Abstract:
:A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very much less active than nisin as an inhibitor of the outgrowth of spores of Bacillus subtilis. These observations, which parallel those of W. Liu and J. N. Hansen (Appl. Environ. Microbiol. 59:648-651, 1993) on the corresponding mutant of the related antibiotic subtilin, are discussed in terms of the mechanism(s) of action of these antibiotics.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Chan WC,Dodd HM,Horn N,Maclean K,Lian LY,Bycroft BW,Gasson MJ,Roberts GCdoi
10.1128/AEM.62.8.2966-2969.1996subject
Has Abstractpub_date
1996-08-01 00:00:00pages
2966-9issue
8eissn
0099-2240issn
1098-5336journal_volume
62pub_type
杂志文章abstract::Physical agents, such as low electric voltage and current, have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric current was shown to suppress the growth of bacteria, it remains unclear to what extent the microampere current damaged the bacterial...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01015-20
更新日期:2020-08-03 00:00:00
abstract::Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03803-14
更新日期:2015-05-01 00:00:00
abstract::A novel strain of Halomonas (Tc-202), which has the capability of removing Tc(VII) from solid- and aqueous-phase material aerobically, was isolated from the marine environment. Tc-202 removed 55% of the total 99Tc in solutions at 15 degrees C by reducing Tc(VII) to Tc(V), but other Halomonas strains did not. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00819-06
更新日期:2006-12-01 00:00:00
abstract::Subsurface sediments were recovered from a 52-m-deep borehole cored in the 300 Area of the Hanford Site in southeastern Washington State to assess the potential for biogeochemical transformation of radionuclide contaminants. Microbial analyses were made on 17 sediment samples traversing multiple geological units: the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.07404-11
更新日期:2012-02-01 00:00:00
abstract::The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01415-06
更新日期:2007-01-01 00:00:00
abstract::Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.9.3196-3202.1996
更新日期:1996-09-01 00:00:00
abstract::A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.1.224-228.1989
更新日期:1989-01-01 00:00:00
abstract::A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.4.967-971.1981
更新日期:1981-04-01 00:00:00
abstract::Cyclic lipopeptides (CLPs) are produced by many Pseudomonas species and have several biological functions, including a role in surface motility, biofilm formation, virulence, and antimicrobial activity. This study focused on the diversity and role of LuxR-type transcriptional regulators in CLP biosynthesis in Pseudomo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00575-09
更新日期:2009-07-01 00:00:00
abstract::A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.3.804-808.1996
更新日期:1996-03-01 00:00:00
abstract::Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02547-09
更新日期:2010-04-01 00:00:00
abstract::The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02295-18
更新日期:2019-01-09 00:00:00
abstract::The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.1.246-253.1997
更新日期:1997-01-01 00:00:00
abstract::The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous yeast protease. Thi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00412-10
更新日期:2010-07-01 00:00:00
abstract::Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.2.775-782.2000
更新日期:2000-02-01 00:00:00
abstract::Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.11.4808-4813.1999
更新日期:1999-11-01 00:00:00
abstract::The survival of microorganisms can be predicted through the use of active biological containment systems. We have constructed contained Pseudomonas putida strains that degrade alkylbenzoates. The modified strain carries a fusion of the Plac promoter to the gef gene, which encodes a killing protein. Expression from Pla...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.8.2990-2994.1995
更新日期:1995-08-01 00:00:00
abstract::Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.2.369-371.1980
更新日期:1980-02-01 00:00:00
abstract::Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.8.1996-2002.1988
更新日期:1988-08-01 00:00:00
abstract::Inactivation (loss of culturability) by sunlight of enterococci and fecal coliforms within sewage effluent diluted in seawater was investigated in field experiments. In most experiments, 500-ml flasks of pure silica were used to confine activated sludge effluent diluted to 2% (vol/vol) in seawater. Inactivation of bac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.6.2049-2058.1994
更新日期:1994-06-01 00:00:00
abstract::Production of unconventional oil and gas continues to rise, but the effects of high-density hydraulic fracturing (HF) activity near aquatic ecosystems are not fully understood. A commonly used biocide in HF, 2,2-dibromo-3-nitrilopropionamide (DBNPA), was studied in microcosms of HF-impacted (HF+) versus HF-unimpacted ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01336-19
更新日期:2019-10-16 00:00:00
abstract::The rep-PCR DNA fingerprint technique, which uses repetitive intergenic DNA sequences, was investigated as a way to differentiate between human and animal sources of fecal pollution. BOX and REP primers were used to generate DNA fingerprints from Escherichia coli strains isolated from human and animal sources (geese, ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.6.2572-2577.2000
更新日期:2000-06-01 00:00:00
abstract::Pollutant degradation is present mainly in the surface layer of biofilms, and the surface layer is the most vulnerable to impairment by toxic pollutants. In this work, the effects of nanosized TiO2 (n-TiO2) on the average thicknesses of Bacillus subtilis biofilm and on bacterial attachment on different surfaces were i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00047-18
更新日期:2018-04-16 00:00:00
abstract::A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.3.552-555.1986
更新日期:1986-09-01 00:00:00
abstract::Most species of the diazotrophic Proteobacteria Azoarcus spp. occur in association with grass roots, while A. tolulyticus and A. evansii are soil bacteria not associated with a plant host. To facilitate species identification and strain comparison, we developed a protocol for PCR-generated genomic fingerprints, using ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4331-4339.1997
更新日期:1997-11-01 00:00:00
abstract::A mixed methanogenic culture fed with glucose was perturbed with butyrate and formate to investigate the role of formate in the acetogenesis of butyrate. A free energy analysis suggests that formate rather than H(2) was the interspecies electron carrier for butyrate conversion into acetate for the culture studied. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.2.628-630.1993
更新日期:1993-02-01 00:00:00
abstract::Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.9.2771-2775.1992
更新日期:1992-09-01 00:00:00
abstract::The competitive inhibition of fructokinase by glucose has been proposed as the mechanism by which Zymomonas mobilis preferentially consumes glucose from mixtures of glucose and fructose and accumulates fructose when growing on sucrose. In this study, incorporation of radioactive fructose into biomass was used as a mea...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.9.3519-3525.1997
更新日期:1997-09-01 00:00:00
abstract::Small-subunit (SSU) ribosomal DNA (rDNA) gene clusters are phylogenetically related sets of SSU rRNA genes, commonly encountered in genes amplified from natural populations. Genetic variability in gene clusters could result from artifacts (polymerase error or PCR chimera formation), microevolution (variation among rrn...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.1.63-70.1997
更新日期:1997-01-01 00:00:00
abstract::The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01092-10
更新日期:2010-12-01 00:00:00