Abstract:
:Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including "Candidatus Methylomirabilis oxyfera," and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescence in situ hybridization and 16S rRNA and pmoA gene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m(3)/day (17.2 mmol day(-1)). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Luesken FA,Sánchez J,van Alen TA,Sanabria J,Op den Camp HJ,Jetten MS,Kartal Bdoi
10.1128/AEM.05539-11subject
Has Abstractpub_date
2011-10-01 00:00:00pages
6802-7issue
19eissn
0099-2240issn
1098-5336pii
AEM.05539-11journal_volume
77pub_type
杂志文章abstract::Two subpopulations of Bacillus megaterium spores (1.360 and 1.355 g/ml) were obtained by density gradient centrifugation. The heavier spores had a higher thermoresistance (e.g., D(80) = 186 versus 81 min) and a higher DNA content (1.25 x 10 versus 0.65 x 10 g per spore, apparently corresponding to digenomic versus mon...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2919-2921.1990
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.1.310-312.1983
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