Occurrence and molecular characteristics of methicillin-resistant Staphylococcus aureus and methicillin-resistant Staphylococcus pseudintermedius in an academic veterinary hospital.

Abstract:

:Recently, methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) have been increasingly isolated from veterinarians and companion animals. With a view to preventing the spread of MRSA and MRSP, we evaluated the occurrence and molecular characteristics of each in a veterinary college. MRSA and MRSP were isolated from nasal samples from veterinarians, staff members, and veterinary students affiliated with a veterinary hospital. Using stepwise logistic regression, we identified two factors associated with MRSA carriage: (i) contact with an identified animal MRSA case (odds ratio [OR], 6.9; 95% confidence interval [95% CI], 2.2 to 21.6) and (ii) being an employee (OR, 6.2; 95% CI, 2.0 to 19.4). The majority of MRSA isolates obtained from individuals affiliated with the veterinary hospital and dog patients harbored spa type t002 and a type II staphylococcal cassette chromosome mec (SCCmec), similar to the hospital-acquired MRSA isolates in Japan. MRSA isolates harboring spa type t008 and a type IV SCCmec were obtained from one veterinarian on three different sampling occasions and also from dog patients. MRSA carriers can also be a source of MRSA infection in animals. The majority of MRSP isolates (85.2%) carried hybrid SCCmec type II-III, and almost all the remaining MRSP isolates (11.1%) carried SCCmec type V. MRSA and MRSP were also isolated from environmental samples collected from the veterinary hospital (5.1% and 6.4%, respectively). The application of certain disinfection procedures is important for the prevention of nosocomial infection, and MRSA and MRSP infection control strategies should be adopted in veterinary medical practice.

journal_name

Appl Environ Microbiol

authors

Ishihara K,Shimokubo N,Sakagami A,Ueno H,Muramatsu Y,Kadosawa T,Yanagisawa C,Hanaki H,Nakajima C,Suzuki Y,Tamura Y

doi

10.1128/AEM.02780-09

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

5165-74

issue

15

eissn

0099-2240

issn

1098-5336

pii

AEM.02780-09

journal_volume

76

pub_type

杂志文章
  • Depth-related changes in community structure of culturable mineral weathering bacteria and in weathering patterns caused by them along two contrasting soil profiles.

    abstract::Bacteria play important roles in mineral weathering and soil formation. However, few reports of mineral weathering bacteria inhabiting subsurfaces of soil profiles have been published, raising the question of whether the subsurface weathering bacteria are fundamentally distinct from those in surface communities. To ad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02335-13

    authors: Huang J,Sheng XF,Xi J,He LY,Huang Z,Wang Q,Zhang ZD

    更新日期:2014-01-01 00:00:00

  • Formation of stable bdelloplasts as a starvation-survival strategy of marine bdellovibrios.

    abstract::Several wild-type isolates of marine bdellovibrios formed stable bdelloplasts when they infected gram-negative bacterial prey under certain culture conditions. Synchronous predator-prey cultures and low nutrient concentrations increased the yield of stable bdelloplasts. The bdellovibrio cells retained in the stable bd...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2717-2725.1990

    authors: Sánchez-Amat A,Torrella F

    更新日期:1990-09-01 00:00:00

  • Rapid extraction of plasmids from Clostridium perfringens.

    abstract::Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.521-523.1986

    authors: Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

    更新日期:1986-03-01 00:00:00

  • Effects of Sulfate Reduction on Trichloroethene Dechlorination by Dehalococcoides-Containing Microbial Communities.

    abstract::In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03384-16

    authors: Mao X,Polasko A,Alvarez-Cohen L

    更新日期:2017-03-31 00:00:00

  • Novel Sampling Method for Assessing Human-Pathogen Interactions in the Natural Environment Using Boot Socks and Citizen Scientists, with Application to Campylobacter Seasonality.

    abstract::This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00162-17

    authors: Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

    更新日期:2017-06-30 00:00:00

  • The N-Acetylglucosaminidase LytB of Streptococcus pneumoniae Is Involved in the Structure and Formation of Biofilms.

    abstract::The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00280-20

    authors: Domenech M,García E

    更新日期:2020-05-05 00:00:00

  • Mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, MnP2.

    abstract::Unlike general peroxidases, Pleurotus ostreatus MnP2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as Poly R-478 and RNase A. To elucidate the mechanism for oxidation of polymeric substrates by MnP2, a series of mutant enzymes were produced by using a homologous ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02080-07

    authors: Tsukihara T,Honda Y,Sakai R,Watanabe T,Watanabe T

    更新日期:2008-05-01 00:00:00

  • Diversity and seasonal variability of beta-Proteobacteria in biofilms of polluted rivers: analysis by temperature gradient gel electrophoresis and cloning.

    abstract::The beta-subgroup of the Proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16S rRNA techniques in biofilms of the Elbe River and its polluted tributary, the Spittelwasser River. The bacterial 16S rRNA genes were cloned from each site, screened for beta-pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.8.4463-4473.2003

    authors: Brümmer IH,Felske A,Wagner-Döbler I

    更新日期:2003-08-01 00:00:00

  • Induction and Characterization of Artificial Diploids from the Haploid Yeast Torulaspora delbrueckii.

    abstract::The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.7.1504-1511.1987

    authors: Sasaki T,Ohshima Y

    更新日期:1987-07-01 00:00:00

  • Species-specific cell wall binding affinity of the S-layer proteins of mosquitocidal bacterium Bacillus sphaericus C3-41.

    abstract::The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00356-09

    authors: Li J,Hu X,Yan J,Yuan Z

    更新日期:2009-06-01 00:00:00

  • Characterization of the rRNA locus of Pfiesteria piscicida and development of standard and quantitative PCR-based detection assays targeted to the nontranscribed spacer.

    abstract::Pfiesteria piscicida is a heterotrophic dinoflagellate widely distributed along the middle Atlantic shore of the United States and associated with fish kills in the Neuse River (North Carolina) and the Chesapeake Bay (Maryland and Virginia). We constructed a genomic DNA library from clonally cultured P. piscicida and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.11.5394-5407.2002

    authors: Saito K,Drgon T,Robledo JA,Krupatkina DN,Vasta GR

    更新日期:2002-11-01 00:00:00

  • Ethylene Production by Pseudomonas syringae Pathovars In Vitro and In Planta.

    abstract::Significant amounts of ethylene were produced by Pseudomonas syringae pv. glycinea, pv. phaseolicola (which had been isolated from viny weed Pueraria lobata [Willd.] Ohwi [common name, kudzu]), and pv. pisi in synthetic medium. On the other hand, the bean strains of P. syringae pv. phaseolicola and strains of 17 other...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.1.156-161.1997

    authors: Weingart H,Volksch B

    更新日期:1997-01-01 00:00:00

  • Effect of bacterial distribution and activity on conjugal gene transfer on the phylloplane of the bush bean (Phaseolus vulgaris).

    abstract::Conjugal plasmid transfer was examined on the phylloplane of bean (Phaseolus vulgaris) and related to the spatial distribution pattern and metabolic activity of the bacteria. The donor (Pseudomonas putida KT2442) harbored a derivative of the TOL plasmid, which conferred kanamycin resistance and had the gfp gene insert...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.5.1902-1909.1998

    authors: Normander B,Christensen BB,Molin S,Kroer N

    更新日期:1998-05-01 00:00:00

  • Production and secretion in Escherichia coli of hepatitis B virus pre-S2 antigen as fusion proteins with beta-lactamase.

    abstract::The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2742-2747.1990

    authors: Kadokura H,Yoda K,Imai M,Yamasaki M

    更新日期:1990-09-01 00:00:00

  • Vector Competence of Geographical Populations of Ornithodoros turicata for the Tick-Borne Relapsing Fever Spirochete Borrelia turicatae.

    abstract::Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01505-18

    authors: Krishnavajhala A,Armstrong BA,Lopez JE

    更新日期:2018-10-17 00:00:00

  • Bioconversion of alpha-Damascone by Botrytis cinerea.

    abstract::Bioconversion of alpha-damascone (compound 1) was studied with four strains of Botrytis cinerea in grape must (pH 3.2). As biotransformation products of compound 1, 3-oxo-alpha-damascone, cis- and trans-3-hydroxy-alpha-damascone, gamma-damascenone, 3-oxo-8, 9-dihydro-alpha-damascone, and cis- and trans-3-hydroxy-8,9-d...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.1.15-18.1991

    authors: Schoch E,Benda I,Schreier P

    更新日期:1991-01-01 00:00:00

  • Monitoring microbial populations and antibiotic resistance gene enrichment associated with Arctic waste stabilization ponds.

    abstract::Wastewater management in the Canadian Arctic is challenging due to climate extremes, small population sizes, and lack of conventional infrastructure for wastewater treatment. Although many Northern communities use waste stabilization ponds (WSPs) as their primary form of wastewater treatment, few studies have explored...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02914-20

    authors: Gromala M,Neufeld JD,McConkey BJ

    更新日期:2021-01-15 00:00:00

  • The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway.

    abstract::Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01401-08

    authors: Kolkman MA,van der Ploeg R,Bertels M,van Dijk M,van der Laan J,van Dijl JM,Ferrari E

    更新日期:2008-12-01 00:00:00

  • Microorganisms associated with uranium bioremediation in a high-salinity subsurface sediment.

    abstract::Although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been shown to successfully remove uranium from some aquifer sediments, the organisms in the family Geobacteraceae that have been found to be associated with metal reduction in previous studies are not known to g...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3672-3675.2003

    authors: Nevin KP,Finneran KT,Lovley DR

    更新日期:2003-06-01 00:00:00

  • Metabolic engineering of Acinetobacter baylyi ADP1 for improved growth on gluconate and glucose.

    abstract::A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01837-14

    authors: Kannisto M,Aho T,Karp M,Santala V

    更新日期:2014-11-01 00:00:00

  • High prevalence of vancomycin-resistant enterococci in Swedish sewage.

    abstract::In Europe the use of the growth promoter avoparcin is considered to have selected for vancomycin-resistant enterococci (VRE). Sweden ceased using avoparcin in 1986, and only occasional cases of VRE from hospitals have been reported since 1995. Within the framework of a European study, samples from urban raw sewage, tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.6.2838-2842.2002

    authors: Iversen A,Kühn I,Franklin A,Möllby R

    更新日期:2002-06-01 00:00:00

  • Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.

    abstract::Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.493-497.1986

    authors: Desjardins AE,Plattner RD,Vanmiddlesworth F

    更新日期:1986-03-01 00:00:00

  • Molecular basis of the behavior of hepatitis a virus exposed to high hydrostatic pressure.

    abstract::Food-borne hepatitis A outbreaks may be prevented by subjecting foods at risk of virus contamination to moderate treatments of high hydrostatic pressure (HHP). A pretreatment promoting hepatitis A virus (HAV) capsid-folding changes enhances the virucidal effect of HHP, indicating that its efficacy depends on capsid co...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01693-14

    authors: D'Andrea L,Pérez-Rodríguez FJ,Costafreda MI,Beguiristain N,Fuentes C,Aymerich T,Guix S,Bosch A,Pintó RM

    更新日期:2014-10-01 00:00:00

  • Role of the bga1-encoded extracellular {beta}-galactosidase of Hypocrea jecorina in cellulase induction by lactose.

    abstract::Lactose is the only soluble and economically feasible carbon source for the production of cellulases or heterologous proteins regulated by cellulase expression signals by Hypocrea jecorina (Trichoderma reesei). We investigated the role of the major beta-galactosidase of H. jecorina in lactose metabolism and cellulase ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.2.851-857.2005

    authors: Seiboth B,Hartl L,Salovuori N,Lanthaler K,Robson GD,Vehmaanperä J,Penttilä ME,Kubicek CP

    更新日期:2005-02-01 00:00:00

  • Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

    abstract::A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4432-4439.2004

    authors: Chen H,Ponniah G,Salonen N,Blum P

    更新日期:2004-08-01 00:00:00

  • Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat.

    abstract::The production of microcystins (MC) from Microcystis aeruginosa UTEX 2388 was investigated in a P-limited continuous culture. MC (MC-LR, MC-RR, and MC-YR) from lyophilized M. aeruginosa were extracted with 5% acetic acid, purified by a Sep-Pak C(18) cartridge, and then analyzed by high-performance liquid chromatograph...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.1.176-179.2000

    authors: Oh HM,Lee SJ,Jang MH,Yoon BD

    更新日期:2000-01-01 00:00:00

  • Effect of dietary aflatoxin on fertility, hatchability, and progeny performance of broiler breeder hens.

    abstract::The effects of aflatoxin on egg production, fertility, hatchability, and progeny performance were investigated by feeding dietary aflatoxin at dose levels of 0,5, and 10 mug/g to mature broiler breeder hens for 4 weeks. Sixteen hens were used for each dietary dose level. Egg production decreased significantly during w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.5.680-684.1976

    authors: Howarth B Jr,Wyatt RD

    更新日期:1976-05-01 00:00:00

  • Use of root organ cultures to investigate the interaction between Glomus intraradices and Pratylenchus coffeae.

    abstract::The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.4308-4311.2003

    authors: Elsen A,Declerck S,De Waele D

    更新日期:2003-07-01 00:00:00

  • Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp.

    abstract::A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.11.3346-3353.1990

    authors: Lauff JJ,Steele DB,Coogan LA,Breitfeller JM

    更新日期:1990-11-01 00:00:00

  • Soil Fungicides for Controlling Chytridiaceous Mycoparasites of Gigaspora margarita and Glomus fasciculatum.

    abstract::The percentage of azygospores of Gigaspora margarita with zoosporangia of chytridiaceous fungi (CF) was reduced significantly after agitating them in fenaminosulf before incubation in soil. Fenaminosulf did not affect zoosporangia development on chlamydospores of Glomus fasciculatum. Metalaxyl and ethazol were not eff...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.4.1306-1309.1983

    authors: Sylvia DM,Schenck NC

    更新日期:1983-04-01 00:00:00