High prevalence of vancomycin-resistant enterococci in Swedish sewage.

abstract：:In the past 10 years, the number of endosymbionts described within the bacterial order Rickettsiales has constantly grown. Since 2006, 18 novel Rickettsiales genera inhabiting protists, such as ciliates and amoebae, have been described. In this work, we characterize two novel bacterial endosymbionts from Paramecium co...

journal_title：Applied and environmental microbiology

authors： Szokoli F,Castelli M,Sabaneyeva E,Schrallhammer M,Krenek S,Doak TG,Berendonk TU,Petroni G

更新日期：2016-11-21 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00

abstract：:Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination o...

journal_title：Applied and environmental microbiology

authors： Langendijk PS,Schut F,Jansen GJ,Raangs GC,Kamphuis GR,Wilkinson MH,Welling GW

更新日期：1995-08-01 00:00:00

abstract：:A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain transparent bands co...

journal_title：Applied and environmental microbiology

authors： Royer JC,Nakas JP

更新日期：1990-06-01 00:00:00

abstract：:To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

journal_title：Applied and environmental microbiology

authors： Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

更新日期：2010-01-01 00:00:00

abstract：:Demineralization reduced heat resistance of B. subtilis spores, but the pattern and magnitude of the reduction depended on sporulation temperature and on heating menstruum pH. The differences in heat resistance of native spores caused by sporulation temperature almost disappeared after demineralization. Demineralized ...

journal_title：Applied and environmental microbiology

authors： Palop A,Sala FJ,Condón S

更新日期：1999-03-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the United States. Though multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus strains have been reported, resistance patterns in vibrios ...

journal_title：Applied and environmental microbiology

authors： Elmahdi S,Parveen S,Ossai S,DaSilva LV,Jahncke M,Bowers J,Jacobs J

更新日期：2018-01-17 00:00:00

abstract：:The bacterial epiphyte Pseudomonas syringae MF714R was cultured on agar or in broth or collected from colonized leaves; it was then inoculated onto greenhouse-grown bean plants incubated in a growth chamber at low relative humidity or in the field or onto field-grown bean plants. Cells cultured in liquid medium surviv...

journal_title：Applied and environmental microbiology

authors： Wilson M,Lindow SE

更新日期：1993-02-01 00:00:00

abstract：:The accumulation and fate of model microbial "pathogens" within a drinking-water distribution system was investigated in naturally grown biofilms formed in a novel pilot-scale water distribution system provided with chlorinated and UV-treated water. Biofilms were exposed to 1-mum hydrophilic and hydrophobic microspher...

journal_title：Applied and environmental microbiology

authors： Långmark J,Storey MV,Ashbolt NJ,Stenström TA

更新日期：2005-02-01 00:00:00

abstract：:A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or s...

journal_title：Applied and environmental microbiology

authors： Wegmüller B,Lüthy J,Candrian U

更新日期：1993-07-01 00:00:00

abstract：:A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted a...

journal_title：Applied and environmental microbiology

authors： Goto M,Ekino K,Furukawa K

更新日期：1997-07-01 00:00:00

abstract：:A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the...

journal_title：Applied and environmental microbiology

authors： Rosenberg M

更新日期：1981-08-01 00:00:00

abstract：:The products synthesized from a hybrid polyketide synthase/nonribosomal peptide synthetase gene cluster in the genome of Pseudomonas fluorescens Pf-5 were identified using a genomics-guided strategy involving insertional mutagenesis and subsequent metabolite profiling. Five analogs of rhizoxin, a 16-member macrolide w...

journal_title：Applied and environmental microbiology

authors： Loper JE,Henkels MD,Shaffer BT,Valeriote FA,Gross H

更新日期：2008-05-01 00:00:00

abstract：:Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open rea...

journal_title：Applied and environmental microbiology

authors： Ishikawa K,Ishida H,Matsui I,Kawarabayasi Y,Kikuchi H

更新日期：2001-02-01 00:00:00

abstract：:Environmental bacteria persist in various habitats, yet little is known about the genes that contribute to growth and survival in their respective ecological niches. Signature-tagged mutagenesis (STM) of Shewanella oneidensis MR-1 coupled with a screen involving incubations of mutant strains in anoxic aquifer sediment...

journal_title：Applied and environmental microbiology

authors： Groh JL,Luo Q,Ballard JD,Krumholz LR

更新日期：2007-01-01 00:00:00

abstract：:The functioning of recirculation aquaculture systems (RAS) is essential to maintain water quality for fish health, and one crucial process here is nitrification. The investigated RAS was connected to a rainbow trout production system and operated at an average temperature of 13°C and pH 6.8. Community analyses of the ...

journal_title：Applied and environmental microbiology

authors： Hüpeden J,Wegen S,Off S,Lücker S,Bedarf Y,Daims H,Kühn C,Spieck E

更新日期：2016-01-08 00:00:00

abstract：:This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compare...

journal_title：Applied and environmental microbiology

authors： Maheux AF,Boudreau DK,Bisson MA,Dion-Dupont V,Bouchard S,Nkuranga M,Bergeron MG,Rodriguez MJ

更新日期：2014-07-01 00:00:00

abstract：:One of the first steps in characterizing an ecosystem is to describe the organisms inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict diverse communities. Here we describe oligonucleotide fingerprinting of rRNA genes (OFRG), a method that permits identification of arraye...

journal_title：Applied and environmental microbiology

authors： Valinsky L,Della Vedova G,Scupham AJ,Alvey S,Figueroa A,Yin B,Hartin RJ,Chrobak M,Crowley DE,Jiang T,Borneman J

更新日期：2002-07-01 00:00:00

abstract：:Laboratory studies of methane formation in peat samples from an acid subarctic mire in Sweden indicated the presence of a low-temperature-adapted methanogenic flora. Enrichment culture studies with ethanol, acetate, hydrogen, or a combination of these as substrate for methane formation provided evidence for the existe...

journal_title：Applied and environmental microbiology

authors： Svensson BH

更新日期：1984-08-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00

abstract：:A study of six child care centers was conducted to assess the microbiological quality of three food contact surfaces (one food serving surface and two food preparation surfaces) and one non-food contact surface (diaper changing surface) to determine the effectiveness of cleaning and sanitization procedures within the ...

journal_title：Applied and environmental microbiology

authors： Cosby CM,Costello CA,Morris WC,Haughton B,Devereaux MJ,Harte F,Davidson PM

更新日期：2008-11-01 00:00:00

abstract：:This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

journal_title：Applied and environmental microbiology

authors： Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

更新日期：2004-12-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:Prophages make up 12% of the enterohemorrhagic Escherichia coli genome and play prominent roles in the evolution and virulence of this food-borne pathogen. Acquisition and loss of and rearrangements within prophage regions are the primary causes of differences in pulsed-field gel electrophoresis (PFGE) patterns among ...

journal_title：Applied and environmental microbiology

authors： Chen C,Lewis CR,Goswami K,Roberts EL,DebRoy C,Dudley EG

更新日期：2013-03-01 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:Based on results from eight experiments, no overall difference was found between roll tube and three- and five-tube most-probable-number (MPN) methods for estimating total numbers of ruminal bacteria. However, standard errors for the replicate means within an experiment were higher with the MPN procedures. Visual grow...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA,Grifo AP Jr

更新日期：1989-11-01 00:00:00

abstract：:Escherichia coli O157:H7 carried on plant surfaces, including alfalfa sprouts, has been implicated in food poisoning and outbreaks of disease in the United States. Adhesion to cell surfaces is a key component for bacterial establishment and colonization on many types of surfaces. Several E. coli O157:H7 surface protei...

journal_title：Applied and environmental microbiology

authors： Torres AG,Jeter C,Langley W,Matthysse AG

更新日期：2005-12-01 00:00:00

abstract：:The practical task of adapting an original potentiometric technique to the bacteriological analysis of water is discussed. Various laboratory strains of organisms belonging to the usual aquatic flora were inoculated one by one in a minimal lactose broth supplied with lipoic (thioctic) acid. The time evolution of the r...

journal_title：Applied and environmental microbiology

authors： Charriere G,Jouenne T,Lemeland JF,Selegny E,Junter GA

更新日期：1984-01-01 00:00:00

abstract：:Denitrification is an alternative type of anaerobic respiration in which nitrate is reduced to gaseous products via nitrite. The key step in this process is the reduction of nitrite to nitric oxide, which is catalyzed by two structurally different but functionally equivalent forms of nitrite reductase encoded by the n...

journal_title：Applied and environmental microbiology

authors： Pratscher J,Stichternoth C,Fichtl K,Schleifer KH,Braker G

更新日期：2009-02-01 00:00:00

abstract：:Bacillus cereus UW85 produces the linear aminopolyol antibiotic zwittermicin A (ZmA). This antibiotic has diverse biological activities, such as suppression of disease in plants caused by protists, inhibition of fungal and bacterial growth, and amplification of the insecticidal activity of the toxin protein from Bacil...

journal_title：Applied and environmental microbiology

authors： Kevany BM,Rasko DA,Thomas MG

更新日期：2009-02-01 00:00:00