Abstract:
:We demonstrate that enhanced lysozyme resistance of enteropathogenic Escherichia coli requires the plasmid-encoded regulator, Per, and is mediated by factors outside the locus for enterocyte effacement. EspC, a Per-activated serine protease autotransporter protein, conferred enhanced resistance on nonpathogenic E. coli, and a second Per-regulated, espC-independent lysozyme resistance mechanism was identified.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Salinger N,Kokona B,Fairman R,Okeke INdoi
10.1128/AEM.01734-08subject
Has Abstractpub_date
2009-01-01 00:00:00pages
275-80issue
1eissn
0099-2240issn
1098-5336pii
AEM.01734-08journal_volume
75pub_type
杂志文章abstract::Sixty-three strains of lactic streptococci isolated from commercial lactic streptococcal starter cultures were examined for lysogeny by treatment with ultraviolet light or mitomycin C. After treatment with the inducing agent, all strains, whether or not they lysed, were examined for evidence of phage release by electr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.1.184-191.1977
更新日期:1977-01-01 00:00:00
abstract::The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01688-12
更新日期:2012-10-01 00:00:00
abstract::Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.62-68.1988
更新日期:1988-01-01 00:00:00
abstract::The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1379-1384.1985
更新日期:1985-06-01 00:00:00
abstract::The bacterial epiphyte Pseudomonas syringae MF714R was cultured on agar or in broth or collected from colonized leaves; it was then inoculated onto greenhouse-grown bean plants incubated in a growth chamber at low relative humidity or in the field or onto field-grown bean plants. Cells cultured in liquid medium surviv...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.2.410-416.1993
更新日期:1993-02-01 00:00:00
abstract::The mechanisms of metal resistance of a cadmium-resistant Alcaligenes sp. were studied. Growth in a defined medium was unaffected by cadmium at concentrations up to 0.1 mM, while at concentrations up to 2.5 mM, growth occurred after an extended lag phase. The increase in length of the lag phase was abolished by repeat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.3.515-520.1986
更新日期:1986-03-01 00:00:00
abstract::The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the leading vector-borne illness in the United States. Many of the genetic factors affecting spirochete morphology and physiology are unknown due to the limited genetic tools available and the large number of open reading frames with unknown f...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.5973-5979.2004
更新日期:2004-10-01 00:00:00
abstract::A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph Methylomonas methanica on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.4.960-967.1993
更新日期:1993-04-01 00:00:00
abstract::Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00038-06
更新日期:2006-07-01 00:00:00
abstract::Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.3.1268-1273.2001
更新日期:2001-03-01 00:00:00
abstract::Environmental studies of the human-pathogenic bacterium Campylobacter jejuni have focused on linking distributions with potential sources. However, in aquatic ecosystems, the abundance of C. jejuni may also be regulated by predation. We examine the potential for grazing by the freshwater planktonic crustacean Daphnia ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.9.5085-5088.2005
更新日期:2005-09-01 00:00:00
abstract::Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02238-07
更新日期:2008-03-01 00:00:00
abstract::Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.3.1473-1479.2005
更新日期:2005-03-01 00:00:00
abstract::A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.5.3217-3227.2006
更新日期:2006-05-01 00:00:00
abstract::Inactivation of infectious viruses during drinking water treatment is usually achieved with free chlorine. Many drinking water utilities in the United States now use monochloramine as a secondary disinfectant to minimize disinfectant by-product formation and biofilm growth. The inactivation of human adenoviruses 2, 40...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01342-09
更新日期:2010-02-01 00:00:00
abstract::Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00853-12
更新日期:2012-07-01 00:00:00
abstract::Acidophilic archaea of the archaeal Richmond Mine acidophilic nanoorganisms (ARMAN) group from the uncultured candidate phylum "Candidatus Micrarchaeota" have small genomes and cell sizes and are known to be metabolically dependent and physically associated with their Thermoplasmatales hosts. However, phylogenetically...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02199-20
更新日期:2020-11-10 00:00:00
abstract::Identifying and overcoming the limitations preventing efficient high-yield production of chemicals remain important tasks in metabolic engineering. In an attempt to rewire Corynebacterium glutamicum to produce ethanol, we attained a low yield (63% of the theoretical) when using resting cells on glucose, and large amou...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02114-20
更新日期:2020-11-24 00:00:00
abstract::Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.7.1638-1644.1987
更新日期:1987-07-01 00:00:00
abstract:UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04042-15
更新日期:2016-04-18 00:00:00
abstract::Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03867-14
更新日期:2015-04-01 00:00:00
abstract::Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03723-14
更新日期:2015-03-01 00:00:00
abstract::Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable le...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02487-08
更新日期:2009-06-01 00:00:00
abstract::Environmental surveillance is an effective approach in investigating circulating enteroviruses and had been conducted in the cities of Jinan and Linyi since February 2008 and April 2010, respectively. This study analyzed 46 sewage samples collected in the two cities in 2011 and found that echovirus 6 (E6) was the pred...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01861-12
更新日期:2012-10-01 00:00:00
abstract::We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.3.683-687.1988
更新日期:1988-03-01 00:00:00
abstract::Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of eac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02780-09
更新日期:2010-08-01 00:00:00
abstract::Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01908-20
更新日期:2021-01-04 00:00:00
abstract::Two strains of 1,1,1-trichloroethane (TCA)-degrading bacteria, TA5 and TA27, were isolated from soil and identified as Mycobacterium spp. Strains TA5 and TA27 could degrade 25 and 75 mg. liter of TCA(-1) cometabolically in the presence of ethane as a carbon source, respectively. The compound 2,2,2-trichloroethanol was...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.10.4693-4696.1999
更新日期:1999-10-01 00:00:00
abstract::So far, only scarce and rather diffuse information is available on bacteriophages infecting members of the genus Bifidobacterium. In the current study, we investigated the genetic organization, phylogenetic relationships, and, in some cases, transcription profiles and inducibility of 19 prophage-like elements present ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01112-09
更新日期:2009-11-01 00:00:00
abstract::The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-d-arab...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.3.781-785.1992
更新日期:1992-03-01 00:00:00