Identification and characterization of spontaneous deletions within the Sp11-Sp12 prophage region of Escherichia coli O157:H7 Sakai.

abstract：:The aim of this work was to investigate the medium requirements for growth and production of exopolysaccharides by Lactobacillus delbrueckii subsp. bulgaricus NCFB 2772. The strain was grown in batch cultures on a chemically defined medium, and the technique of single omission of medium components was applied to deter...

journal_title：Applied and environmental microbiology

authors： Grobben GJ,Chin-Joe I,Kitzen VA,Boels IC,Boer F,Sikkema J,Smith MR,de Bont JA

更新日期：1998-04-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:The soybean is an exotic plant introduced in Paraguay in this century; commercial cropping expanded after the 1970s. Inoculation is practiced in just 15 to 20% of the cropping areas, but root nodulation occurs in most sites where soybeans grow. Little is known about rhizobial diversity in South America, and no study h...

journal_title：Applied and environmental microbiology

authors： Chen LS,Figueredo A,Pedrosa FO,Hungria M

更新日期：2000-11-01 00:00:00

abstract：:The addition of 0.6 M sucrose of 0.016 M Mg2+ to the enumeration medium was required for early expression of heat resistance (10 min at 70 degrees C) in stage V Bacillus cereus forspores. The addition of Mg2+ to the sporulation medium did not remove this requirment for sucrose of Mg2+. The heat damage did not affect f...

journal_title：Applied and environmental microbiology

authors： Busta FF,Baillie E,Murrell WG

更新日期：1976-08-01 00:00:00

abstract：:The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...

journal_title：Applied and environmental microbiology

authors： Dybwad M,Granum PE,Bruheim P,Blatny JM

更新日期：2012-03-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:Pectin is a complex uronic acid-containing polysaccharide typically found in plant cell walls, though forms of pectin are also found in marine diatoms and seagrasses. Genetic loci that target pectin have recently been identified in two phyla of marine bacteria. These loci appear to encode a pectin saccharification pat...

journal_title：Applied and environmental microbiology

authors： Hobbs JK,Hettle AG,Vickers C,Boraston AB

更新日期：2018-12-13 00:00:00

abstract：:The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

journal_title：Applied and environmental microbiology

authors： Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

更新日期：2009-09-01 00:00:00

abstract：:Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

journal_title：Applied and environmental microbiology

authors： Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

更新日期：2010-12-01 00:00:00

abstract：:The bacteriocin nisin is produced only by some strains of Lactococcus lactis, and to date production in other lactic acid bacteria has not been achieved. Enterococcus sp. strain N12beta is a nisin-immune transconjugant obtained from a nisin-producing donor (L. lactis ATCC 11454) and a dairy recipient (Enterococcus sp....

journal_title：Applied and environmental microbiology

authors： Li H,O'Sullivan DJ

更新日期：2002-07-01 00:00:00

abstract：:This is the first study to characterize the environmental conditions which contribute to the presence and proliferation of environmental mycobacteria in a major freshwater river. Over 20 different species of environmental mycobacteria were isolated, including the pathogenic M. avium and M. kansasii. Species of the rap...

journal_title：Applied and environmental microbiology

authors： Bland CS,Ireland JM,Lozano E,Alvarez ME,Primm TP

更新日期：2005-10-01 00:00:00

abstract：:Listeria monocytogenes is among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence of L. monocytogenes strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat a...

journal_title：Applied and environmental microbiology

authors： Balandyté L,Brodard I,Frey J,Oevermann A,Abril C

更新日期：2011-12-01 00:00:00

abstract：:To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U.S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin ge...

journal_title：Applied and environmental microbiology

authors： Xia X,Meng J,McDermott PF,Ayers S,Blickenstaff K,Tran TT,Abbott J,Zheng J,Zhao S

更新日期：2010-03-01 00:00:00

abstract：:Vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. Marine Vibrio spp. produce an array of extracellular products as potential pathogenicity factors. Culture supernatants of V. tubiashii have been shown to be toxic to oyster larvae and were report...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Lind EJ,Boin MA,Häse CC

更新日期：2008-07-01 00:00:00

abstract：:A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...

journal_title：Applied and environmental microbiology

authors： Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GC

更新日期：1996-08-01 00:00:00

abstract：:The biosynthesis of the major carotenoid spirilloxanthin by the purple nonsulfur bacterium Rhodospirillum rubrum is thought to occur via a linear pathway proceeding through phytoene and, later, lycopene as intermediates. This assumption is based solely on early chemical evidence (B. H. Davies, Biochem. J. 116:93-99, 1...

journal_title：Applied and environmental microbiology

authors： Wang GS,Grammel H,Abou-Aisha K,Sägesser R,Ghosh R

更新日期：2012-10-01 00:00:00

abstract：:The respiratory and photosynthetic quinones of microbial mats which occurred in Japanese sulfide-containing neutral-pH hot springs at different temperatures were analyzed by spectrochromatography and mass spectrometry. All of the microbial mats that developed at high temperatures (temperatures above 68 degreesC) were ...

journal_title：Applied and environmental microbiology

authors： Hiraishi A,Umezawa T,Yamamoto H,Kato K,Maki Y

更新日期：1999-01-01 00:00:00

abstract：:We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional ...

journal_title：Applied and environmental microbiology

authors： Liu BH,Chu FS

更新日期：1998-10-01 00:00:00

abstract：:A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

journal_title：Applied and environmental microbiology

authors： Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

更新日期：1997-03-01 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00

abstract：:To prevent transmission of mycobacterial pathogens, medical devices must be disinfected by germicides with proven mycobactericidal activity. The quantitative carrier test EN 14563 provides an international standard for evaluation of the mycobactericidal activity of disinfectants under practical conditions. However, te...

journal_title：Applied and environmental microbiology

authors： Steinhauer K,Eschenbacher I,Radischat N,Detsch C,Niederweis M,Goroncy-Bermes P

更新日期：2010-01-01 00:00:00

abstract：:Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

journal_title：Applied and environmental microbiology

authors： Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

更新日期：1990-08-01 00:00:00

abstract：:Anaerobic methyl tert-butyl ether (MTBE) degradation potential was investigated in samples from a range of sources. From these 22 experimental variations, only one source (from wastewater treatment plant samples) exhibited MTBE degradation. These microcosms were methanogenic and were subjected to DNA-based stable isot...

journal_title：Applied and environmental microbiology

authors： Sun W,Sun X,Cupples AM

更新日期：2012-04-01 00:00:00

abstract：:Lipophilic compounds of the culture suspension containing Rhodococcus erythropolis DSM43215 had surfactant properties when the bacteria were cultivated with n-alkanes as the sole carbon source. Thirteen main components from a dichloromethane-methanol extract of the R. erythropolis cultures were isolated and characteri...

journal_title：Applied and environmental microbiology

authors： Kretschmer A,Bock H,Wagner F

更新日期：1982-10-01 00:00:00

abstract：:Electrorotation is a noninvasive technique that is capable of detecting changes in the morphology and physicochemical properties of microorganisms. Electrorotation studies are reported for two intestinal parasites, Giardia intestinalis and Cyclospora cayetanensis. It is concluded that viable and nonviable G. intestina...

journal_title：Applied and environmental microbiology

authors： Dalton C,Goater AD,Pethig R,Smith HV

更新日期：2001-02-01 00:00:00

abstract：:2-Bromoethanesulfonic acid (BESA) and 2-chloroethanesulfonic acid (CESA) have been reported to be potent inhibitors of methane formation during methanogenic decomposition in batch cultures. However, in a laboratory-scale continuous-flow methanogenic fixed-film column containing a predominance of acetate-decarboxylatin...

journal_title：Applied and environmental microbiology

authors： Bouwer EJ,McCarty PL

更新日期：1983-04-01 00:00:00

abstract：:Superoxide dismutase (SOD) activity was determined during the growth cycle of unheated and heat-injured cells of Staphylococcus aureus MF-31. SOd activity levels dropped in unheated cells during the lag phase, increased during logarithmic phase, and became constant in the stationary phase. Cells which were sublethally...

journal_title：Applied and environmental microbiology

authors： Bucker ER,Martin SE

更新日期：1981-03-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain,...

journal_title：Applied and environmental microbiology

authors： Jimenez IY,Bartha R

更新日期：1996-07-01 00:00:00

abstract：:Several members of the glycoside hydrolase 61 (GH61) family of proteins have recently been shown to dramatically increase the breakdown of lignocellulosic biomass by microbial hydrolytic cellulases. However, purified GH61 proteins have neither demonstrable direct hydrolase activity on various polysaccharide or lignaci...

journal_title：Applied and environmental microbiology

authors： Langston JA,Shaghasi T,Abbate E,Xu F,Vlasenko E,Sweeney MD

更新日期：2011-10-01 00:00:00