The N-Acetylglucosaminidase LytB of Streptococcus pneumoniae Is Involved in the Structure and Formation of Biofilms.

abstract：:The rumen anaerobic fungus Neocallimastix frontalis was grown on cellulosic substrates, and the cellular distribution and types of glycosidases produced by the organism were studied. Fungal cultures were fractionated into extracellular, insoluble (membrane), and intracellular fractions and assayed for glycosidase acti...

journal_title：Applied and environmental microbiology

authors： Pearce PD,Bauchop T

更新日期：1985-05-01 00:00:00

abstract：:Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...

journal_title：Applied and environmental microbiology

authors： Geng T,Morgan MT,Bhunia AK

更新日期：2004-10-01 00:00:00

abstract：:Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage sequences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the g...

journal_title：Applied and environmental microbiology

authors： Ventura M,Canchaya C,Bernini V,Altermann E,Barrangou R,McGrath S,Claesson MJ,Li Y,Leahy S,Walker CD,Zink R,Neviani E,Steele J,Broadbent J,Klaenhammer TR,Fitzgerald GF,O'toole PW,van Sinderen D

更新日期：2006-05-01 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

journal_title：Applied and environmental microbiology

authors： Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

更新日期：1989-08-01 00:00:00

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...

journal_title：Applied and environmental microbiology

authors： Vishnivetskaya TA,Kathariou S

更新日期：2005-11-01 00:00:00

abstract：:Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by int...

journal_title：Applied and environmental microbiology

authors： Tao F,Yu B,Xu P,Ma CQ

更新日期：2006-07-01 00:00:00

abstract：:Corynebacterium glutamicum is able to grow in media containing up to 12 mM arsenite and 500 mM arsenate and is one of the most arsenic-resistant microorganisms described to date. Two operons (ars1 and ars2) involved in arsenate and arsenite resistance have been identified in the complete genome sequence of Corynebacte...

journal_title：Applied and environmental microbiology

authors： Ordóñez E,Letek M,Valbuena N,Gil JA,Mateos LM

更新日期：2005-10-01 00:00:00

abstract：:Bacteriophages are very abundant in the biosphere, and viral infection is believed to affect the activity and genetic diversity of bacterial communities in aquatic environments. Lysogenic conversion, for example, can improve host fitness and lead to phage-mediated horizontal gene transfer. However, little is known abo...

journal_title：Applied and environmental microbiology

authors： Ghosh D,Roy K,Williamson KE,White DC,Wommack KE,Sublette KL,Radosevich M

更新日期：2008-01-01 00:00:00

abstract：:Pure and mixed cultures of Methylophaga sulfidovorans and Thiobacillus thioparus T5 were grown in continuous cultures on either dimethyl sulfide, dimethyl sulfide and H(inf2)S, or H(inf2)S and methanol. In pure cultures, M. sulfidovorans showed a lower affinity for sulfide than T. thioparus T5. Mixed cultures, grown o...

journal_title：Applied and environmental microbiology

authors： De Zwart J,Sluis J,Kuenen JG

更新日期：1997-08-01 00:00:00

abstract：:The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained from oceanic and estuarine seawater and compared with the frequency of QQ enzyme sequences in the available marine metagenomic collections. The possible role of the high QQ activity found among marine bacteria is discussed. ...

journal_title：Applied and environmental microbiology

authors： Romero M,Martin-Cuadrado AB,Otero A

更新日期：2012-09-01 00:00:00

abstract：:The pathogenicity of Listeria monocytogenes as influenced by growth on crabmeat at 5 and 10 degrees C was studied. Crabmeat was inoculated with L. monocytogenes V7 (ca. 10(4) CFU/g) and incubated for up to 14 days at 5 and 10 degrees C. At selected incubation times, L. monocytogenes was removed from crabmeat by washin...

journal_title：Applied and environmental microbiology

authors： Brackett RE,Beuchat LR

更新日期：1990-05-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

journal_title：Applied and environmental microbiology

authors： Mermelstein LD,Papoutsakis ET

更新日期：1993-04-01 00:00:00

abstract：:Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

journal_title：Applied and environmental microbiology

authors： Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

更新日期：2011-03-01 00:00:00

abstract：:Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

journal_title：Applied and environmental microbiology

authors： Szabó I,Benedek A,Barabás G

更新日期：1985-08-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

journal_title：Applied and environmental microbiology

authors： Keleti G,Sykora JL

更新日期：1982-01-01 00:00:00

abstract：:Nonselected and natural populations of Escherichia coli from 12 animal sources and humans were examined for the presence and types of 14 tetracycline resistance determinants. Of 1,263 unique E. coli isolates from humans, pigs, chickens, turkeys, sheep, cows, goats, cats, dogs, horses, geese, ducks, and deer, 31% were ...

journal_title：Applied and environmental microbiology

authors： Bryan A,Shapir N,Sadowsky MJ

更新日期：2004-04-01 00:00:00

abstract：:The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

journal_title：Applied and environmental microbiology

authors： Stahl DA,Lane DJ,Olsen GJ,Pace NR

更新日期：1985-06-01 00:00:00

abstract：:Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg ...

journal_title：Applied and environmental microbiology

authors： Peltola J,Ritieni A,Mikkola R,Grigoriev PA,Pócsfalvi G,Andersson MA,Salkinoja-Salonen MS

更新日期：2004-08-01 00:00:00

abstract：UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...

journal_title：Applied and environmental microbiology

authors： Wang S,Doona CJ,Setlow P,Li YQ

更新日期：2016-09-16 00:00:00

abstract：:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

journal_title：Applied and environmental microbiology

authors： Sembries S,Crawford RL

更新日期：1997-05-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:Enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. Psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperature...

journal_title：Applied and environmental microbiology

authors： Trimbur DE,Gutshall KR,Prema P,Brenchley JE

更新日期：1994-12-01 00:00:00

abstract：:Growth on 3-chlorobenzoate was found to induce the enzymes of the protocatechuate meta ring fission pathway in Alcaligenes sp. strain BR60. The chlorobenzoate catabolic genes, designated cba, were localized to a 3.7-kb NotI-EcoRI fragment within the nonrepeated region of the composite transposon Tn5271. The cba genes ...

journal_title：Applied and environmental microbiology

authors： Nakatsu CH,Wyndham RC

更新日期：1993-11-01 00:00:00

abstract：:A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and red...

journal_title：Applied and environmental microbiology

authors： Thimm T,Tebbe CC

更新日期：2003-05-01 00:00:00

abstract：:Anaerobic ammonium oxidation is a recent addition to the microbial nitrogen cycle, and its metabolic pathway, including the production and conversion of its intermediate hydrazine, is not well understood. Therefore, the effect of hydroxylamine addition on the hydrazine metabolism of anaerobic ammonium-oxidizing (anamm...

journal_title：Applied and environmental microbiology

authors： van der Star WR,van de Graaf MJ,Kartal B,Picioreanu C,Jetten MS,van Loosdrecht MC

更新日期：2008-07-01 00:00:00

abstract：:Penicillium decumbens is able to epoxidize cis-propenylphosphonic acid (cPA) to produce the antibiotic fosfomycin [FOM; also referred to as phosphonomycin and (-)-cis-1,2-epoxypropylphosphonic acid], a bioconversion of considerable commercial significance. We sought to improve the efficiency of the process by overexpr...

journal_title：Applied and environmental microbiology

authors： Watanabe M,Sumida N,Murakami S,Anzai H,Thompson CJ,Tateno Y,Murakami T

更新日期：1999-03-01 00:00:00