Lysis of Bacillus subtilis Cells by Glycerol and Sucrose Esters of Fatty Acids.

abstract：:Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

journal_title：Applied and environmental microbiology

authors： Brown JC,Huang G,Haley-Zitlin V,Jiang X

更新日期：2009-02-01 00:00:00

abstract：:Pseudomonas aeruginosa exhibits flagellum-mediated swimming in liquid and swarming on hydrated surfaces under diverse nutrient conditions. Prior studies have implicated a phosphodiesterase, DipA, in regulating these flagellum-mediated motilities, but collectively, the necessity for DipA was unclear. In this study, we ...

journal_title：Applied and environmental microbiology

authors： Mattingly AE,Kamatkar NG,Morales-Soto N,Borlee BR,Shrout JD

更新日期：2018-03-19 00:00:00

abstract：:Approximately 70% of the cells in a suspension of the amylolytic bacterium Lactobacillus amylovorus bind to cornstarch granules within 30 min at 25 degrees C. More than 60% of the bound bacteria were removed by formaldehyde (2%) or glycine (1 M) at pH 2.0. More than 90% of the bound bacteria were removed by MgCl(2) (2...

journal_title：Applied and environmental microbiology

authors： Imam SH,Harry-O'kuru RE

更新日期：1991-04-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:The spore-forming bacterium Paenibacillus larvae causes a severe and highly infective bee disease, American foulbrood (AFB). Despite the large economic losses induced by AFB, the virulence factors produced by P. larvae are as yet unknown. To identify such virulence factors, we experimentally infected young, susceptibl...

journal_title：Applied and environmental microbiology

authors： Schild HA,Fuchs SW,Bode HB,Grünewald B

更新日期：2014-04-01 00:00:00

abstract：:Previously, members of our group reported the isolation and characterization of mutacin II from Streptococcus mutans T8 and the genetic analyses of the mutacin II biosynthesis genes (J. Novak, P. W. Caufield, and E. J. Miller, J. Bacteriol. 176:4316-4320, 1994; F. Qi, P. Chen, and P. W. Caufield, Appl. Environ. Microb...

journal_title：Applied and environmental microbiology

authors： Qi F,Chen P,Caufield PW

更新日期：1999-09-01 00:00:00

abstract：:Inhibition of Aspergillus flavus growth from spores was used as a simple bioassay for toxicity of Al and Mn mobilized by simulated acid precipitation. Al was identified as being toxic in soil leachates resulting from acid inputs of pH less than 2.7. Inhibition by Mn was not detectable. The addition of fluoride signifi...

journal_title：Applied and environmental microbiology

authors： Firestone MK,Killham K,McColl JG

更新日期：1983-09-01 00:00:00

abstract：:The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 ...

journal_title：Applied and environmental microbiology

authors： Hong Y,Xu M,Guo J,Xu Z,Chen X,Sun G

更新日期：2007-01-01 00:00:00

abstract：:A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and...

journal_title：Applied and environmental microbiology

authors： Aurell H,Catala P,Farge P,Wallet F,Le Brun M,Helbig JH,Jarraud S,Lebaron P

更新日期：2004-03-01 00:00:00

abstract：:The increased demand for sterile products has created the need for rapid technologies capable of validating the hygiene of industrial production processes. Bacillus endospores are in standard use as biological indicators for evaluating the effectiveness of sterilization processes. Currently, culture-based methods, req...

journal_title：Applied and environmental microbiology

authors： Yung PT,Ponce A

更新日期：2008-12-01 00:00:00

abstract：:The fermentative metabolism of glucose was redirected to succinate as the primary product without mutating any genes encoding the native mixed-acid fermentation pathway or redox reactions. Two changes in peripheral pathways were together found to increase succinate yield fivefold: (i) increased expression of phosphoen...

journal_title：Applied and environmental microbiology

authors： Zhang X,Jantama K,Shanmugam KT,Ingram LO

更新日期：2009-12-01 00:00:00

abstract：:In this paper, the ability of a commercial starter culture to perform a sausage fermentation is evaluated. Molecular analysis revealed the presence of several strains of the same species contained in the starter culture with different behavior during the fermentation, and the contribution of Lactobacillus curvatus, wh...

journal_title：Applied and environmental microbiology

authors： Cocolin L,Urso R,Rantsiou K,Cantoni C,Comi G

更新日期：2006-01-01 00:00:00

abstract：:Recently, the lignin-degrading basidiomycete Pleurotus ostreatus has become a widely used model organism for fungal genomic and transcriptomic analyses. The increasing interest in this species has led to an increasing number of studies analyzing the transcriptional regulation of multigene families that encode extracel...

journal_title：Applied and environmental microbiology

authors： Castanera R,López-Varas L,Pisabarro AG,Ramírez L

更新日期：2015-06-15 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

journal_title：Applied and environmental microbiology

authors： Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

更新日期：2000-09-01 00:00:00

abstract：:Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...

journal_title：Applied and environmental microbiology

authors： Alvarez B,Secades P,McBride MJ,Guijarro JA

更新日期：2004-01-01 00:00:00

abstract：:We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for...

journal_title：Applied and environmental microbiology

authors： Yaradou DF,Hallier-Soulier S,Moreau S,Poty F,Hillion Y,Reyrolle M,André J,Festoc G,Delabre K,Vandenesch F,Etienne J,Jarraud S

更新日期：2007-03-01 00:00:00

abstract：:In contrast to extensive studies of phosphorus, widely considered the main nutrient limiting phytoplankton biomass in freshwater ecosystems, there have been few studies on the role of nitrogen in controlling phytoplankton populations. This situation may be due partly to the complexity in estimating its utilization and...

journal_title：Applied and environmental microbiology

authors： Gillor O,Harush A,Hadas O,Post AF,Belkin S

更新日期：2003-03-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:Comparative genomic hybridizations have been used to examine genetic relationships among bacteria. The microarrays used in these experiments may have open reading frames from one or more reference strains (whole-genome microarrays), or they may be composed of random DNA fragments from a large number of strains (mixed-...

journal_title：Applied and environmental microbiology

authors： Wan Y,Broschat SL,Call DR

更新日期：2007-03-01 00:00:00

abstract：:The photosynthetic bacterium Rhodobacter sphaeroides is capable of producing H2 via nitrogenase when grown photoheterotrophically in the absence of N2. By using 14C-labeled malate, it was found that greater than 95% of this substrate was catabolized completely to CO2 during H2 production. About 60% of this catabolism ...

journal_title：Applied and environmental microbiology

authors： Macler BA,Bassham JA

更新日期：1988-11-01 00:00:00

abstract：:Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

journal_title：Applied and environmental microbiology

authors： Chu FS,Huang X,Wei RD,Carmichael WW

更新日期：1989-08-01 00:00:00

abstract：:A high-throughput method which is applicable for rapid screening, identification, and delineation of isolates of Vibrio cholerae, sensitive to genome variation, and capable of providing phylogenetic inferences enhances environmental monitoring of this bacterium. We have developed and optimized a method for genomic fin...

journal_title：Applied and environmental microbiology

authors： Chokesajjawatee N,Zo YG,Colwell RR

更新日期：2008-09-01 00:00:00

abstract：:We previously showed a pivotal role of the polysaccharide (PS) moiety in the cell wall of the Lactobacillus casei strain Shirota (YIT 9029) as a possible immune modulator (E. Yasuda M. Serata, and T. Sako, Appl. Environ. Microbiol. 74:4746-4755, 2008). To distinguish PS structures on the bacterial cell surface of indi...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Tateno H,Hirabayashi J,Iino T,Sako T

更新日期：2011-07-01 00:00:00

abstract：:Growth and succinate versus lactate production from glucose by Anaerobiospirillum succiniciproducens was regulated by the level of available carbon dioxide and culture pH. At pH 7.2, the generation time was almost doubled and extensive amounts of lactate were formed in comparison with growth at pH 6.2. The succinate y...

journal_title：Applied and environmental microbiology

authors： Samuelov NS,Lamed R,Lowe S,Zeikus JG

更新日期：1991-10-01 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:A seasonal study of the distribution of lysogenic bacteria in Tampa Bay, Florida, was conducted over a 13-month period. Biweekly water samples were collected and either were left unaltered or had the viral population reduced by filtration (pore size, 0.2 micro m) and resuspension in filtered (pore size, 0.2 micro m) w...

journal_title：Applied and environmental microbiology

authors： Williamson SJ,Houchin LA,McDaniel L,Paul JH

更新日期：2002-09-01 00:00:00

abstract：:Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and electrophoresed in a...

journal_title：Applied and environmental microbiology

authors： Mahony DE,Clark GA,Stringer MF,MacDonald MC,Duchesne DR,Mader JA

更新日期：1986-03-01 00:00:00

abstract：:The prophage-encoded Shiga toxin is a major virulence factor in Stx-producing Escherichia coli (STEC). Toxin production and phage production are linked and occur after induction of the RecA-dependent SOS response. However, food-related stress and Stx-prophage induction have not been studied at the single-cell level. T...

journal_title：Applied and environmental microbiology

authors： Fang Y,Mercer RG,McMullen LM,Gänzle MG

更新日期：2017-09-15 00:00:00

abstract：:Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region aff...

journal_title：Applied and environmental microbiology

authors： Yang H,Shi P,Liu Y,Xia W,Wang X,Cao H,Ma R,Luo H,Bai Y,Yao B

更新日期：2017-03-02 00:00:00