Rapid extraction of plasmids from Clostridium perfringens.

abstract：:Currently only five (SEA-SEE) out of 27 known staphylococcal enterotoxins can be analyzed using commercially available kits.Six genes (seg, sei, sem, sen, seo, and seu), encoding putative and undetectable enterotoxins, are located on the enterotoxin gene cluster (egc) which is part of the Staphylococcus aureus genomic...

journal_title：Applied and environmental microbiology

authors： Schwendimann L,Merda D,Berger T,Denayer S,Feraudet Tarisse C,Kläui AJ,Messio S,Mistou MY,Nia Y,Hennekinne JA,Graber HU

更新日期：2020-12-18 00:00:00

abstract：:Emulsan is a polyanionic heteropolysaccharide bioemulsifier produced by Acinetobacter calcoaceticus RAG-1. A mixed bacterial population was obtained by enrichment culture that was capable of degrading emulsan and using it as a carbon source. From this mixed culture, an emulsan-degrading bacterium, termed YUV-1, was is...

journal_title：Applied and environmental microbiology

authors： Shoham Y,Rosenberg M,Rosenberg E

更新日期：1983-09-01 00:00:00

abstract：:Resistance to HIV infection in a cohort of commercial sex workers living in Nairobi, Kenya, is linked to mucosal and antiinflammatory factors that may be influenced by the vaginal microbiota. Since bacterial vaginosis (BV), a polymicrobial dysbiosis characterized by low levels of protective Lactobacillus organisms, is...

journal_title：Applied and environmental microbiology

authors： Schellenberg JJ,Links MG,Hill JE,Dumonceaux TJ,Kimani J,Jaoko W,Wachihi C,Mungai JN,Peters GA,Tyler S,Graham M,Severini A,Fowke KR,Ball TB,Plummer FA

更新日期：2011-06-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

journal_title：Applied and environmental microbiology

authors： Vannelli T,Hooper AB

更新日期：1992-07-01 00:00:00

abstract：:A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceed...

journal_title：Applied and environmental microbiology

authors： Lauff JJ,Steele DB,Coogan LA,Breitfeller JM

更新日期：1990-11-01 00:00:00

abstract：:A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

journal_title：Applied and environmental microbiology

authors： Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

更新日期：2017-08-01 00:00:00

abstract：:Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...

journal_title：Applied and environmental microbiology

authors： Johnson LJ,Miller AN,McCleery RA,McClanahan R,Kath JA,Lueschow S,Porras-Alfaro A

更新日期：2013-09-01 00:00:00

abstract：:The yeast Torulaspora delbrueckii, which propagates as a haploid, was made into a diploid by treatment with dimethyl sulfoxide (DMSO) on the regeneration of protoplasts. The diploid state was stably inherited; the cell volume was three times that of the parent strain and the cellular DNA content was two times that of ...

journal_title：Applied and environmental microbiology

authors： Sasaki T,Ohshima Y

更新日期：1987-07-01 00:00:00

abstract：:The distribution and levels of Clostridium botulinum type E were determined from field sites used by Inuit hunters for butchering seals along the coast of Nunavik. The incidence rates of C. botulinum type E in shoreline soil along the coast were 0, 50, and 87.5% among samples tested for the Hudson Strait, Hudson Bay, ...

journal_title：Applied and environmental microbiology

authors： Leclair D,Farber JM,Doidge B,Blanchfield B,Suppa S,Pagotto F,Austin JW

更新日期：2013-01-01 00:00:00

abstract：:Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. T...

journal_title：Applied and environmental microbiology

authors： Ainsworth TD,Fine M,Blackall LL,Hoegh-Guldberg O

更新日期：2006-04-01 00:00:00

abstract：:Strains of Ascochyta rabiei which are pathogenic to chickpea (Cicer arietinum L.) readily catabolized the main chickpea isoflavone biochanin A (5,7-dihydroxy-4'-methoxyisoflavone). 3'-Hydroxylation and O-demethylation reactions led to the isoflavones pratensein, genistein, and orobol, which were rapidly further degrad...

journal_title：Applied and environmental microbiology

authors： Kraft B,Barz W

更新日期：1985-07-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne pathogen with the ability to grow under conditions of high osmolarity. In a previous study, we reported the identification of 12 proteins showing high induction after salt stress. One of these proteins is highly similar to the general stress protein Ctc of Bacillus subtilis. In t...

journal_title：Applied and environmental microbiology

authors： Gardan R,Duché O,Leroy-Sétrin S,Labadie J,European Listeria Genome Consortium.

更新日期：2003-01-01 00:00:00

abstract：:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

journal_title：Applied and environmental microbiology

authors： Russell EG

更新日期：1979-02-01 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

journal_title：Applied and environmental microbiology

authors： Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

更新日期：2007-02-01 00:00:00

abstract：:A defined solid and liquid minimal medium, HTM, which contained methionine and cysteine as the sole amino acids, was developed for Listeria monocytogenes. Complex broth-grown L. monocytogenes had to adapt to HTM by inducing amino acid biosyntheis. HTM is the simplest minimal medium available for growth of L. monocytog...

journal_title：Applied and environmental microbiology

authors： Tsai HN,Hodgson DA

更新日期：2003-11-01 00:00:00

abstract：:Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

journal_title：Applied and environmental microbiology

authors： Haines JR,Covert TC,Rankin CC

更新日期：1993-08-01 00:00:00

abstract：:A competitive enzyme-linked immunosorbent assay was used to screen for T-2 toxin in Fusarium sporotrichioides -infected corn. The assay detected T-2 toxin in diluted methanol extracts of corn samples at concentrations of 0.05 ng/ml. In infected corn samples, enzyme-linked immunosorbent assay and gas-liquid chromatogra...

journal_title：Applied and environmental microbiology

authors： Gendloff EH,Pestka JJ,Swanson SP,Hart LP

更新日期：1984-05-01 00:00:00

abstract：:The diversity of rhizobia that form symbioses with roots of black locust (Robinia pseudoacacia L.), an economically important leguminous tree species, was examined by inoculating seedling root zones with samples of soil collected from the United States, Canada, and China. Bacteria were isolated from nodules, subcultur...

journal_title：Applied and environmental microbiology

authors： McCray Batzli J,Graves WR,van Berkum P

更新日期：1992-07-01 00:00:00

abstract：:A study of six child care centers was conducted to assess the microbiological quality of three food contact surfaces (one food serving surface and two food preparation surfaces) and one non-food contact surface (diaper changing surface) to determine the effectiveness of cleaning and sanitization procedures within the ...

journal_title：Applied and environmental microbiology

authors： Cosby CM,Costello CA,Morris WC,Haughton B,Devereaux MJ,Harte F,Davidson PM

更新日期：2008-11-01 00:00:00

abstract：:Influenza virus has been found to persist in the environment for hours to days, allowing for secondary transmission of influenza via inanimate objects known as fomites. We evaluated the efficacy of heat and moisture for the decontamination of surfaces for the purpose of preventing of the spread of influenza. Aqueous s...

journal_title：Applied and environmental microbiology

authors： McDevitt J,Rudnick S,First M,Spengler J

更新日期：2010-06-01 00:00:00

abstract：:The structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type I propagation by using singly nine strains of Lactobacillus sanfranciscensis. Under back-slopping propagation with wheat flour type 0 F114, cell numbers of presumptive lactic acid bacteria varie...

journal_title：Applied and environmental microbiology

authors： Siragusa S,Di Cagno R,Ercolini D,Minervini F,Gobbetti M,De Angelis M

更新日期：2009-02-01 00:00:00

abstract：:The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

journal_title：Applied and environmental microbiology

authors： van Niel EW,Gottschal JC

更新日期：1998-03-01 00:00:00

abstract：:We examined the harmful side effects on indigenous soil microorganisms of two organic solvents, acetone and dichloromethane, that are normally used for spiking of soil with polycyclic aromatic hydrocarbons for experimental purposes. The solvents were applied in two contamination protocols to either the whole soil samp...

journal_title：Applied and environmental microbiology

authors： Brinch UC,Ekelund F,Jacobsen CS

更新日期：2002-04-01 00:00:00

abstract：:Scanning and transmission electron microscopy were used to examine the adherence and in situ morphology of the microbial community colonizing the anal and vulvar pores of the subfamily Cyathostominae (Nematoda: Strongylidae) from the colon of Burchell's zebra (Equus burchelli antiquorum). Two different morphological t...

journal_title：Applied and environmental microbiology

authors： Mackie RI,Krecek RC,Els HJ,van Niekerk JP,Kirschner LM,Baecker AA

更新日期：1989-05-01 00:00:00

abstract：:To examine the relationship between activated-sludge bulking and levels of specific filamentous bacteria, we developed a statistics-based quantification method for estimating the biomass levels of specific filaments using 16S rRNA-targeted fluorescent in situ hybridization (FISH) probes. The results of quantitative FI...

journal_title：Applied and environmental microbiology

authors： Liao J,Lou I,de los Reyes FL 3rd

更新日期：2004-04-01 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Cooper DA,Speece R,Henson M

更新日期：1991-06-01 00:00:00

abstract：:A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydrox...

journal_title：Applied and environmental microbiology

authors： Huang K,Rudolph FB,Bennett GN

更新日期：1999-07-01 00:00:00