Proteolytic activity of the ruminal bacterium Butyrivibrio fibrisolvens.

abstract：:Salmonella is a zoonotic pathogen that poses a considerable public health and economic burden in the United States and worldwide. Resultant human diseases range from enterocolitis to bacteremia to sepsis and are acutely dependent on the particular serovar of Salmonella enterica subsp. enterica, which comprises over 99...

journal_title：Applied and environmental microbiology

authors： Patterson AS,Heithoff DM,Ferguson BS,Soh HT,Mahan MJ,Plaxco KW

更新日期：2013-04-01 00:00:00

abstract：:The fungus Cladosporium sphaerospermum was isolated from a biofilter used for the removal of toluene from waste gases. This is the first report describing growth of a eukaryotic organism with toluene as the sole source of carbon and energy. The oxygen consumption rates, as well as the measured enzyme activities, of to...

journal_title：Applied and environmental microbiology

authors： Weber FJ,Hage KC,de Bont JA

更新日期：1995-10-01 00:00:00

abstract：:A technique to determine which nutrients limit bacterial growth in soil was developed. The method was based on measuring the thymidine incorporation rate of bacteria after the addition of C, N, and P in different combinations to soil samples. First, the thymidine incorporation method was tested in two different soils:...

journal_title：Applied and environmental microbiology

authors： Aldén L,Demoling F,Bååth E

更新日期：2001-04-01 00:00:00

abstract：:Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region aff...

journal_title：Applied and environmental microbiology

authors： Yang H,Shi P,Liu Y,Xia W,Wang X,Cao H,Ma R,Luo H,Bai Y,Yao B

更新日期：2017-03-02 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:Mycobiota growing on food is often beneficial for the ripening and development of the specific flavor characteristics of the product, but it can also be harmful due to the production of undesirable compounds such as mycotoxins or antibiotics. Some of the fungi most frequently isolated from fermented and cured meat pro...

journal_title：Applied and environmental microbiology

authors： Laich F,Fierro F,Martín JF

更新日期：2002-03-01 00:00:00

abstract：:In a previous study by our group, CH4 oxidation and N2 fixation were simultaneously activated in the roots of wild-type rice plants in a paddy field with no N input; both processes are likely controlled by a rice gene for microbial symbiosis. The present study examined which microorganisms in rice roots were responsib...

journal_title：Applied and environmental microbiology

authors： Bao Z,Okubo T,Kubota K,Kasahara Y,Tsurumaru H,Anda M,Ikeda S,Minamisawa K

更新日期：2014-08-01 00:00:00

abstract：:Streptococcus mutans is a key cariogenic bacterium responsible for the initiation of tooth decay. Biofilm formation is a crucial virulence property. We discovered a putative glycosyltransferase, SMU_833, in S. mutans capable of modulating dynamic interactions between two key biofilm matrix components, glucan and extra...

journal_title：Applied and environmental microbiology

authors： Rainey K,Michalek SM,Wen ZT,Wu H

更新日期：2019-02-20 00:00:00

abstract：:Lactic and acetic acids were determined to be slightly synergistic in their inhibitory interrelationship against Salmonella typhimurium with the use of a modified toxicological model. ...

journal_title：Applied and environmental microbiology

authors： Rubin HE

更新日期：1978-10-01 00:00:00

abstract：:Two methods were used to evaluate bacterial recovery from beef, pork, and lamb adipose tissue. Higher counts were obtained with a tissue removal and fluid agitation technique (shaking) than with surface swabbing, but only when bacterial levels were low. Bacterial recovery by both methods was unaffected by specie origi...

journal_title：Applied and environmental microbiology

authors： Berry BW,Joseph AL,Chen AA

更新日期：1978-05-01 00:00:00

abstract：:Enterotoxin B produced by Staphylococus aureus 243 in brain heart infusion broth was concentrated by dialysis against 40% polyethylene glycol (20 M), partially purified on a Sephadex G-100 column and heated at 110 degrees C in thermal death time cans. Various heating menstrua included 0.04 M Veronal buffer (pH 7.4), b...

journal_title：Applied and environmental microbiology

authors： Lee IC,Stevenson KE,Harmon LG

更新日期：1977-02-01 00:00:00

abstract：:Of more than 400 bacteria isolated from turbot (Scophthalmus maximus), 89 have previously been shown to inhibit the in vitro growth of the fish pathogen Vibrio anguillarum. The aim of the present study was to investigate the potential of seven of these strains, as well as of intestinal isolates (four strains) from a c...

journal_title：Applied and environmental microbiology

authors： Olsson JC,Westerdahl A,Conway PL,Kjelleberg S

更新日期：1992-02-01 00:00:00

abstract：:Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M Na...

journal_title：Applied and environmental microbiology

authors： Korber DR,Choi A,Wolfaardt GM,Caldwell DE

更新日期：1996-11-01 00:00:00

abstract：:Taxonomic and phylogenetic fingerprinting based on sequence analysis of gene fragments from the large-subunit rRNA (LSU) gene or the internal transcribed spacer (ITS) region is becoming an integral part of fungal classification. The lack of an accurate and robust classification tool trained by a validated sequence dat...

journal_title：Applied and environmental microbiology

authors： Liu KL,Porras-Alfaro A,Kuske CR,Eichorst SA,Xie G

更新日期：2012-03-01 00:00:00

abstract：:The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

journal_title：Applied and environmental microbiology

authors： Desomer J,Dhaese P,Montagu MV

更新日期：1990-09-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:Although molecular techniques have identified Helicobacter pylori in drinking water-associated biofilms, there is a lack of studies reporting what factors affect the attachment of the bacterium to plumbing materials. Therefore, the adhesion of H. pylori suspended in distilled water to stainless steel 304 (SS304) coupo...

journal_title：Applied and environmental microbiology

authors： Azevedo NF,Pinto AR,Reis NM,Vieira MJ,Keevil CW

更新日期：2006-04-01 00:00:00

abstract：:The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. A total of 545 genes were flagged on the basis of signific...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Gaskell J,Mozuch M,Kersten P,Sabat G,Martinez D,Cullen D

更新日期：2009-06-01 00:00:00

abstract：:DNA primers corresponding to conserved motifs in bacterial repetitive (REP, ERIC, and BOX) elements and PCR were used to show that REP-, ERIC-, and BOX-like DNA sequences are widely distributed in phytopathogenic Xanthomonas and Pseudomonas strains. REP-, ERIC, and BOX-PCR (collectively known as rep-PCR) were used to ...

journal_title：Applied and environmental microbiology

authors： Louws FJ,Fulbright DW,Stephens CT,de Bruijn FJ

更新日期：1994-07-01 00:00:00

abstract：:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation mi...

journal_title：Applied and environmental microbiology

authors： Rizzi A,Leroux J,Charron-Lamoureux V,Roy S,Beauregard PB,Bellenger JP

更新日期：2020-10-28 00:00:00

abstract：:Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

journal_title：Applied and environmental microbiology

authors： Liu W,Hansen JN

更新日期：1990-08-01 00:00:00

abstract：:Differences in individual host responses have emerged as an issue regarding the health benefits of probiotics. Here, we applied ribosome engineering (RE) technology, developed in an actinomycete study, to Lacticaseibacillus rhamnosus GG (LGG). RE can effectively enhance microbial potential by using antibiotics to indu...

journal_title：Applied and environmental microbiology

authors： Ishida M,Namai F,Shigemori S,Kajikawa S,Tsukagoshi M,Sato T,Ogita T,Shimosato T

更新日期：2020-10-01 00:00:00

abstract：:Between November 2002 and April 2003, 244 bottles and cartons of commercially pasteurized cow's milk were obtained at random from retail outlets throughout the Czech Republic. During the same period, samples of raw milk and of milk that was subsequently subjected to a minimum of 71.7 degrees C for 15 s in a local past...

journal_title：Applied and environmental microbiology

authors： Ayele WY,Svastova P,Roubal P,Bartos M,Pavlik I

更新日期：2005-03-01 00:00:00

abstract：:Most associations between animals and their gut microbiota are dynamic, involving sustained transfer of food-associated microbial cells into the gut and shedding of microorganisms into the external environment with feces, but the interacting effects of host and microbial factors on the composition of the internal and ...

journal_title：Applied and environmental microbiology

authors： Wong AC,Luo Y,Jing X,Franzenburg S,Bost A,Douglas AE

更新日期：2015-09-01 00:00:00

abstract：:Five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of Bacillus thuringiensis subsp. israelensis. Bacterial powders prepared from the broth cultures were assayed against the larvae of Aedes aegypti, C...

journal_title：Applied and environmental microbiology

authors： Obeta JA,Okafor N

更新日期：1984-04-01 00:00:00

abstract：:We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. col...

journal_title：Applied and environmental microbiology

authors： Salinger N,Kokona B,Fairman R,Okeke IN

更新日期：2009-01-01 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:Most denitrifiers produce nitrous oxide (N(2)O) instead of dinitrogen (N(2)) under aerobic conditions. We isolated and characterized novel aerobic denitrifiers that produce low levels of N(2)O under aerobic conditions. We monitored the denitrification activities of two of the isolates, strains TR2 and K50, in batch an...

journal_title：Applied and environmental microbiology

authors： Takaya N,Catalan-Sakairi MA,Sakaguchi Y,Kato I,Zhou Z,Shoun H

更新日期：2003-06-01 00:00:00

abstract：:The pathway of electrons required for the reduction of sulfate in sulfate-reducing bacteria (SRB) is not yet fully characterized. In order to determine the role of a transmembrane protein complex suggested to be involved in this process, a deletion in Desulfovibrio vulgaris Hildenborough was created by marker exchange...

journal_title：Applied and environmental microbiology

authors： Zane GM,Yen HC,Wall JD

更新日期：2010-08-01 00:00:00

abstract：:Gordonia polyisoprenivorans strain VH2, a potent rubber-degrading actinomycete, harbors two latex clearing proteins (Lcps), which are known to be essential for the microbial degradation of rubber. However, biochemical information on the exact role of this protein in the degradation of polyisoprene was lacking. In this...

journal_title：Applied and environmental microbiology

authors： Hiessl S,Böse D,Oetermann S,Eggers J,Pietruszka J,Steinbüchel A

更新日期：2014-09-01 00:00:00