Simultaneous nitrite-dependent anaerobic methane and ammonium oxidation processes.

Abstract:

:Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including "Candidatus Methylomirabilis oxyfera," and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescence in situ hybridization and 16S rRNA and pmoA gene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m(3)/day (17.2 mmol day(-1)). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.

journal_name

Appl Environ Microbiol

authors

Luesken FA,Sánchez J,van Alen TA,Sanabria J,Op den Camp HJ,Jetten MS,Kartal B

doi

10.1128/AEM.05539-11

subject

Has Abstract

pub_date

2011-10-01 00:00:00

pages

6802-7

issue

19

eissn

0099-2240

issn

1098-5336

pii

AEM.05539-11

journal_volume

77

pub_type

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