keywords:

subject

Has Abstract

pub_date

1977-03-01 00:00:00

pages

515-21

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

33

pub_type

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