A Haloalkane Dehalogenase from a Marine Microbial Consortium Possessing Exceptionally Broad Substrate Specificity.

abstract：:Chemolithotrophic ammonium-oxidizing and nitrite-oxidizing bacteria including Nitrosomonas europaea, Nitrosococcus oceanus, Nitrobacter sp., Nitiospina gracilis, and Nitrococcus mobilis were examined as to their ability to oxidize methane in the absence of ammonium or nitrite. All ammonium oxidizers tested had the abi...

journal_title：Applied and environmental microbiology

authors： Jones RD,Morita RY

更新日期：1983-02-01 00:00:00

abstract：:Specific oxygen uptake rates [Q(02)] were highest when Candida 107 and Rhodotorula gracilis grew at their maximum dilution rates. In nitrogen-limited medium, which encourages lipid accumulation, Q(02) values of both yeasts decreased as the dilution rate was lowered. ...

journal_title：Applied and environmental microbiology

authors： Ratledge C,Hall MJ

更新日期：1977-08-01 00:00:00

abstract：UNLABELLED:Raw sausages are perishable foodstuffs; reducing their salt content raises questions about a possible increased spoilage of these products. In this study, we evaluated the influence of salt reduction (from 2.0% to 1.5% [wt/wt]), in combination with two types of packaging (modified atmosphere [50% mix of CO2-...

journal_title：Applied and environmental microbiology

authors： Fougy L,Desmonts MH,Coeuret G,Fassel C,Hamon E,Hézard B,Champomier-Vergès MC,Chaillou S

更新日期：2016-06-13 00:00:00

abstract：:Thermophilic sulfate-reducing bacteria were enriched from samples obtained from a geothermal underground mine in Japan. The enrichment cultures contained bacteria affiliated with the genera Desulfotomaculum, Thermanaeromonas, Thermincola, Thermovenabulum, Moorella, "Natronoanaerobium," and Clostridium. Two novel therm...

journal_title：Applied and environmental microbiology

authors： Kaksonen AH,Plumb JJ,Robertson WJ,Spring S,Schumann P,Franzmann PD,Puhakka JA

更新日期：2006-05-01 00:00:00

abstract：:Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

journal_title：Applied and environmental microbiology

authors： Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

更新日期：2004-01-01 00:00:00

abstract：:In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly c...

journal_title：Applied and environmental microbiology

authors： Picard C,Ponsonnet C,Paget E,Nesme X,Simonet P

更新日期：1992-09-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance ...

journal_title：Applied and environmental microbiology

authors： Robey M,Benito A,Hutson RH,Pascual C,Park SF,Mackey BM

更新日期：2001-10-01 00:00:00

abstract：:The Amazon rainforest, the largest equatorial forest in the world, is being cleared for pasture and agricultural use at alarming rates. Tropical deforestation is known to cause alterations in microbial communities at taxonomic and phylogenetic levels, but it is unclear whether microbial functional groups are altered. ...

journal_title：Applied and environmental microbiology

authors： Mirza BS,Potisap C,Nüsslein K,Bohannan BJ,Rodrigues JL

更新日期：2014-01-01 00:00:00

abstract：:The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

journal_title：Applied and environmental microbiology

authors： Tsuchido T,Ahn YH,Takano M

更新日期：1987-03-01 00:00:00

abstract：:A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR prime...

journal_title：Applied and environmental microbiology

authors： Lin H,Civerolo EL,Hu R,Barros S,Francis M,Walker MA

更新日期：2005-08-01 00:00:00

abstract：:The chlorine resistance of cysts of the flagellate protozoan Giardia muris was examined. This organism, which is pathogenic to mice, is being considered as a model for the inactivation of the human pathogen Giardia lamblia. Excystation was used as the criterion for cyst viability. Experiments were performed at pH 5, 7...

journal_title：Applied and environmental microbiology

authors： Leahy JG,Rubin AJ,Sproul OJ

更新日期：1987-07-01 00:00:00

abstract：:In in vitro estuarine water and sediment chambers, the survival of Vibrio cholerae and Escherichia coli was determined by plate counting and direct counting techniques. V. cholerae strains included environmental, clinical, and serotype O1 and non-O1 isolates, whereas E. coli strains included ATCC 25922 and a freshly c...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE

更新日期：1982-03-01 00:00:00

abstract：:With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...

journal_title：Applied and environmental microbiology

authors： Restaino L,Myron JJ,Lenovich LM,Bills S,Tscherneff K

更新日期：1984-04-01 00:00:00

abstract：:With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage phiFSW-TI in Escherichia coli. The phiFSW-TI promoter fragment was abutted to the plasmid-borne promoterless bet...

journal_title：Applied and environmental microbiology

authors： Binishofer B,Moll I,Henrich B,Bläsi U

更新日期：2002-08-01 00:00:00

abstract：:Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

journal_title：Applied and environmental microbiology

authors： Wallis PM,Wallis HM

更新日期：1986-03-01 00:00:00

abstract：:The kinetics of concentration-dependent toluene metabolism were examined by evaluating each term in the second-order rate equation. Marine and freshwater pseudomonads were used. Uptake for Pseudomonas sp. strain T2 was characterized by a completely saturatable system with small transport constant (K(t) = 44 mug/liter)...

journal_title：Applied and environmental microbiology

authors： Robertson BR,Button DK

更新日期：1987-09-01 00:00:00

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Agrochemicals, fecal indicator bacteria (FIB), and pathogens frequently contaminate water simultaneously. No significant direct effects of fertilizer, atrazine, malathion, and chlorothalonil on the survival of Escherichia coli, Enterococcus faecalis, Salmonella enterica, human polyomaviruses, and adenovirus were detec...

journal_title：Applied and environmental microbiology

authors： Staley ZR,Senkbeil JK,Rohr JR,Harwood VJ

更新日期：2012-11-01 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the ...

journal_title：Applied and environmental microbiology

authors： Viña-Gonzalez J,Gonzalez-Perez D,Ferreira P,Martinez AT,Alcalde M

更新日期：2015-09-01 00:00:00

abstract：:Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the prese...

journal_title：Applied and environmental microbiology

authors： Brinig MM,Lepp PW,Ouverney CC,Armitage GC,Relman DA

更新日期：2003-03-01 00:00:00

abstract：:Intracellular pH (pH(in)) is a tightly regulated physiological parameter, which controls cell performance in all living systems. The purpose of this work was to evaluate if and how H(+) homeostasis is accomplished by an industrial wine strain of Saccharomyces cerevisiae while fermenting real must under the harsh winer...

journal_title：Applied and environmental microbiology

authors： Viana T,Loureiro-Dias MC,Loureiro V,Prista C

更新日期：2012-09-01 00:00:00

abstract：:The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

journal_title：Applied and environmental microbiology

authors： Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

更新日期：2018-08-31 00:00:00

abstract：:Dehalobacter and "Dehalococcoides" spp. were previously shown to be involved in the biotransformation of 1,1,2-trichloroethane (1,1,2-TCA) and 1,2-dichloroethane (1,2-DCA) to ethene in a mixed anaerobic enrichment culture. Here we report the further enrichment and characterization of a Dehalobacter sp. from this mixed...

journal_title：Applied and environmental microbiology

authors： Grostern A,Edwards EA

更新日期：2009-05-01 00:00:00

abstract：:The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

journal_title：Applied and environmental microbiology

authors： Marquis RE,Bender GR,Murray DR,Wong A

更新日期：1987-01-01 00:00:00

abstract：:During the course of immunologic studies involving the gastrointestinal colonization of mice with Candida albicans, it became apparent that the animals were being exposed to large numbers of Aspergillus fumigatus spores which interfered with the C. albicans colonization. To determine the source of the A. fumigatus exp...

journal_title：Applied and environmental microbiology

authors： Mayeux P,Dupepe L,Dunn K,Balsamo J,Domer J

更新日期：1995-06-01 00:00:00

abstract：:Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

journal_title：Applied and environmental microbiology

authors： Katsuwon J,Anderson AJ

更新日期：1990-11-01 00:00:00

abstract：:Quorum sensing (QS) regulates Phaeobacter gallaeciensis antagonism in broth systems; however, we demonstrate here that QS is not important for antagonism in algal cultures. QS mutants reduced Vibrio anguillarum to the same extent as the wild type. Consequently, a combination of probiotic Phaeobacter and QS inhibitors ...

journal_title：Applied and environmental microbiology

authors： Prol García MJ,D'Alvise PW,Gram L

更新日期：2013-09-01 00:00:00

abstract：:The biological succession that occurs during the first year of life in the gut of infants in Western countries is broadly predictable in terms of the increasing complexity of the composition of microbiotas. Less information is available about microbiotas in Asian countries, where environmental, nutritional, and cultur...

journal_title：Applied and environmental microbiology

authors： Lawley B,Otal A,Moloney-Geany K,Diana A,Houghton L,Heath AM,Taylor RW,Tannock GW

更新日期：2019-09-17 00:00:00