Fibronectin enhances transfection of Staphylococcus aureus.

abstract：:Expression of the Saccharomyces cerevisiae DPM1 gene (coding for dolichylphosphate mannose synthase) in Trichoderma reesei (Hypocrea jecorina) increases the intensity of protein glycosylation and secretion and causes ultrastructural changes in the fungal cell wall. In the present work, we undertook further biochemical...

journal_title：Applied and environmental microbiology

authors： Perlińska-Lenart U,Orlowski J,Laudy AE,Zdebska E,Palamarczyk G,Kruszewska JS

更新日期：2006-12-01 00:00:00

abstract：:The importance of hydrogenase activity to corrosion of steel was assessed by using mixed populations of sulfate-reducing bacteria isolated from corroded and noncorroded oil pipelines. Biofilms which developed on the steel studs contained detectable numbers of sulfate-reducing bacteria (10 increasing to 10/0.5 cm). How...

journal_title：Applied and environmental microbiology

authors： Bryant RD,Jansen W,Boivin J,Laishley EJ,Costerton JW

更新日期：1991-10-01 00:00:00

abstract：:RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, a...

journal_title：Applied and environmental microbiology

authors： Kamekura M,Hamakawa T,Onishi H

更新日期：1982-10-01 00:00:00

abstract：:Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including "Candidatus Methylomirabilis oxyfera," and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bact...

journal_title：Applied and environmental microbiology

authors： Luesken FA,Sánchez J,van Alen TA,Sanabria J,Op den Camp HJ,Jetten MS,Kartal B

更新日期：2011-10-01 00:00:00

abstract：:Glycogen branching enzymes (GBE) or 1,4-alpha-glucan branching enzymes (EC 2.4.1.18) introduce alpha-1,6 branching points in alpha-glucans, e.g., glycogen. To identify structural features in GBEs that determine their branching pattern specificity, the Deinococcus geothermalis and Deinococcus radiodurans GBE (GBE(Dg) a...

journal_title：Applied and environmental microbiology

authors： Palomo M,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2009-03-01 00:00:00

abstract：:Eighteen of 1,200 colonies of Escherichia coli isolated from "keebe," hamburger, or sausage produced heat-labile enterotoxin. None of them produced heat-stable enterotoxin. The characteristics of 9 of the 18 strains are presented. ...

journal_title：Applied and environmental microbiology

authors： Reis MH,Vasconcelos JC,Trabulsi LR

更新日期：1980-01-01 00:00:00

abstract：:The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...

journal_title：Applied and environmental microbiology

authors： Shu C,Liu D,Zhou Z,Cai J,Peng Q,Gao J,Song F,Zhang J

更新日期：2013-11-01 00:00:00

abstract：:Reductive and oxidative pathways of the sulfur cycle were studied in a marine sediment by parallel radiotracer experiments with SO(4), H(2)S, and S(2)O(3) injected into undisturbed sediment cores. The distributions of viable populations of sulfate- and thiosulfate-reducing bacteria and of thiosulfate-disproportionatin...

journal_title：Applied and environmental microbiology

authors： Jørgensen BB,Bak F

更新日期：1991-03-01 00:00:00

abstract：:Acid pretreatment is commonly used to release pentoses from the hemicellulose fraction of cellulosic biomass for bioconversion. The predominant pentose in the hemicellulose fraction of hardwoods and crop residues is xylose in the polysaccharide methylglucuronoxylan, in which as many as one in six of the beta-1,4-linke...

journal_title：Applied and environmental microbiology

authors： Bi C,Rice JD,Preston JF

更新日期：2009-01-01 00:00:00

abstract：:The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

journal_title：Applied and environmental microbiology

authors： Enwall K,Philippot L,Hallin S

更新日期：2005-12-01 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Fenton reactions are believed to play important roles in wood degradation by brown rot fungi. In this context, the effect of tropolone (2-hydroxycyclohepta-2,4,6-trienone), a metal chelator, on wood degradation by Poria placenta was investigated. Tropolone (50 micro M) strongly inhibits fungal growth on malt agar, but...

journal_title：Applied and environmental microbiology

authors： Diouf PN,Delbarre N,Perrin D,Gérardin P,Rapin C,Jacquot JP,Gelhaye E

更新日期：2002-09-01 00:00:00

abstract：:A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...

journal_title：Applied and environmental microbiology

authors： Reynolds D,Tranter HS,Sage R,Hambleton P

更新日期：1988-07-01 00:00:00

abstract：:Fecal Candida pintolopesii population levels were found to be significantly affected by laparotomy-inoculation-Bollman apparatus restraint, laparotomy-inoculation, and a milk diet. Gastrectomized rats could not support yeast populations; in intact animals, yeast cells failed to colonize the gastrointestinal tract dist...

journal_title：Applied and environmental microbiology

authors： Artwohl J,McClain A,Cera L

更新日期：1988-10-01 00:00:00

abstract：:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

journal_title：Applied and environmental microbiology

authors： Charles TC,Doty SL,Nester EW

更新日期：1994-11-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00

abstract：:Anaerobic activation of benzene is expected to represent a novel biochemistry of environmental significance. Therefore, benzene metabolism was investigated in Geobacter metallireducens, the only genetically tractable organism known to anaerobically degrade benzene. Trace amounts (<0.5 μM) of phenol accumulated in cult...

journal_title：Applied and environmental microbiology

authors： Zhang T,Tremblay PL,Chaurasia AK,Smith JA,Bain TS,Lovley DR

更新日期：2013-12-01 00:00:00

abstract：:The phylogenetic diversity of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, E.C. 4.1.1.39) large-subunit genes of deep-sea microorganisms was analyzed. Bulk genomic DNA was isolated from seven samples, including samples from the Mid-Atlantic Ridge and various deep-sea habitats around Japan. The kinds o...

journal_title：Applied and environmental microbiology

authors： Elsaied H,Naganuma T

更新日期：2001-04-01 00:00:00

abstract：:The amino acid profiles, modified essential amino acid (MEAA) indexes, and in vitro pepsin digestibilities were determined for single-cell protein (SCP) from certain industrially important lactobacilli. For the three parameters examined, substantial differences were seen between different Lactobacillus species and bet...

journal_title：Applied and environmental microbiology

authors： Erdman MD,Bergen WG,Reddy CA

更新日期：1977-04-01 00:00:00

abstract：:The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...

journal_title：Applied and environmental microbiology

authors： Denef VJ,Mueller RS,Chiang E,Liebig JR,Vanderploeg HA

更新日期：2015-12-18 00:00:00

abstract：:Campylobacter infections are the most common cause of bacterial enteritis in humans, and nearly 8% of such infections are caused by Campylobacter coli. Most studies have concentrated on Campylobacter jejuni, frequently isolated from intensively farmed poultry and livestock production units, and few studies have examin...

journal_title：Applied and environmental microbiology

authors： Leatherbarrow AJ,Hart CA,Kemp R,Williams NJ,Ridley A,Sharma M,Diggle PJ,Wright EJ,Sutherst J,French NP

更新日期：2004-02-01 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:Miniaturized growth systems for heterogeneous culture collections are not only attractive in reducing demands for incubation space and medium but also in making the parallel handling of large numbers of strains more practicable. We report here on the optimization of oxygen transfer rates in deep-well microtiter plates...

journal_title：Applied and environmental microbiology

authors： Duetz WA,Rüedi L,Hermann R,O'Connor K,Büchs J,Witholt B

更新日期：2000-06-01 00:00:00

abstract：:Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

journal_title：Applied and environmental microbiology

authors： Rhodes AN,Fulthorpe RR,Tiedje JM

更新日期：2013-11-01 00:00:00

abstract：:Chemolithotrophic ammonium-oxidizing and nitrite-oxidizing bacteria including Nitrosomonas europaea, Nitrosococcus oceanus, Nitrobacter sp., Nitiospina gracilis, and Nitrococcus mobilis were examined as to their ability to oxidize methane in the absence of ammonium or nitrite. All ammonium oxidizers tested had the abi...

journal_title：Applied and environmental microbiology

authors： Jones RD,Morita RY

更新日期：1983-02-01 00:00:00

abstract：:The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

journal_title：Applied and environmental microbiology

authors： Garcia-Pichel F,Wingard CE,Castenholz RW

更新日期：1993-01-01 00:00:00

abstract：:The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industr...

journal_title：Applied and environmental microbiology

authors： Ginolhac A,Jarrin C,Gillet B,Robe P,Pujic P,Tuphile K,Bertrand H,Vogel TM,Perrière G,Simonet P,Nalin R

更新日期：2004-09-01 00:00:00

abstract：:To enhance food safety and stability, the food industry tends to use natural antimicrobials such as plant-derived compounds as an attractive alternative to chemical preservatives. Nonetheless, caution must be exercised in light of the potential for bacterial adaptation to these molecules, a phenomenon previously obser...

journal_title：Applied and environmental microbiology

authors： Dubois-Brissonnet F,Naïtali M,Mafu AA,Briandet R

更新日期：2011-02-01 00:00:00

abstract：:Hepatitis B virus (HBV) is a deadly pathogen that has killed countless people worldwide. Saccharomyces cerevisiae-derived HBV vaccines based upon hepatitis B surface antigen (HBsAg) is highly effective. However, the emergence of vaccine escape mutants due to mutations on the HBsAg and polymerase genes has produced a c...

journal_title：Applied and environmental microbiology

authors： Yong CY,Yeap SK,Goh ZH,Ho KL,Omar AR,Tan WS

更新日期：2015-02-01 00:00:00