Abstract:
:Approximately 70% of the cells in a suspension of the amylolytic bacterium Lactobacillus amylovorus bind to cornstarch granules within 30 min at 25 degrees C. More than 60% of the bound bacteria were removed by formaldehyde (2%) or glycine (1 M) at pH 2.0. More than 90% of the bound bacteria were removed by MgCl(2) (2 M; pH 7.0). Binding of L. amylovorus to cornstarch was inhibited in heat-killed cells and in cells that had been pretreated with glutaraldehyde, formaldehyde, sodium azide, trypsin, or 1% soluble potato starch. Bacterial binding to cornstarch appeared to correlate with both the concentration of cornstarch in the suspension and the amylose content in the granules. The ability of L. amylovorus to adhere to cornstarch granules was reduced for granules that had been extracted with HCl-ethanol, HCl-methanol, HCl-propanol, or HCl-butanol. Chemical derivatization of cornstarch resulted in a wide variety of adhesion responses by these bacteria. For example, 2-O-butyl starch (degree of substitution, 0.09) enhanced adhesion, whereas two palmitate starches (degree of substitution, 0.48 and 0.09) exhibited reduced adhesion activities. 2-O-(2-hydroxybutyl) starch and starch-poly(ethylene-co-acrylic acid) ester showed adhesion activities similar to those of the nonderivatized starch controls.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Imam SH,Harry-O'kuru REdoi
10.1128/AEM.57.4.1128-1133.1991keywords:
subject
Has Abstractpub_date
1991-04-01 00:00:00pages
1128-33issue
4eissn
0099-2240issn
1098-5336journal_volume
57pub_type
杂志文章abstract::Biosensor technology has a great potential to meet the need for sensitive and nearly real-time microbial detection from foods. An antibody-based fiber-optic biosensor to detect low levels of Listeria monocytogenes cells following an enrichment step was developed. The principle of the sensor is a sandwich immunoassay w...
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doi:10.1128/AEM.44.2.499-501.1982
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.46.4.826-831.1983
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.55.9.2308-2314.1989
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doi:10.1128/AEM.50.4.958-964.1985
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doi:10.1128/AEM.55.11.2877-2881.1989
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.55.2.520-522.1989
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.6.1746-1752.1991
更新日期:1991-06-01 00:00:00
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