High-level production of the industrial product lycopene by the photosynthetic bacterium Rhodospirillum rubrum.

abstract：:The control of promoter activity by oxygen availability appears to be an intriguing system for heterologous protein production. In fact, during cell growth in a bioreactor, an oxygen shortage is easily obtained simply by interrupting the air supply. The purpose of our work was to explore the possible use of hypoxic in...

journal_title：Applied and environmental microbiology

authors： Camattari A,Bianchi MM,Branduardi P,Porro D,Brambilla L

更新日期：2007-02-01 00:00:00

abstract：:Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...

journal_title：Applied and environmental microbiology

authors： Wai SN,Mizunoe Y,Takade A,Kawabata SI,Yoshida SI

更新日期：1998-10-01 00:00:00

abstract：:We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a...

journal_title：Applied and environmental microbiology

authors： Rudolph C,Wanner G,Huber R

更新日期：2001-05-01 00:00:00

abstract：:A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B(12). The compounds were isolated from the cells and identified as methylcobalamin (methyl-B(12)) and adenosylcobalamin (adenosyl-B(12)) by various tests. The variation in the form of B(12) during cultivation was examined by bio...

journal_title：Applied and environmental microbiology

authors： Sato K,Ueda S,Shimizu S

更新日期：1977-03-01 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:The genus Aeromonas includes some species that have now been identified as human pathogens of significant medical importance. We investigated the ability of 13 selected Aeromonas strains belonging to nine species isolated from clinical cases (n = 5), environmental waters (n = 5), and fish (n = 3) to adhere to and tran...

journal_title：Applied and environmental microbiology

authors： Hatje E,Neuman C,Katouli M

更新日期：2014-01-01 00:00:00

abstract：:The present work describes the development of a superior strain of Corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown N-acetyldiaminopentane pathway. This acetylated product variant, recently discovered, is a highly undesired by-prod...

journal_title：Applied and environmental microbiology

authors： Kind S,Jeong WK,Schröder H,Zelder O,Wittmann C

更新日期：2010-08-01 00:00:00

abstract：:Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidi...

journal_title：Applied and environmental microbiology

authors： Norman A,Hestbjerg Hansen L,Sørensen SJ

更新日期：2005-05-01 00:00:00

abstract：:The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h de...

journal_title：Applied and environmental microbiology

authors： Plumridge A,Hesse SJ,Watson AJ,Lowe KC,Stratford M,Archer DB

更新日期：2004-06-01 00:00:00

abstract：:We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

journal_title：Applied and environmental microbiology

authors： Graham ML,Springthorpe VS,Sattar SA

更新日期：1996-11-01 00:00:00

abstract：:A total of 530 strains of Staphylococcus aureus were isolated from the defeathering machinery of a chicken processing plant and from neck skin samples of carcasses at different stages of processing in two visits 4 weeks apart. Eleven different plasmid profiles were detected in the isolates, eight being common to both ...

journal_title：Applied and environmental microbiology

authors： Dodd CE,Chaffey BJ,Waites WM

更新日期：1988-06-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. "Heavy" (13)C-labeled DNA, produced after microbial growth on (13)CH(4), was separated from naturally abundant (12)C-DNA by cesium chloride density gradient centrifugation and used as a template fo...

journal_title：Applied and environmental microbiology

authors： Morris SA,Radajewski S,Willison TW,Murrell JC

更新日期：2002-03-01 00:00:00

abstract：:Although extraradicular biofilm formation is related to refractory periapical periodontitis, the mechanism of extraradicular biofilm development, as well as its effect on periapical lesions, is unknown. Therefore, we aimed to develop an in vivo extraradicular biofilm model in rats and to identify and quantify extrarad...

journal_title：Applied and environmental microbiology

authors： Kuremoto K,Noiri Y,Ishimoto T,Yoneda N,Yamamoto R,Maezono H,Nakano T,Hayashi M,Ebisu S

更新日期：2014-07-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:To achieve functional bioluminescence, the developing light organ of newly hatched juveniles of the Hawaiian squid Euprymna scolopes must become colonized by luminous, symbiosis-competent Vibrio fischeri present in the ambient seawater. This benign infection occurs rapidly in animals placed in seawater from the host's...

journal_title：Applied and environmental microbiology

authors： Lee K,Ruby EG

更新日期：1995-01-01 00:00:00

abstract：:The factor in normal sera primarily responsible for the enhancement of transfection (and transformation) of Staphylococcus aureus was identified as fibronectin. Serum samples which were depleted of fibronectin by affinity chromatography showed a marked decrease in enhancing activity. Fibronectin isolated from sera of ...

journal_title：Applied and environmental microbiology

authors： Thompson NE,Bergdoll MS,Pattee PA

更新日期：1985-11-01 00:00:00

abstract：:Pseudomonas syringae is a common foliar bacterium responsible for many important plant diseases. We studied the population structure and dynamics of the core genome of P. syringae via multilocus sequencing typing (MLST) of 60 strains, representing 21 pathovars and 2 nonpathogens, isolated from a variety of plant hosts...

journal_title：Applied and environmental microbiology

authors： Sarkar SF,Guttman DS

更新日期：2004-04-01 00:00:00

abstract：:Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 x 10(4) and 4.5 ...

journal_title：Applied and environmental microbiology

authors： Lumb R,Stapledon R,Scroop A,Bond P,Cunliffe D,Goodwin A,Doyle R,Bastian I

更新日期：2004-08-01 00:00:00

abstract：:We sequenced the Staphylococcus aureus phospho-beta-galactosidase gene. The protein product of this gene consisted of 470 amino acids, giving a molecular weight of 54,557. This gene appears to be transcribed as the terminal sequence on a polycistronic message. ...

journal_title：Applied and environmental microbiology

authors： Breidt F Jr,Stewart GC

更新日期：1987-05-01 00:00:00

abstract：:The degradation of bisphenol A and nonylphenol involves the unusual rearrangement of stable carbon-carbon bonds. Some nonylphenol isomers and bisphenol A possess a quaternary alpha-carbon atom as a common structural feature. The degradation of nonylphenol in Sphingomonas sp. strain TTNP3 occurs via a type II ipso subs...

journal_title：Applied and environmental microbiology

authors： Kolvenbach B,Schlaich N,Raoui Z,Prell J,Zühlke S,Schäffer A,Guengerich FP,Corvini PF

更新日期：2007-08-01 00:00:00

abstract：:The N-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent I) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. The study included concentration, contact time, pH, temperature, and water quality as controlled variables. The species of bacteria teste...

journal_title：Applied and environmental microbiology

authors： Williams DE,Worley SD,Wheatley WB,Swango LJ

更新日期：1985-03-01 00:00:00

abstract：:The bile salt hydrolase activity in intestinal homogenates reflects composite activities of the gastrointestinal microbial consortia. We have proposed that specific transformations of conjugated bile acids by the intestinal microflora result in the production of metabolites which depress the growth of poultry. The inf...

journal_title：Applied and environmental microbiology

authors： Feighner SD,Dashkevicz MP

更新日期：1988-02-01 00:00:00

abstract：:Concentrations of airborne coliform bacteria as high as 1.5 X 10(4) m-3 were observed 8 cm above anaerobically digested sewage sludge applied to a forest clearcut. Dry conditions and high wind speeds tended to favor aerosol generation. ...

journal_title：Applied and environmental microbiology

authors： Edmonds RL,Littke W

更新日期：1978-12-01 00:00:00

abstract：:Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...

journal_title：Applied and environmental microbiology

authors： Napolitano JJ

更新日期：1982-07-01 00:00:00

abstract：:A collection of 13 field isolates of Rhizobium leguminosarum bv. viciae that have the ability to nodulate the roots of current North American cultivars of peas as well as a "primitive" cultivar, Afghanistan, was examined. These isolates originated in diverse geographical regions of the world, which indicates that this...

journal_title：Applied and environmental microbiology

authors： Ma SW,Iyer VN

更新日期：1990-07-01 00:00:00

abstract：:Members of candidate division OP11 are widely distributed in terrestrial and marine ecosystems, yet little information regarding their metabolic capabilities and ecological role within such habitats is currently available. Here, we report on the microfluidic isolation, multiple-displacement-amplification, pyrosequenci...

journal_title：Applied and environmental microbiology

authors： Youssef NH,Blainey PC,Quake SR,Elshahed MS

更新日期：2011-11-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00